Data analysis for 16S microbial profiling from different benchtop sequencing platforms
Pylro, V. S., Roesch, L. F. W., Morais, D. K., Clark, Ian
, Hirsch, Penny
and Totola, M. R.
(2014)
Data analysis for 16S microbial profiling from different benchtop sequencing platforms.
Journal of Microbiological Methods, 107 (Decemb).
pp. 30-37.
10.1016/j.mimet.2014.08.018
Progress in microbial ecology is confounded by problems when evaluating results from different sequencing methodologies. Contrary to existing expectations, here we demonstrate that the same biological conclusion is reached using different NGS technologies when stringent sequence quality filtering and accurate clustering algorithms are applied.
| Item Type | Article |
|---|---|
| Open Access | Gold |
| Keywords | Next generation sequencing, Microbial community analysis, Amplicons, Alpha diversity, Beta diversity |
| Project | Delivering Sustainable Systems (SS) [ISPG], Optimisation of nutrients in soil-plant systems: How can we control nitrogen cycling in soil? |
| Date Deposited | 05 Dec 2025 09:50 |
| Last Modified | 19 Dec 2025 14:35 |
ORCID: https://orcid.org/0000-0002-1589-5420
ORCID: https://orcid.org/0000-0002-5909-1934


