Functional expression of a plant plasma membrane transporter in Xenopus oocytes

C2 - Non-edited contributions to conferences

Boorer, K. J., Forde, B. G., Leigh, R. A. and Miller, A. J. 1992. Functional expression of a plant plasma membrane transporter in Xenopus oocytes. Proceedings 9th International Workshop on Plant Membrane Biology, Monterey, July 1992 . Wiley. doi:10.1016/0014-5793(92)80431-F

AuthorsBoorer, K. J., Forde, B. G., Leigh, R. A. and Miller, A. J.
TypeC2 - Non-edited contributions to conferences
Abstract

A full-length cDNA clone for the H+/hexose co-transporter (STP1) from Arabidopsis thaliana has been transcribed in vitro and the mRNA injected into Xenopus oocytes. Under optimized conditions, oocytes injected with the STP1 mRNA accumulated 3-O-[methyl-C-14]glucose at rates of more than a 1000-fold greater than water-injected control oocytes. A hexose-elicited depolarization of the oocyte membrane potential was demonstrated, and uptake was shown to be stimulated by low external pH, confirming the activity of a H+/hexose co-transport system. This is the first example of the functional expression of a plant membrane transporter in oocytes.

KeywordsBiochemistry & Molecular Biology; Biophysics; Cell Biology
Year of Publication1992
Digital Object Identifier (DOI)doi:10.1016/0014-5793(92)80431-F
PubMed ID1633849
Journal citation(2), p. 23
PublisherWiley
Funder project or code114
212
ISSN00145793

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