Genome-Wide In Silico Analysis of Leucine-Rich Repeat R-Genes in Perilla citriodora: Classification and Expression Insights

Background: Resistance (R) genes are crucial for defending Perilla against pathogens like anthracnose, downy mildew, and phytophthora blight. Nucleotide-binding site leucine-rich repeat (NBS-LRR) genes, the largest R-gene family, play a central role in immunity. This study aimed to identify and characterize NBS-LRR genes in P. citriodora ‘Jeju17’. Methods: Previously conducted genome-wide data for ‘Jeju17’ were analyzed in silico to identify NBS-LRR genes. Results: A total of 535 NBS-LRR genes were identified,with clusters on chromosomes 2, 4, and 10. A unique RPW8-type R-gene was located on chromosome 7.

Conclusions: This study provides insights into the NBS-LRR gene family in ‘Je-ju17’, highlighting its role in disease resistance and evolutionary dynamics. By identifying candidate R-genes, this research supports breeding programs to develop disease-resistant cultivars and improves our understanding of plant immunity.