Expression of lima bean terpene synthases in rice enhances recruitment of a beneficial enemy of a major rice pest

A - Papers appearing in refereed journals

Li, F., Li, W., Lin, Y-J., Pickett, J. A., Birkett, M. A., Wu, K., Wang, G. and Zhou, J. 2018. Expression of lima bean terpene synthases in rice enhances recruitment of a beneficial enemy of a major rice pest. Plant, Cell & Environment. 41 (1), pp. 111-120.

AuthorsLi, F., Li, W., Lin, Y-J., Pickett, J. A., Birkett, M. A., Wu, K., Wang, G. and Zhou, J.
Abstract

Volatile terpenoids play a key role in plant defence against herbivory by attracting parasitic wasps. We identified seven terpene synthase genes from lima bean, Phaseolus lunatus L. following treatment with either the elicitor alamethicin or spider mites, Tetranychus cinnabarinus. Four of the genes (Pltps2, Pltps3, Pltps4 and Pltps5) were up-regulated with their derived proteins phylogenetically clustered in the TPS-g subfamily and PlTPS3 positioned at the base of this cluster. Recombinant PlTPS3 was able to convert geranyl diphosphate and farnesyl diphosphate to linalool and (E)-nerolidol, the latter being precursor of the homoterpene (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT). Recombinant PlTPS4 showed a different substrate specificity and produced linalool and (E)-nerolidol, as well as (E,E)-geranyllinalool from geranylgeranyl diphosphate. Transgenic rice expressing Pltps3 emitted significantly more (S)-linalool and DMNT than wild-type plants, whereas transgenic rice expressing Pltps4 produced (S)-linalool, DMNT and (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT). In laboratory bioassays, female Cotesia chilonis, the natural enemy of the striped rice stemborer, Chilo suppressalis, were significantly attracted to the transgenic plants and their volatiles. We further confirmed this with synthetic blends mimicking natural rice volatile composition. Our study demonstrates that the transformation of rice to produce volatile terpenoids has the potential to enhance plant indirect defence through natural enemy recruitment

Keywords(E)-4,8-dimethyl-1,3,7-nonatriene (DMNT; Parasitic wasp; Trangenic rice
Year of Publication2018
JournalPlant, Cell & Environment
Journal citation41 (1), pp. 111-120
Digital Object Identifier (DOI)doi:10.1111/pce.12959
PubMed ID28370092
Open accessPublished as non-open access
Funder project or codeCUKPGP:New pest resistance in rice, by breeding and genetic modification (GM) for constitutive and inducible levels of defence homoterpenes
FunderBiotechnology and Biological Sciences Research Council
Output statusPublished
Publication dates
Online30 Mar 2017
Publication process dates
Accepted24 Mar 2017
Copyright licenseCC BY
PublisherWiley-Blackwell
ISSN0140-7791

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