Glyphosate resistance and EPSPS gene duplication: convergent evolution in multiple plant species

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Patterson, E.L., Pettinga, D.J., Ravet, K., Neve, P. and Gaines, T.A. 2018. Glyphosate resistance and EPSPS gene duplication: convergent evolution in multiple plant species. Journal Of Heredity. 109 (2), pp. 117-125.

AuthorsPatterson, E.L., Pettinga, D.J., Ravet, K., Neve, P. and Gaines, T.A.
Abstract

One of the increasingly widespread mechanisms of resistance to the herbicide glyphosate is copy number variation (CNV) of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene. EPSPS gene duplication has been reported in 8 weed species, ranging from 3 to 5 extra copies to more than 150 extra copies. In the case of Palmer amaranth (Amaranthus palmeri), a section of >300 kb containing EPSPS and many other genes has been replicated and inserted at new loci throughout the genome, resulting in significant increase in total genome size. The replicated sequence contains several classes of mobile genetic elements including helitrons, raising the intriguing possibility of extra-chromosomal replication of the EPSPS-containing sequence. In kochia (Kochia scoparia), from 3 to more than 10 extra EPSPS copies are arranged as a tandem gene duplication at one locus. In the remaining 6 weed species that exhibit EPSPS gene duplication, little is known about the underlying mechanisms of gene duplication or their entire sequence. There is mounting evidence that adaptive gene amplification is an important mode of evolution in the face of intense human-mediated selection pressure. The convergent evolution of CNVs for glyphosate resistance in weeds, through at least 2 different mechanisms, may be indicative of a more generalimportance for this mechanism of adaptation in plants. CNVs warrant further investigation across plant functional genomics for adaptation to biotic and abiotic stresses, particularly for adaptive
evolution on rapid time scales.

Keywordsgene amplification; gene copy number variation; Molecular adaptation and selection
Year of Publication2018
JournalJournal Of Heredity
Journal citation109 (2), pp. 117-125
Digital Object Identifier (DOI)doi:10.1093/jhered/esx087
PubMed ID29040588
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeUnited States: Harnessing next-generation sequencing technologies for eco-evolutionary studies of herbicide resistance and weed biology
Output statusPublished
Publication dates
Online10 Oct 2017
Publication process dates
Accepted02 Oct 2017
PublisherOxford Univ Press Inc
Copyright licensePublisher copyright
ISSN0022-1503

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