Development of an acute and chronic ecotoxicity assay using lux -marked Rhizobium leguminosarum biovar trifolii
Paton, G. I., Palmer, G., Burton, M., Rattray, E. A. S., McGrath, Steve
, Glover, L. A. and Killham, K.
(1997)
Development of an acute and chronic ecotoxicity assay using lux -marked Rhizobium leguminosarum biovar trifolii.
Letters in Applied Microbiology, 24 (4).
pp. 296-300.
10.1046/j.1472-765X.1997.00071.x
, Glover, L. A. and Killham, K.
(1997)
Development of an acute and chronic ecotoxicity assay using lux -marked Rhizobium leguminosarum biovar trifolii.
Letters in Applied Microbiology, 24 (4).
pp. 296-300.
10.1046/j.1472-765X.1997.00071.x
A soil isolate of Rhizobium leguminosarum bv. trifolii was marked with a lux CDABE gene cassette to enable the expression of bioluminescence. The suitability of the bacterium as a soil pollution biosensor was assessed using acute and chronic assays. Bacterial bioluminescence responded sensitively to the metals studied, The order of sensitivity was found to be Cd > Ni = Zn > Cu for the acute test and Cd > Ni = Zn = Cu for the chronic test. The sensitive response of the biosensor highlighted its potential for use as an indicator of soil pollution.
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