Natural variation in acyl editing is a determinant of seed storage oil composition

A - Papers appearing in refereed journals

Menard, G. N., Bryant, F. M., Kelly, A. A., Craddock, C. P., Lavagi, I., Hassani-Pak, K., Kurup, S. and Eastmond, P. J. 2018. Natural variation in acyl editing is a determinant of seed storage oil composition. Scientific Reports. 8, p. 17346.

AuthorsMenard, G. N., Bryant, F. M., Kelly, A. A., Craddock, C. P., Lavagi, I., Hassani-Pak, K., Kurup, S. and Eastmond, P. J.
Abstract

Seeds exhibit wide variation in the fatty acid composition of their storage oil. However, the genetic basis of this variation is only partially understood. Here we have used a multi-parent advanced generation inter-cross (MAGIC) population to study the genetic control of fatty acid chain length in Arabidopsis thaliana seed oil. We mapped four quantitative trait loci (QTL) for the quantity of the major very long chain fatty acid species 11-eicosenoic acid (20:1), using multiple QTL modelling. Surprisingly, the main-effect QTL does not coincide with FATTY ACID ELONGASE1 and a parallel genome wide association study suggested that LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASE2 (LPCAT2) is a candidate for this QTL. Regression analysis also suggested that LPCAT2 expression and 20:1 content in seeds of the 19 MAGIC founder accessions are related. LPCAT is a key component of the Lands cycle; an acyl-editing pathway that enables acyl-exchange between the acyl-Coenzyme A and phosphatidylcholine precursor pools used for microsomal fatty acid elongation and desaturation, respectively. We Mendelianised the main-effect QTL using biparental chromosome segment substitution lines and carried out complementation tests to show that a single cis-acting polymorphism in the LPCAT2 promoter causes the variation in seed 20:1 content, by altering the LPCAT2 expression level and total LPCAT activity in developing siliques. Our work establishes that oilseed species exhibit natural variation in the enzymic capacity for acyl-editing and this contributes to the genetic control of storage oil composition.

Year of Publication2018
JournalScientific Reports
Journal citation8, p. 17346
Digital Object Identifier (DOI)doi:10.1038/s41598-018-35136-6
PubMed ID30478395
PubMed Central IDPMC6255774
Open accessPublished as ‘gold’ (paid) open access
FunderBBSRC Industrial Strategy Challenge
Biotechnology and Biological Sciences Research Council
Funder project or codeTailoring Plant Metabolism ISP
Understanding the role of lipases in controlling seed storage oil composition
TPM - Tailoring Plant Metabolism - Work package 1 (WP1) - High value lipids for health and industry
Publisher's version
Copyright license
CC BY
Accepted author manuscript
Copyright license
CC BY
Supplemental file
Copyright license
CC BY
Output statusPublished
Publication dates
Online26 Nov 2018
Publication process dates
Accepted26 Oct 2018
PublisherNature Publishing Group
ISSN2045-2322

Permalink - https://repository.rothamsted.ac.uk/item/84v33/natural-variation-in-acyl-editing-is-a-determinant-of-seed-storage-oil-composition

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