A - Papers appearing in refereed journals
Gomes, E., Gordon-Weeks, R., Simon-Plas, F., Pugin, A., Milat, M-L., Leigh, R. A. and Blein, J-P. 1996. Cercospora beticola toxins. Part XVII. The role of beticolin/Mg2+ complexes in their biological activity. Study of plasma membrane H+-ATPase, vacuolar H+-PPase, alkaline and acid phosphatases. Biochimica et Biophysica Acta (BBA) - Biomembranes. 1285 (1), pp. 38-46.
|Authors||Gomes, E., Gordon-Weeks, R., Simon-Plas, F., Pugin, A., Milat, M-L., Leigh, R. A. and Blein, J-P.|
Beticolin-1 and beticolin-2, yellow toxins produced by the phytopathogenic fungus Cercospora beticola, inhibit the plasma membrane H+-ATPase. Firstly, since beticolins are able to form complexes with Mg2+, the role of the beticolin/Mg2+ complexes in the inhibition of the plasma membrane proton pump has been investigated. Calculations indicate that beticolins could exist under several forms, in the H+-ATPase assay mixture, both free or complexed with Mg2+. However, the percentage inhibition of the H+-ATPase activity is correlated to the concentration of one single form of beticolin, the dimeric neutral complex Mg2H2B2, which appears to be the active form involved in the H+-ATPase inhibition. Secondly, since previous data suggested that beticolins could also be active against other Mg2+-dependent enzymes, we tested beticolin-1 on the vacoular H+-PPase, which requires Mg2+ as co-substrate, and on the alkaline and acid phosphatases, which do not use Mg2+ as co-substrate. Only vacuolar H+-PPase is sensitive to beticolin-1, which suggests that beticolins are specific to enzymes that use a complex of Mg2+ as the substrate. The same Mg2H2B2 complex which is responsible of the plasma membrane H+-ATPase inhibition appears to be also involved in the inhibition of the vacuolar H+-PPase.
|Keywords||Beticolin; H+- ATPase; H+- Pyrophosphatase; Inhibition; Magnesium ion complex|
|Year of Publication||1996|
|Journal||Biochimica et Biophysica Acta (BBA) - Biomembranes|
|Journal citation||1285 (1), pp. 38-46|
|Digital Object Identifier (DOI)||doi:10.1016/S0005-2736(96)00144-7|
|Open access||Published as bronze (free) open access|
|Funder project or code||212|
|13 Nov 1996|
|Publication process dates|
|Accepted||24 Jul 1996|
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