Genetic transformation in two potato cultivars with T-DNA from disarmed Agrobacterium

A - Papers appearing in refereed journals

Ooms, G., Burrell, M. M., Karp, A., Bevan, M. and Hille, J. 1987. Genetic transformation in two potato cultivars with T-DNA from disarmed Agrobacterium. Theoretical and Applied Genetics. 73 (5), pp. 744-750.

AuthorsOoms, G., Burrell, M. M., Karp, A., Bevan, M. and Hille, J.
Abstract

Derivatives of potato (Solanum tuberosum cv.'s ‘Maris Bard’ and ‘Desiree’) transformed with disarmed T-DNA from genetically engineered Agrobacterium tumefaciens strains were isolated. The transformed plants were recovered from shoot-forming tumours induced by infection of wounds with mixedcultures of shoot-inducing A. tumefaciens strains T37 and either Agrobacterium strain LBA1834(pRAL1834), (Hille et al. 1983) or LBA4404(pBIN6; pRAL4404), (Bevan 1984). Two small-scale feasibility experiments gave at least four ‘Maris Bard’ plants transformed with pRAL1834 T-DNA and two ‘Desiree’ plants with pBIN6 T-DNA. The transformed ‘Maris Bard’ plants were morphologically abnormal and highly aneuploid. This was probably an unfortunate side-effect of a tissue culture-step introduced to promote the efficiency of shoot regeneration. The transformed ‘Desiree’ plants, in contrast, were isolated without promoting additional shoot-growth. They were morphologically normal, contained 47 and the euploid 48 chromosomes per cell respectively and had improved growth on media containing kanamycin. 

Year of Publication1987
JournalTheoretical and Applied Genetics
Journal citation73 (5), pp. 744-750
Digital Object Identifier (DOI)doi:10.1007/BF00260785
PubMed ID24241200
Open accessPublished as non-open access
PublisherSpringer Nature
ISSN0040-5752

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