Polymerase chain reaction-based identification of insecticide resistance genes and DNA methylation in the aphid Myzus persicae (Sulzer)

A - Papers appearing in refereed journals

Field, L. M., Crick, S. E. and Devonshire, A. L. 1996. Polymerase chain reaction-based identification of insecticide resistance genes and DNA methylation in the aphid Myzus persicae (Sulzer). Insect Molecular Biology. 5 (3), pp. 197-202. https://doi.org/10.1111/j.1365-2583.1996.tb00054.x

AuthorsField, L. M., Crick, S. E. and Devonshire, A. L.
Abstract

The ability of a peach-potato aphid (Myzus persicae) to resist insecticides can depend on both the presence of amplified esterase genes and on differences in their expression. Here we report a polymerase chain reaction-restriction enzyme (PCR-REN) technique which can detect the presence of amplified esterase genes and distinguish between the two possible types of amplified gene (E4 and FE4) and a homologous fragment from susceptible aphids. The technique is quick and sensitive enough to be used on a fraction of an aphid or an individual embryo. Furthermore, it can be combined with a pre-PCR digestion using a methylation-sensitive enzyme (Hpall) to determine whether or not the esterase genes contain 5-methylcytosine, the presence or absence of which is known to correlate with changes in gene expression.

KeywordsBiochemistry & Molecular Biology; Entomology
Year of Publication1996
JournalInsect Molecular Biology
Journal citation5 (3), pp. 197-202
Digital Object Identifier (DOI)https://doi.org/10.1111/j.1365-2583.1996.tb00054.x
PubMed ID8799738
Open accessPublished as non-open access
Funder project or code235
438
ISSN09621075
PublisherWiley

Permalink - https://repository.rothamsted.ac.uk/item/878q4/polymerase-chain-reaction-based-identification-of-insecticide-resistance-genes-and-dna-methylation-in-the-aphid-myzus-persicae-sulzer

20 total views
0 total downloads
0 views this month
0 downloads this month