A - Papers appearing in refereed journals
Zhang, H., Gannon, L., Hassall, K. L., Deery, M. J., Gibbs, D. J., Holdsworth, M. J., Van der Hoorn, R. A. L., Lilley, K. S. and Theodoulou, F. L. 2018. N-terminomics reveals control of Arabidopsis seed storage proteins and proteases by the Arg/N-end rule pathway. New Phytologist. 218 (3), pp. 1106-1126. https://doi.org/10.1111/nph.14909
Authors | Zhang, H., Gannon, L., Hassall, K. L., Deery, M. J., Gibbs, D. J., Holdsworth, M. J., Van der Hoorn, R. A. L., Lilley, K. S. and Theodoulou, F. L. |
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Abstract | The N-end rule pathway of targeted protein degradation is an important regulator of diverse processes in plants but detailed knowledge regarding its influence on the proteome is lacking. To investigate the impact of the Arg/N-end rule pathway on the proteome of etiolated seedlings, we used terminal amine isotopic labelling of substrates with tandem mass tags (TMT-TAILS) for relative quantification of N-terminal peptides in prt6, an Arabidopsis thaliana N-end rule mutant lacking the E3 ligase PROTEOLYSIS6 (PRT6). |
Keywords | Arabidopsis thaliana; Cruciferin; N-end rule; N-terminomics; Protease; Quantitative proteomics; TAILS; Tandem mass tag (TMT) |
Year of Publication | 2018 |
Journal | New Phytologist |
Journal citation | 218 (3), pp. 1106-1126 |
Digital Object Identifier (DOI) | https://doi.org/10.1111/nph.14909 |
Open access | Published as ‘gold’ (paid) open access |
Funder | Biotechnology and Biological Sciences Research Council |
Funder project or code | Substrates of the N-end rule of targeted protein degradation |
TPM - Tailoring Plant Metabolism - Work package 1 (WP1) - High value lipids for health and industry | |
Publisher's version | |
Output status | Published |
Publication dates | |
Online | 23 Nov 2017 |
Publication process dates | |
Accepted | 23 Oct 2017 |
Publisher | Wiley |
Wiley-Blackwell | |
Copyright license | CC BY |
ISSN | 0028-646X |
Permalink - https://repository.rothamsted.ac.uk/item/84563/n-terminomics-reveals-control-of-arabidopsis-seed-storage-proteins-and-proteases-by-the-arg-n-end-rule-pathway