Cercospora beticola toxins. Part XVII. The role of beticolin/Mg2+ complexes in their biological activity. Study of plasma membrane H+-ATPase, vacuolar H+-PPase, alkaline and acid phosphatases

A - Papers appearing in refereed journals

Gomes, E., Gordon-Weeks, R., Simon-Plas, F., Pugin, A., Milat, M-L., Leigh, R. A. and Blein, J-P. 1996. Cercospora beticola toxins. Part XVII. The role of beticolin/Mg2+ complexes in their biological activity. Study of plasma membrane H+-ATPase, vacuolar H+-PPase, alkaline and acid phosphatases. Biochimica et Biophysica Acta (BBA) - Biomembranes. 1285 (1), pp. 38-46.

AuthorsGomes, E., Gordon-Weeks, R., Simon-Plas, F., Pugin, A., Milat, M-L., Leigh, R. A. and Blein, J-P.
Abstract

Beticolin-1 and beticolin-2, yellow toxins produced by the phytopathogenic fungus Cercospora beticola, inhibit the plasma membrane H+-ATPase. Firstly, since beticolins are able to form complexes with Mg2+, the role of the beticolin/Mg2+ complexes in the inhibition of the plasma membrane proton pump has been investigated. Calculations indicate that beticolins could exist under several forms, in the H+-ATPase assay mixture, both free or complexed with Mg2+. However, the percentage inhibition of the H+-ATPase activity is correlated to the concentration of one single form of beticolin, the dimeric neutral complex Mg2H2B2, which appears to be the active form involved in the H+-ATPase inhibition. Secondly, since previous data suggested that beticolins could also be active against other Mg2+-dependent enzymes, we tested beticolin-1 on the vacoular H+-PPase, which requires Mg2+ as co-substrate, and on the alkaline and acid phosphatases, which do not use Mg2+ as co-substrate. Only vacuolar H+-PPase is sensitive to beticolin-1, which suggests that beticolins are specific to enzymes that use a complex of Mg2+ as the substrate. The same Mg2H2B2 complex which is responsible of the plasma membrane H+-ATPase inhibition appears to be also involved in the inhibition of the vacuolar H+-PPase.

KeywordsBeticolin; H+- ATPase; H+- Pyrophosphatase; Inhibition; Magnesium ion complex
Year of Publication1996
JournalBiochimica et Biophysica Acta (BBA) - Biomembranes
Journal citation1285 (1), pp. 38-46
Digital Object Identifier (DOI)doi:10.1016/S0005-2736(96)00144-7
Open accessPublished as bronze (free) open access
Funder project or code212
442
Project: 011419
Publisher's version
Copyright license
Publisher copyright
Output statusPublished
Publication dates
Print13 Nov 1996
Publication process dates
Accepted24 Jul 1996
ISSN00052736
PublisherElsevier

Permalink - https://repository.rothamsted.ac.uk/item/8787q/cercospora-beticola-toxins-part-xvii-the-role-of-beticolin-mg2-complexes-in-their-biological-activity-study-of-plasma-membrane-h-atpase-vacuolar-h-ppase-alkaline-and-acid-phosphatases

9 total views
1 total downloads
0 views this month
0 downloads this month
Download files as zip