A - Papers appearing in refereed journals
Field, L. M., Crick, S. E. and Devonshire, A. L. 1996. Polymerase chain reaction-based identification of insecticide resistance genes and DNA methylation in the aphid Myzus persicae (Sulzer). Insect Molecular Biology. 5 (3), pp. 197-202.
|Authors||Field, L. M., Crick, S. E. and Devonshire, A. L.|
The ability of a peach-potato aphid (Myzus persicae) to resist insecticides can depend on both the presence of amplified esterase genes and on differences in their expression. Here we report a polymerase chain reaction-restriction enzyme (PCR-REN) technique which can detect the presence of amplified esterase genes and distinguish between the two possible types of amplified gene (E4 and FE4) and a homologous fragment from susceptible aphids. The technique is quick and sensitive enough to be used on a fraction of an aphid or an individual embryo. Furthermore, it can be combined with a pre-PCR digestion using a methylation-sensitive enzyme (Hpall) to determine whether or not the esterase genes contain 5-methylcytosine, the presence or absence of which is known to correlate with changes in gene expression.
|Keywords||Biochemistry & Molecular Biology; Entomology|
|Year of Publication||1996|
|Journal||Insect Molecular Biology|
|Journal citation||5 (3), pp. 197-202|
|Digital Object Identifier (DOI)||doi:10.1111/j.1365-2583.1996.tb00054.x|
|Open access||Published as non-open access|
|Funder project or code||235|
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