Development of an acute and chronic ecotoxicity assay using lux -marked Rhizobium leguminosarum biovar trifolii

A - Papers appearing in refereed journals

Paton, G. I., Palmer, G., Burton, M., Rattray, E. A. S., McGrath, S. P., Glover, L. A. and Killham, K. 1997. Development of an acute and chronic ecotoxicity assay using lux -marked Rhizobium leguminosarum biovar trifolii. Letters in Applied Microbiology. 24 (4), pp. 296-300.

AuthorsPaton, G. I., Palmer, G., Burton, M., Rattray, E. A. S., McGrath, S. P., Glover, L. A. and Killham, K.
Abstract

A soil isolate of Rhizobium leguminosarum bv. trifolii was marked with a lux CDABE gene cassette to enable the expression of bioluminescence. The suitability of the bacterium as a soil pollution biosensor was assessed using acute and chronic assays. Bacterial bioluminescence responded sensitively to the metals studied, The order of sensitivity was found to be Cd > Ni = Zn > Cu for the acute test and Cd > Ni = Zn = Cu for the chronic test. The sensitive response of the biosensor highlighted its potential for use as an indicator of soil pollution.

KeywordsBiotechnology & Applied Microbiology; Microbiology
Year of Publication1997
JournalLetters in Applied Microbiology
Journal citation24 (4), pp. 296-300
Digital Object Identifier (DOI)doi:10.1046/j.1472-765X.1997.00071.x
PubMed ID9134778
Open accessPublished as bronze (free) open access
Funder project or code923
219
443
Project: 013280
Publisher's version
PublisherWiley
ISSN0266-8254

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