Natural variation in acyl editing is a determinant of seed storage oil composition
A - Papers appearing in refereed journals
Menard, G. N., Bryant, F. M., Kelly, A. A., Craddock, C. P., Lavagi, I., Hassani-Pak, K., Kurup, S. and Eastmond, P. J. 2018. Natural variation in acyl editing is a determinant of seed storage oil composition. Scientific Reports. 8, p. 17346. https://doi.org/10.1038/s41598-018-35136-6
| Authors | Menard, G. N., Bryant, F. M., Kelly, A. A., Craddock, C. P., Lavagi, I., Hassani-Pak, K., Kurup, S. and Eastmond, P. J. |
|---|---|
| Abstract | Seeds exhibit wide variation in the fatty acid composition of their storage oil. However, the genetic basis of this variation is only partially understood. Here we have used a multi-parent advanced generation inter-cross (MAGIC) population to study the genetic control of fatty acid chain length in Arabidopsis thaliana seed oil. We mapped four quantitative trait loci (QTL) for the quantity of the major very long chain fatty acid species 11-eicosenoic acid (20:1), using multiple QTL modelling. Surprisingly, the main-effect QTL does not coincide with FATTY ACID ELONGASE1 and a parallel genome wide association study suggested that LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASE2 (LPCAT2) is a candidate for this QTL. Regression analysis also suggested that LPCAT2 expression and 20:1 content in seeds of the 19 MAGIC founder accessions are related. LPCAT is a key component of the Lands cycle; an acyl-editing pathway that enables acyl-exchange between the acyl-Coenzyme A and phosphatidylcholine precursor pools used for microsomal fatty acid elongation and desaturation, respectively. We Mendelianised the main-effect QTL using biparental chromosome segment substitution lines and carried out complementation tests to show that a single cis-acting polymorphism in the LPCAT2 promoter causes the variation in seed 20:1 content, by altering the LPCAT2 expression level and total LPCAT activity in developing siliques. Our work establishes that oilseed species exhibit natural variation in the enzymic capacity for acyl-editing and this contributes to the genetic control of storage oil composition. |
| Year of Publication | 2018 |
| Journal | Scientific Reports |
| Journal citation | 8, p. 17346 |
| Digital Object Identifier (DOI) | https://doi.org/10.1038/s41598-018-35136-6 |
| PubMed ID | 30478395 |
| PubMed Central ID | PMC6255774 |
| Open access | Published as ‘gold’ (paid) open access |
| Funder | BBSRC Industrial Strategy Challenge |
| Biotechnology and Biological Sciences Research Council | |
| Funder project or code | Tailoring Plant Metabolism ISP |
| Understanding the role of lipases in controlling seed storage oil composition | |
| Tailoring Plant Metabolism (TPM) - Work package 1 (WP1) - High value lipids for health and industry | |
| Publisher's version | Copyright license CC BY |
| Accepted author manuscript | Copyright license CC BY |
| Supplemental file | Copyright license CC BY |
| Output status | Published |
| Publication dates | |
| Online | 26 Nov 2018 |
| Publication process dates | |
| Accepted | 26 Oct 2018 |
| Publisher | Nature Publishing Group |
| ISSN | 2045-2322 |
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Spatiotemporal expression patterns of wheat amino acid transporters reveal their putative roles in nitrogen transport and responses to abiotic stress
A - Papers appearing in refereed journals
Wan, Y., King, R., Mitchell, R. A. C., Hassani-Pak, K. and Hawkesford, M. J. 2017. Spatiotemporal expression patterns of wheat amino acid transporters reveal their putative roles in nitrogen transport and responses to abiotic stress. Scientific Reports. 7 (1), p. 5461. https://doi.org/10.1038/s41598-017-04473-3
Development of a statistical crop model to explain the relationship between seed yield and phenotypic diversity within the Brassica napus genepool
A - Papers appearing in refereed journals
Bennett, E., Brignell, C., Carion, P. W. C., Cook, S. M., Eastmond, P. J., Teakle, G., Hammond, J. P., Love, C., King, G. J., Roberts, J. A. and Wagstaff, C. 2017. Development of a statistical crop model to explain the relationship between seed yield and phenotypic diversity within the Brassica napus genepool. Agronomy. 7 (2), p. 31. https://doi.org/10.3390/agronomy7020031
Fatty acids in arbuscular mycorrhizal fungi are synthesized by the host plant
A - Papers appearing in refereed journals
Luginbuehl, L. H., Menard, G. N., Kurup, S., Van-Erp, H., Radhakrishnan, G. V., Breakspear, A., Oldroyd, G. E. D. and Eastmond, P. J. 2017. Fatty acids in arbuscular mycorrhizal fungi are synthesized by the host plant. Science - AAAS. 356 (6343), pp. 1175-1178. https://doi.org/10.1126/science.aan0081
Awake1, an ABC-type transporter, reveals an essential role for Suberin in the control of seed dormancy
A - Papers appearing in refereed journals
Fedi, F., O’neill, C. M., Menard, G., Trick, M., Dechirico, S., Corbineau, F., Bailly, C., Eastmond, P. J. and Penfield, S. 2017. Awake1, an ABC-type transporter, reveals an essential role for Suberin in the control of seed dormancy. Plant Physiology. 174 (1), pp. 276-283. https://doi.org/10.1104/pp.16.01556
Genome wide analysis of fatty acid desaturation and its response to temperature
A - Papers appearing in refereed journals
Menard, G. N., Martin-Moreno, J., Bryant, F. M., Munoz-Azcarate, O., Kelly, A. A., Hassani-Pak, K., Kurup, S. and Eastmond, P. J. 2017. Genome wide analysis of fatty acid desaturation and its response to temperature. Plant Physiology. 173 (3), pp. 1594-1605. https://doi.org/10.1104/pp.16.01907
Cyclin-dependent kinase activity enhances phosphatidylcholine biosynthesis in Arabidopsis by repressing phosphatidic acid phosphohydrolase activity
A - Papers appearing in refereed journals
Craddock, C. P., Adams, N., Kroon, J. T. M., Bryant, F. M., Hussey, P. J., Kurup, S. and Eastmond, P. J. 2017. Cyclin-dependent kinase activity enhances phosphatidylcholine biosynthesis in Arabidopsis by repressing phosphatidic acid phosphohydrolase activity. The Plant Journal. 89 (1), pp. 3-14. https://doi.org/10.1111/tpj.13321
KnetMiner - An integrated data platform for gene mining and biological knowledge discovery
F - Theses
Hassani-Pak, K. 2017. KnetMiner - An integrated data platform for gene mining and biological knowledge discovery. F - Theses Rothamsted Research Computational and Analytical Sciences
Developing integrated crop knowledge networks to advance candidate gene discovery
A - Papers appearing in refereed journals
Hassani-Pak, K., Castellote, M., Esch, M., Hindle, M. M., Lysenko, A., Taubert, J. and Rawlings, C. J. 2016. Developing integrated crop knowledge networks to advance candidate gene discovery. Applied and Translational Genomics. 11 (December), pp. 18-26. https://doi.org/10.1016/j.atg.2016.10.003
Insecticide resistance mediated by an exon skipping event.
A - Papers appearing in refereed journals
Berger, M., Puinean, A. M., Randall, E., Zimmer, C. T., Silva, W. M., Bielza, P., Field, L. M., Hughes, D. J., Mellor, I., Hassani-Pak, K., Siqueira, H. A. A., Williamson, M. S. and Bass, C. 2016. Insecticide resistance mediated by an exon skipping event. Molecular Ecology. 25 (22), pp. 5692-5704. https://doi.org/10.1111/mec.13882
ACYL-ACYL CARRIER PROTEIN DESATURASE2 and 3 are responsible for making omega-7 fatty acids in the Arabidopsis aleurone
A - Papers appearing in refereed journals
Bryant, F. M., Munoz-Azcarate, O., Kelly, A. A., Beaudoin, F., Kurup, S. and Eastmond, P. J. 2016. ACYL-ACYL CARRIER PROTEIN DESATURASE2 and 3 are responsible for making omega-7 fatty acids in the Arabidopsis aleurone. Plant Physiology. 172 (1), pp. 154-162. https://doi.org/10.1104/pp.16.00836
Expression of castor LPAT2 enhances ricinoleic acid content at the sn-2 position of triacylglycerols in lesquerella seed
A - Papers appearing in refereed journals
Chen, G. Q., Van-Erp, H., Martin-Moreno, J., Johnson, K., Morales, E., Browse, J., Eastmond, P. J. and Lin, J-T. 2016. Expression of castor LPAT2 enhances ricinoleic acid content at the sn-2 position of triacylglycerols in lesquerella seed. International Journal Of Molecular Sciences. 17 (4), p. 507. https://doi.org/10.3390/ijms17040507
Growing innovations for the bioeconomy
A - Papers appearing in refereed journals
Karp, A., Beale, M. H., Beaudoin, F., Eastmond, P. J., Neal, A. L., Shield, I. F., Townsend, B. J. and Dobermann, A. 2015. Growing innovations for the bioeconomy. Nature Plants. 1, p. 15193. https://doi.org/10.1038/nplants.2015.193
Mutation scanning in wheat by exon capture and next-generation sequencing
A - Papers appearing in refereed journals
King, R., Bird, N., Ramirez-Gonzalez, R., Coghill, J. A., Patil, A., Hassani-Pak, K., Uauy, C. and Phillips, A. L. 2015. Mutation scanning in wheat by exon capture and next-generation sequencing. PLOS ONE. 10, p. e0137549. https://doi.org/10.1371/journal.pone.0137549
Regulation of endomembrane biogenesis in arabidopsis by phospatidic acid hydrolase
A - Papers appearing in refereed journals
Craddock, C. P., Adams, N., Bryant, F. M., Kurup, S. and Eastmond, P. J. 2015. Regulation of endomembrane biogenesis in arabidopsis by phospatidic acid hydrolase. Plant Signaling and Behavior. 10, p. e1065367. https://doi.org/10.1080/15592324.2015.1065367
The completed genome sequence of the pathogenic ascomycete fungus Fusarium graminearum
A - Papers appearing in refereed journals
King, R., Urban, M., Hammond-Kosack, M. C. U., Hassani-Pak, K. and Hammond-Kosack, K. E. 2015. The completed genome sequence of the pathogenic ascomycete fungus Fusarium graminearum. BMC Genomics. 16, p. 544. https://doi.org/10.1186/s12864-015-1756-1
Whole-genome analysis of Fusarium graminearum insertional mutants identifies virulence associated genes and unmasks untagged chromosomal deletions
A - Papers appearing in refereed journals
Urban, M., King, R., Hassani-Pak, K. and Hammond-Kosack, K. E. 2015. Whole-genome analysis of Fusarium graminearum insertional mutants identifies virulence associated genes and unmasks untagged chromosomal deletions. BMC Genomics. 16, p. 261. https://doi.org/10.1186/s12864-015-1412-9
Arabidopsis uses two gluconeogenic gateways for organic acids to fuel seedling establishment
A - Papers appearing in refereed journals
Eastmond, P. J., Astley, H. M., Parsley, K., Aubry, S., Williams, B. P., Menard, G. N., Craddock, C. P., Nunes-Nesi, A., Fernie, A. R. and Hibberd, J. M. 2015. Arabidopsis uses two gluconeogenic gateways for organic acids to fuel seedling establishment. Nature Communications. 6, p. 6659. https://doi.org/10.1038/ncomms7659
PHOSPHATIDIC ACID PHOSPHOHYDROLASE regulates phosphatidylcholine biosynthesis in Arabidopsis by phosphatidic acid-mediated activation of CTP: PHOSPHOCHOLINE CYTIDYLYLTRANSFERASE activity
A - Papers appearing in refereed journals
Craddock, C. P., Adams, N., Bryant, F. M., Kurup, S. and Eastmond, P. J. 2015. PHOSPHATIDIC ACID PHOSPHOHYDROLASE regulates phosphatidylcholine biosynthesis in Arabidopsis by phosphatidic acid-mediated activation of CTP: PHOSPHOCHOLINE CYTIDYLYLTRANSFERASE activity. The Plant Cell. 27, pp. 1251-1264. https://doi.org/10.1105/tpc.15.00037
Transcriptome and metabolite profiling of the infection cycle of Zymoseptoria tritici on wheat reveals a biphasic interaction with plant immunity involving differential pathogen chromosomal contributions and a variation on the hemibiotrophic lifestyle definition
A - Papers appearing in refereed journals
Rudd, J. J., Kanyuka, K., Hassani-Pak, K., Derbyshire, M., Andongabo, A. E., Devonshire, J., Lysenko, A., Saqi, M., Desai, N. M., Powers, S. J., Hooper, J., Ambroso, L., Bharti, A., Farmer, A., Hammond-Kosack, K. E., Dietrich, R. A. and Courbot, M. 2015. Transcriptome and metabolite profiling of the infection cycle of Zymoseptoria tritici on wheat reveals a biphasic interaction with plant immunity involving differential pathogen chromosomal contributions and a variation on the hemibiotrophic lifestyle definition. Plant Physiology. 167 (3), pp. 1158-1185. https://doi.org/10.1104/pp.114.255927
Seed storage reserve analysis
A - Papers appearing in refereed journals
Erp, H., Menard, G. and Eastmond, P. J. 2014. Seed storage reserve analysis. Bio-protocol. 4 (20), p. e1263. https://doi.org/10.21769/bioprotoc.1263
Multigene engineering of triacylglycerol metabolism boosts seed oil content in Arabidopsis
A - Papers appearing in refereed journals
Van-Erp, H., Kelly, A. A., Menard, G. N. and Eastmond, P. J. 2014. Multigene engineering of triacylglycerol metabolism boosts seed oil content in Arabidopsis. Plant Physiology. 165, pp. 30-36. https://doi.org/10.1104/pp.114.236430
A tale of one fungal pathogen of wheat: how it evades host surveillance and then hijacks host defences
C2 - Non-edited contributions to conferences
Kanyuka, K., Lysenko, A., Saqi, M., Derbyshire, M., Andongabo, A. E., Hassani-Pak, K., Hammond-Kosack, K. E., Courbot, M. and Rudd, J. J. 2014. A tale of one fungal pathogen of wheat: how it evades host surveillance and then hijacks host defences. Abstracts 16th International Congress on Molecular Plant-Microbe Interactions (IS-MPMI 2014), Rhodes, 6-10 July 2014 . pp. P069
Identification and analysis of differentially expressed genes in the wheat- Zymoseptoria tritici interaction
C2 - Non-edited contributions to conferences
Kanyuka, K., Andongabo, A. E., Hassani-Pak, K., Powers, S. J., Lysenko, A., Saqi, M., Derbyshire, M., Lee, W-S., Hammond-Kosack, K. E., Courbot, M. and Rudd, J. J. 2014. Identification and analysis of differentially expressed genes in the wheat- Zymoseptoria tritici interaction. Abstracts Plant Genomics Congress, London, 12-13 May 2014 .
A query suggestion workflow for life science IR-systems
A - Papers appearing in refereed journals
Esch, M., Chen, J., Weise, S., Hassani-Pak, K., Scholz, U. and Lange, M. 2014. A query suggestion workflow for life science IR-systems. Journal of Integrative Bioinformatics. 11, p. 237. https://doi.org/10.2390/biecoll-jib-2014-237
The Arabidopsis thaliana RNA editing factor SLO2, which affects the mitochondrial electron transport chain, participates in multiple stress and hormone responses
A - Papers appearing in refereed journals
Zhu, Q., Dugardeyn, J., Zhang, C., Muhlenbock, P., Eastmond, P. J., Valcke, R., De Coninck, B., Oden, S., Karampelias, M., Cammue, B. P. A., Prinsen, E. and Van Der Straeten, D. 2014. The Arabidopsis thaliana RNA editing factor SLO2, which affects the mitochondrial electron transport chain, participates in multiple stress and hormone responses. Molecular Plant. 7 (2), pp. 290-310. https://doi.org/10.1093/mp/sst102
Biomass oil crops: more than a pipe dream?
A - Papers appearing in refereed journals
Eastmond, P. J. 2014. Biomass oil crops: more than a pipe dream? Biofuels. 5 (2), pp. 99-101. https://doi.org/10.4155/BFS.13.77
Secondary cell wall composition and candidate gene expression in developing willow (Salix purpurea) stems
A - Papers appearing in refereed journals
Wan, Y., Gritsch, C., Tryfona, T., Ray, M. J., Andongabo, A. E., Hassani-Pak, K., Jones, H. D., Dupree, P., Karp, A., Shewry, P. R. and Mitchell, R. A. C. 2014. Secondary cell wall composition and candidate gene expression in developing willow (Salix purpurea) stems. Planta. 239, pp. 1041-1053. https://doi.org/10.1007/s00425-014-2034-1
Ondex Web: web-based visualization and exploration of heterogeneous biological networks
A - Papers appearing in refereed journals
Taubert, J., Hassani-Pak, K., Castells-Brooke, N. I. D. and Rawlings, C. J. 2014. Ondex Web: web-based visualization and exploration of heterogeneous biological networks. Bioinformatics. 30, pp. 1034-1035. https://doi.org/10.1093/bioinformatics/btt740
bZIP67 regulates the omega-3 fatty acid content of Arabidopsis seed oil by activating FATTY ACID DESATURASE3
A - Papers appearing in refereed journals
Mendes, A., Kelly, A. A., Van-Erp, H., Shaw, E., Powers, S. J., Kurup, S. and Eastmond, P. J. 2013. bZIP67 regulates the omega-3 fatty acid content of Arabidopsis seed oil by activating FATTY ACID DESATURASE3. The Plant Cell. 25, pp. 3104-3116. https://doi.org/10.1105/tpc.113.116343
The SUGAR-DEPENDENT1 lipase limits triacyglycerol accumulation in vegetative tissues of Arabidopsis
A - Papers appearing in refereed journals
Kelly, A. A., Van-Erp, H., Quettier, A-L., Shaw, E., Menard, G. N., Kurup, S. and Eastmond, P. J. 2013. The SUGAR-DEPENDENT1 lipase limits triacyglycerol accumulation in vegetative tissues of Arabidopsis. Plant Physiology. 162, pp. 1282-1289. https://doi.org/10.1104/pp.113.219840
Differential defence response due to jasmonate seed treatment in cowpea and tomato against root-knot and potato cyst nematodes
A - Papers appearing in refereed journals
Pankaj, Muttucumaru, N., Powers, S. J., Gaur, H. S., Kurup, S. and Curtis, R. H. C. 2013. Differential defence response due to jasmonate seed treatment in cowpea and tomato against root-knot and potato cyst nematodes. Nematology. 15, pp. 15-21. https://doi.org/10.1163/156854112X641754
Suppression of the SUGAR-DEPENDENT1 triacylglycerol lipase family during seed development enhances oil yield in oilseed rape (Brassica napus L.)
A - Papers appearing in refereed journals
Kelly, A. A., Shaw, E., Powers, S. J., Kurup, S. and Eastmond, P. J. 2013. Suppression of the SUGAR-DEPENDENT1 triacylglycerol lipase family during seed development enhances oil yield in oilseed rape (Brassica napus L.). Plant Biotechnology Journal. 11 (3), pp. 355-361. https://doi.org/10.1111/pbi.12021
The modification of plant oil composition via metabolic engineering - better nutrition by design
A - Papers appearing in refereed journals
Haslam, R. P., Ruiz-Lopez, N., Eastmond, P. J., Moloney, M., Sayanova, O. V. and Napier, J. A. 2013. The modification of plant oil composition via metabolic engineering - better nutrition by design. Plant Biotechnology Journal. 11 (2), pp. 157-168. https://doi.org/10.1111/pbi.12012
Molecular characterization of the fatty alcohol oxidation pathway for wax-ester mobilization in germinated jojoba seeds
A - Papers appearing in refereed journals
Rajangam, A. S., Gidda, S. K., Craddock, C., Mullen, R. T., Dyer, J. M. and Eastmond, P. J. 2013. Molecular characterization of the fatty alcohol oxidation pathway for wax-ester mobilization in germinated jojoba seeds. Plant Physiology. 161, pp. 72-80. https://doi.org/10.1104/pp.112.208264
Promoter variation and transcript divergence in Brassicaceae lineages of FLOWERING LOCUST T
A - Papers appearing in refereed journals
Wang, J., Hopkins, C. J., Hou, J., Zou, X., Wang, C., Long, Y., Kurup, S., King, G. J. and Meng, J. 2012. Promoter variation and transcript divergence in Brassicaceae lineages of FLOWERING LOCUST T. PLOS ONE. 7, p. e47127. https://doi.org/10.1371/journal.pone.0047127
Parental genome imbalance in Brassica oleracea causes asymmetric triploid block
A - Papers appearing in refereed journals
Stoute, A. I., Varenko, V., King, G. J., Scott, R. J. and Kurup, S. 2012. Parental genome imbalance in Brassica oleracea causes asymmetric triploid block. The Plant Journal. 71 (3), pp. 503-516. https://doi.org/10.1111/j.1365-313X.2012.05015.x
A hypomethylated population of Brassica rapa for forward and reverse epi-genetics
A - Papers appearing in refereed journals
Amoah, S., Kurup, S., Rodriguez Lopez, C. M., Welham, S. J., Powers, S. J., Hopkins, C. J., Wilkinson, M. J. and King, G. J. 2012. A hypomethylated population of Brassica rapa for forward and reverse epi-genetics. BMC Plant Biology. 12, p. 193 (17pp). https://doi.org/10.1186/1471-2229-12-193
SLO2, a mitochondrial pentatricopeptide repeat protein affecting several RNA editing sites, is required for energy metabolism
A - Papers appearing in refereed journals
Zhu, Q., Dugardeyn, J., Zhang, C., Takenaka, M., Kuhn, K., Craddock, C., Smalle, J., Karampelias, M., Denecke, J., Peters, J., Gerats, T., Brennicke, A., Eastmond, P. J., Meyer, E. H. and Van Der Straeten, D. 2012. SLO2, a mitochondrial pentatricopeptide repeat protein affecting several RNA editing sites, is required for energy metabolism. The Plant Journal. 71 (5), pp. 836-849. https://doi.org/10.1111/j.1365-313X.2012.05036.x
A common signaling process that promotes mycorrhizal and oomycete colonization of plants
A - Papers appearing in refereed journals
Wang, E., Schornack, S., Marsh, J. F., Gobbato, E., Schwessinger, B., Eastmond, P. J., Schultze, M., Kamoun, S. and Oldroyd, G. E. D. 2012. A common signaling process that promotes mycorrhizal and oomycete colonization of plants. Current Biology. 22 (23), pp. 2242-2246. https://doi.org/10.1016/j.cub.2012.09.043
Seed storage oil catabolism: a story of give and take
A - Papers appearing in refereed journals
Theodoulou, F. L. and Eastmond, P. J. 2012. Seed storage oil catabolism: a story of give and take. Current Opinion in Plant Biology. 15 (3), pp. 322-328. https://doi.org/10.1016/j.pbi.2012.03.017
Seed colour loci, homoeology and linkage groups of the C genome chromosomes revealed in Brassica rapa-B. oleracea monosomic alien addition lines
A - Papers appearing in refereed journals
Heneen, W. K., Geleta, M., Brismar, K., Xiong, Z., Pires, J. C., Hasterok, R., Stoute, A. I., Scott, R. J., King, G. J. and Kurup, S. 2012. Seed colour loci, homoeology and linkage groups of the C genome chromosomes revealed in Brassica rapa-B. oleracea monosomic alien addition lines. Annals of Botany. 109, pp. 1227-1242. https://doi.org/10.1093/aob/mcs052
Distribution of calcium (Ca) and magnesium (Mg) in the leaves of Brassica rapa under varying exogenous Ca and Mg supply
A - Papers appearing in refereed journals
Rios, J. J., O Lochlainn, S., Devonshire, J., Graham, N. S., Hammond, J. P., King, G. J., White, P. J., Kurup, S. and Broadley, M. R. 2012. Distribution of calcium (Ca) and magnesium (Mg) in the leaves of Brassica rapa under varying exogenous Ca and Mg supply. Annals of Botany. 109, pp. 1081-1089. https://doi.org/10.1093/aob/mcs029
Assigning Brassica microsatellite markers to the nine C-genome chromosomes using Brassica rapa var. tricularis-B.oleracea var. alboglabra monosomic alien addition lines
A - Papers appearing in refereed journals
Geleta, M., Heneen, W. K., Stoute, A. I., Muttucumaru, N., Scott, R. J., King, G. J., Kurup, S. and Bryngelsson, T. 2012. Assigning Brassica microsatellite markers to the nine C-genome chromosomes using Brassica rapa var. tricularis-B.oleracea var. alboglabra monosomic alien addition lines. Theoretical and Applied Genetics. 125, pp. 455-466. https://doi.org/10.1007/s00122-012-1845-3
Gaining confidence in cross-species annotation transfer: from simple molecular function to complex phenotypic traits
C1 - Edited contributions to conferences/learned societies
Defoin-Platel, M., Hassani-Pak, K. and Rawlings, C. J. 2011. Gaining confidence in cross-species annotation transfer: from simple molecular function to complex phenotypic traits. Systems Approaches to Crop Improvement. Aspects of Applied Biology 107 . Association of Applied Biologists (AAB) Wellesbourne Warwick . pp. 79-87
Universal endogenous gene controls for bisulphite conversion in analysis of plant DNA methylation
A - Papers appearing in refereed journals
Wang, J., Wang, C., Long, Y., Hopkins, C., Kurup, S., Liu, K., King, G. J. and Meng, J. 2011. Universal endogenous gene controls for bisulphite conversion in analysis of plant DNA methylation. Plant Methods. 7, p. 39 (7pp). https://doi.org/10.1186/1746-4811-7-39
High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex crop genomes
A - Papers appearing in refereed journals
O Lochlainn, S., Amoah, S., Graham, N. S., Alamer, K., Rios, J. J., Kurup, S., Stoute, A., Hammond, J. P., Ostergaard, L., King, G. J., White, P. J. and Broadley, M. R. 2011. High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex crop genomes. Plant Methods. 7, p. 43 (9pp). https://doi.org/10.1186/1746-4811-7-43
Assessing the functional coherence of modules found in multiple-evidence networks from Arabidopsis
A - Papers appearing in refereed journals
Lysenko, A., Defoin-Platel, M., Hassani-Pak, K., Taubert, J., Hodgman, C., Rawlings, C. J. and Saqi, M. 2011. Assessing the functional coherence of modules found in multiple-evidence networks from Arabidopsis. BMC Bioinformatics. 12, p. 203 (14pp). https://doi.org/10.1186/1471-2105-12-203
Exploring and exploiting epigenetic variation in crops
A - Papers appearing in refereed journals
King, G. J., Amoah, S. and Kurup, S. 2010. Exploring and exploiting epigenetic variation in crops. Genome. 53, pp. 856-868. https://doi.org/10.1139/G10-059
Enhancing data integration with text analysis to find proteins implicated in plant stress response
A - Papers appearing in refereed journals
Hassani-Pak, K., Legaie, R., Canevet, C., Van Den Berg, H. A., Moore, J. D. and Rawlings, C. J. 2010. Enhancing data integration with text analysis to find proteins implicated in plant stress response. Journal of Integrative Bioinformatics. 7, p. 121 (13pp). https://doi.org/10.2390/biecoll-jib-2010-121
Using cell-lineage tagging to decipher early differentiation and development in wheat endosperm
C1 - Edited contributions to conferences/learned societies
Freeman, J., Kurup, S., Huttly, A. K. and Shewry, P. R. 2008. Using cell-lineage tagging to decipher early differentiation and development in wheat endosperm. Molinacano, J. L. (ed.) Cereal Science and Technology for Feeding Ten Billion People: Genomics Era and Beyond. Proceedings of the Meeting of the Eucarpia Cereal Section, Lleida (Spain), 13-17 November 2006 . Options Mediterraneennes, Series A, No 81. pp. 453-455
Nematicidal effects of cysteine proteinases and methods of use thereof to treat nematode infestation. International patent application WO 2008/087555
H2 - Patents applied for
Curtis, R. H. C., Buttle, D., Behnke, J., Duce, I., Shewry, P. R., Kurup, S. and Kerry, B. R. 2008. Nematicidal effects of cysteine proteinases and methods of use thereof to treat nematode infestation. International patent application WO 2008/087555.
Graph-based sequence annotation using a data integration approach
A - Papers appearing in refereed journals
Pesch, R., Lysenko, A., Hindle, M. M., Hassani-Pak, K., Thiele, R., Rawlings, C. J., Kohler, J. and Taubert, J. 2008. Graph-based sequence annotation using a data integration approach. Journal of Integrative Bioinformatics. 5(2):94, p. 15pp. https://doi.org/10.2390/biecoll-jib-2008-94
Signalling mechanisms in the regulation of vacuolar ion release in guard cells
A - Papers appearing in refereed journals
Macrobbie, E. A. C. and Kurup, S. 2007. Signalling mechanisms in the regulation of vacuolar ion release in guard cells. New Phytologist. 175, pp. 630-640. https://doi.org/10.1111/j.1469-8137.2007.02131.x
Using cell-lineage tagging to decipher early differentiation and development in wheat endosperm
C2 - Non-edited contributions to conferences
Freeman, J., Kurup, S., Huttly, A. K. and Shewry, P. R. 2007. Using cell-lineage tagging to decipher early differentiation and development in wheat endosperm. Abstracts 4th UK Small Grain Cereals Genetic and Genomics Workshop: From Genes to Traits, Cambridge, 14-16 March 2007 .
Transactivated and chemically inducible gene expression in plants
A - Papers appearing in refereed journals
Moore, I., Samalova, M. and Kurup, S. 2006. Transactivated and chemically inducible gene expression in plants. The Plant Journal. 45 (4), pp. 651-683. https://doi.org/10.1111/j.1365-313X.2006.02660.x
Using cell lineage tagging to decipher early differentiation and development in wheat endosperm
C2 - Non-edited contributions to conferences
Freeman, J., Kurup, S. and Shewry, P. R. 2005. Using cell lineage tagging to decipher early differentiation and development in wheat endosperm. Abstracts 7th International Wheat Conference, Mar del Plata, 27 November - 2 December 2005 .
Gene expression construct: US patent application 20050132432
H2 - Patents applied for
Haseloff, J., Bauch, M., Boisnard-Lorig, C., Hodge, S., Laplaze, L., Runions, J. and Kurup, S. 2005. Gene expression construct: US patent application 20050132432.
Marking cell lineages in living tissue
A - Papers appearing in refereed journals
Kurup, S., Runions, J., Kohler, U., Laplaze, L., Hodge, S. and Haseloff, J. 2005. Marking cell lineages in living tissue. The Plant Journal. 42 (3), pp. 444-453. https://doi.org/10.1111/j.1365-313X.2005.02386.x
Control of germination and lipid mobilization by COMATOSE , the Arabidopsis homologue of human ALDP
A - Papers appearing in refereed journals
Footitt, S., Slocombe, S. P., Larner, V., Kurup, S., Wu, Y., Larson, T., Graham, I., Baker, A. and Holdsworth, M. J. 2002. Control of germination and lipid mobilization by COMATOSE , the Arabidopsis homologue of human ALDP. Embo Journal. 21, pp. 2912-2922.
Genetic control mechanisms regulating the initiation of germination
A - Papers appearing in refereed journals
Holdsworth, M. J., Lenton, J. R., Flintham, J., Gale, M., Kurup, S., Mckibbin, R. S., Bailey, P., Larner, V. and Russell, L. 2001. Genetic control mechanisms regulating the initiation of germination. Journal of Plant Physiology. 158, pp. 439-445.
Identification and analysis of proteins that interact with the Avena fatua homologue of the maize transcription factor VIVIPAROUS 1
A - Papers appearing in refereed journals
Jones, H. D., Kurup, S., Peters, N. C. B and Holdsworth, M. J. 2000. Identification and analysis of proteins that interact with the Avena fatua homologue of the maize transcription factor VIVIPAROUS 1. The Plant Journal. 21 (2), pp. 133-142. https://doi.org/10.1046/j.1365-313x.2000.00662.x
AB13 emerges from the seed
A - Papers appearing in refereed journals
Rohde, A., Kurup, S. and Holdsworth, M. J. 2000. AB13 emerges from the seed. Trends in Plant Science. 5, pp. 418-419.
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