Nitrogen and fatty acid rumen metabolism in cattle offered high or low polyphenol oxidase red clover silage

A - Papers appearing in refereed journals

Lee, M. R. F., Fychan, R., Tweed, J. K. S., Gordon, N., Theobald V., Yadav, R. and Marshall, A. 2018. Nitrogen and fatty acid rumen metabolism in cattle offered high or low polyphenol oxidase red clover silage. Animal. pp. 1-12.

AuthorsLee, M. R. F., Fychan, R., Tweed, J. K. S., Gordon, N., Theobald V., Yadav, R. and Marshall, A.
Abstract

Polyphenol oxidase (PPO) in red clover (RC) has been shown to reduce both lipolysis and proteolysis in silo and implicated (in vitro) in the rumen. However, all in vivo comparisons have compared RC with other forages, typically with lower levels of PPO, which brings in other confounding factors as to the cause for the greater protection of dietary nitrogen (N) and C18 polyunsaturated fatty acids (PUFA) on RC silage. This study compared two RC silages which when ensiled had contrasting PPO activities (RC+ and RC−) against a control of perennial ryegrass silage (PRG) to ascertain the effect of PPO activity on dietary N digestibility and PUFA biohydrogenation. Two studies
were performed the first to investigate rumen and duodenal flow with six Hereford ×Friesian steers, prepared with rumen and duodenal cannulae, and the second investigating whole tract N balance using six Holstein-Friesian non-lactating dairy cows. All diets were offered at a restricted level based on animal live weight with each experiment consisting of two 3 ×3 Latin squares using big bale silages ensiled in 2010 and 2011, respectively. For the first experiment digesta flow at the duodenum was estimated using a dual-phase marker system with ytterbium acetate and chromium ethylenediaminetetraacetic acid as particulate and liquid phase markers, respectively. Total N intake was higher on the RC silages in both experiments and higher on RC− than RC+. Rumen ammonia-N reflected intake with ammonia-N per unit of N intake lower on RC+ than RC−. Microbial N duodenal flow was comparable across all silage diets with nonmicrobial
N higher on RC than the PRG with no difference between RC+ and RC−, even when reported on a N intake basis. C18 PUFA
biohydrogenation was lower on RC silage diets than PRG but with no difference between RC+ and RC−. The N balance trial showed a greater retention of N on RC+ over RC−; however, this response is likely related to the difference in N intake over any PPO driven protection. The lack of difference between RC silages, despite contrasting levels of PPO, may reflect a similar level of protein-boundphenol complexing determined in each RC silage. Previously this complexing has been associated with PPOs protection mechanism; however, this study has shown that protection is not related to total PPO activity.

KeywordsPlant enzymes; Forage legumes; Trifolium pratense; C18 polyunsaturated fatty acid biohydrogenation; Nitrogen use efficiency
Year of Publication2018
JournalAnimal
Journal citationpp. 1-12
Digital Object Identifier (DOI)doi:10.1017/S1751731118003294
PubMed ID30565534
Open accessPublished as ‘gold’ (paid) open access
FunderDepartment of Environment, Food and Rural Affairs
Biotechnology and Biological Sciences Research Council
Funder project or codeS2N - Soil to Nutrition - Work package 2 (WP2) - Adaptive management systems for improved efficiency and nutritional quality
Publisher's version
Accepted author manuscript
Output statusE-publication ahead of print
Publication dates
Online19 Dec 2018
Publication process dates
Accepted18 Oct 2018
PublisherCambridge University Press (CUP)
Copyright licenseCC BY
ISSN1751-7311

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