Studies on American foul brood of bees II. The germination of the endospores of bacillus larvae in media containing embryonic tissues

Summary The tissues of the developing chicken embryo form a more suitable substrate for the germination of the endospores of Bacillus larvae than any medium so far described. The addition of beef digest broth to a medium of minced chicken embryo hinders rather than promotes the germination of B. larvae spores. Concentrations of reducing sugars as high as 12.5% cause no apparent hinderance in the germination of B. larvae spores in embryo medium.