Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.)

Mendoza De Gyves, E., Sparks, CarolineORCID logo, Sayanova, Olga, Lazzeri, P. A., Napier, JohnathanORCID logo and Jones, Huw (2004) Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.). Plant Biotechnology Journal, 2 (4). pp. 351-357. 10.1111/j.1467-7652.2004.00079.x
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A robust Agrobacterium-mediated transformation procedure was developed for Rigel, a commercial cultivar of evening primrose, and used to deliver a cDNA encoding a Delta(6)-desaturase from borage under the control of a cauliflower mosaic virus (CaMV) 35S promoter. Analysis of the transformed plants demonstrated an altered profile of polyunsaturated fatty acids, with an increase in gamma-linolenic acid and octadecatetraenoic acid in leaf tissues when compared with control lines.
Item Type Article
Open Access Not Open Access
Additional information Rothamsted Res, CPI Div, Harpenden AL5 2JQ, Herts, England
Keywords Biotechnology & Applied Microbiology, Plant Sciences
Project 503, 501, Project: 4546, Development and application of cereal transformation technologies
Date Deposited 05 Dec 2025 09:34
Last Modified 21 Jan 2026 17:18

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