Land-use influences phosphatase gene microdiversity in soils

A - Papers appearing in refereed journals

Neal, A. L., Rossmann, M., Brearley, C., Akkari, E., Guyomar, C., Clark, I. M., Allen, E. and Hirsch, P. R. 2017. Land-use influences phosphatase gene microdiversity in soils. Environmental Microbiology. 19 (7), p. 2740–2753.

AuthorsNeal, A. L., Rossmann, M., Brearley, C., Akkari, E., Guyomar, C., Clark, I. M., Allen, E. and Hirsch, P. R.
Abstract

Phosphorus cycling exerts significant influence upon soil fertility and productivity – processes largely controlled by microbial activity. We adopted phenotypic and metagenomic approaches to investigate phosphatase genes within soils. Microbial communities in bare fallowed soil showed a marked capacity to utilise phytate for growth compared with arable or grassland soil communities. Bare fallowed soil contained lowest concentrations of orthophosphate. Analysis of metagenomes indicated phoA, phoD and phoX, and histidine acid and cysteine phytase genes were most abundant in grassland soil which contained the greatest amount of NaOH-EDTA extractable orthophosphate. Beta-propeller phytase genes were most abundant in bare fallowed soil. Phylogenetic analysis of metagenome sequences indicated the phenotypic shift observed in the capacity to mineralise phytate in bare fallow soil was accompanied by an increase in phoD, phoX and beta-propeller phytase genes coding for exoenzymes. However, there was a remarkable degree of genetic similarity across the soils despite the differences in land-use. Predicted extracellular ecotypes were distributed across a greater range of soil structure than predicted intracellular ecotypes, suggesting that microbial communities subject to the dual stresses of low nutrient availability and reduced access to organic material in bare fallowed soils rely upon the action of exoenzymes.

KeywordsPhytase metagenomics; Phosphatase; Soil
Year of Publication2017
JournalEnvironmental Microbiology
Journal citation19 (7), p. 2740–2753
Digital Object Identifier (DOI)doi:10.1111/1462-2920.13778
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeOptimisation of nutrients in soil-plant systems: How can we control nitrogen cycling in soil?
Sustainability
Publisher's version
Output statusPublished
Publication dates
Online30 May 2017
Publication process dates
Accepted18 Apr 2018
PublisherWiley-Blackwell
Wiley
Copyright licenseCC BY
ISSN1462-2912

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