A - Papers appearing in refereed journals
Ooms, G., Burrell, M. M., Karp, A., Bevan, M. and Hille, J. 1987. Genetic transformation in two potato cultivars with T-DNA from disarmed Agrobacterium. Theoretical and Applied Genetics. 73 (5), pp. 744-750.
|Authors||Ooms, G., Burrell, M. M., Karp, A., Bevan, M. and Hille, J.|
Derivatives of potato (Solanum tuberosum cv.'s ‘Maris Bard’ and ‘Desiree’) transformed with disarmed T-DNA from genetically engineered Agrobacterium tumefaciens strains were isolated. The transformed plants were recovered from shoot-forming tumours induced by infection of wounds with mixedcultures of shoot-inducing A. tumefaciens strains T37 and either Agrobacterium strain LBA1834(pRAL1834), (Hille et al. 1983) or LBA4404(pBIN6; pRAL4404), (Bevan 1984). Two small-scale feasibility experiments gave at least four ‘Maris Bard’ plants transformed with pRAL1834 T-DNA and two ‘Desiree’ plants with pBIN6 T-DNA. The transformed ‘Maris Bard’ plants were morphologically abnormal and highly aneuploid. This was probably an unfortunate side-effect of a tissue culture-step introduced to promote the efficiency of shoot regeneration. The transformed ‘Desiree’ plants, in contrast, were isolated without promoting additional shoot-growth. They were morphologically normal, contained 47 and the euploid 48 chromosomes per cell respectively and had improved growth on media containing kanamycin.
|Year of Publication||1987|
|Journal||Theoretical and Applied Genetics|
|Journal citation||73 (5), pp. 744-750|
|Digital Object Identifier (DOI)||doi:10.1007/BF00260785|
|Open access||Published as non-open access|
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