Sequence diversity and Identification of novel puroindoline and grain softness protein alleles in Elymus, Agropyron and related species

A - Papers appearing in refereed journals

Wilkinson, M. D., King, R. and Grimaldi, R. 2018. Sequence diversity and Identification of novel puroindoline and grain softness protein alleles in Elymus, Agropyron and related species. Diversity. 10 (4), p. 114.

AuthorsWilkinson, M. D., King, R. and Grimaldi, R.
Abstract

The puroindoline proteins, PINA and PINB, which are encoded by the Pina and Pinb genes located at the Ha locus on chromosome 5D of bread wheat, are considered to be the most important determinants of grain hardness, but the recent identification of Pinb-2 genes on group 7 chromosomes has stressed the importance of considering the effects of related genes and proteins. Several species related to wheat (two diploid Agropyron spp., four tetraploid Elymus spp. and five hexaploid Elymus and Agropyron spp.) were therefore analyzed to identify novel variation in Pina, Pinb and Pinb-2 genes which could be exploited for the improvement of cultivated wheat. A novel sequence for the Pina gene was detected in Elymus burchan-buddae, Elymus dahuricus subsp. excelsus and Elymus nutans and novel PINB sequences in Elymus burchan-buddae, Elymus dahuricus subsp. excelsus, and Elymus nutans. A novel PINB-2 variant was also detected in Agropyron repens and Elymus repens. The encoded proteins detected all showed changes in the tryptophan-rich domain as well as changes and/or deletions of basic and hydrophobic residues. In addition, two new AGP sequences were identified in Elymus nutans and Elymus wawawaiensis. The data presented therefore highlights the sequence diversity in this important gene family and the potential to exploit this diversity to modify grain texture and end-use quality in wheat.

KeywordsElymus species; Puroindoline genes; Pinb-2 genes; novel sequence variants
Year of Publication2018
JournalDiversity
Journal citation10 (4), p. 114
Digital Object Identifier (DOI)doi:10.3390/d10040114
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDesigning Future Wheat (DFW) [ISPG]
DFW - Designing Future Wheat - Work package 1 (WP1) - Increased efficiency and sustainability
Publisher's version
Output statusPublished
Publication dates
Online18 Oct 2018
Publication process dates
Accepted11 Oct 2018
PublisherMDPI
Copyright licenseCC BY
ISSN1424-2818

Permalink - https://repository.rothamsted.ac.uk/item/84v07/sequence-diversity-and-identification-of-novel-puroindoline-and-grain-softness-protein-alleles-in-elymus-agropyron-and-related-species

17 total views
16 total downloads
0 views this month
0 downloads this month
Download files as zip