Some effects of changing temperature and of virus inhibitors on infection by cucumber mosaic virus

Whereas the spinach strain of cucumber mosaic virus fails to multiply and cause symptoms in tobacco plants kept above 30 degrees C., the yellow strain infects at 36 degrees C. and causes more severe symptoms than at 20 degrees C. Increasing the temperature up to 28 degrees C. increases the initial rate at which the spinach strain multiplies, but the virus later reaches much higher concentrations in leaves at lower temperatures, presumably because it is rapidly inactivated at 28 degrees C. Exposing inoculated plants to 36 degrees C. for 6 hr. decreases the number of infections by the spinach strain when the exposure starts within 6 hr. of inoculation, but not afterwards. Pancreatic ribonuclease inhibits infections by strains of cucumber mosaic virus; inhibition is greatest when the enzyme is present in the inoculum, and when applied to inoculated leaves its effect decreases rapidly with increasing time after inoculation. Infection by and the multiplication of strains of cucumber mosaic virus in tobacco are only slightly affected by thiouracil and greatly by azaguanine, whereas strains of tobacco mosaic virus are inhibited much more by thiouracil than by azaguanine. Like tobacco mosaic virus, cucumber mosaic virus multiplies more when inoculated leaves are floated in nutrient solutions than in water, but unlike tobacco mosaic virus, its multiplication is not inhibited by thiouracil more in nutrient solutions than in water.