The role of B-type esterases in conferring insecticide resistance in the tobacco whitefly, Bemisia tabaci (Genn)

A - Papers appearing in refereed journals

Byrne, F. J., Gorman, K. J., Cahill, M., Denholm, I. and Devonshire, A. L. 2000. The role of B-type esterases in conferring insecticide resistance in the tobacco whitefly, Bemisia tabaci (Genn). Pest Management Science. 56 (10), pp. 867-874. https://doi.org/10.1002/1526-4998(200010)56:10<867::AID-PS218>3.0.CO;2-P

AuthorsByrne, F. J., Gorman, K. J., Cahill, M., Denholm, I. and Devonshire, A. L.
Abstract

Separation of non-specific esterases on electrophoretic gels has played a key role in distinguishing between races or biotypes of the tobacco whitefly, Bemisia tabaci. One intensively staining esterase in particular (termed E-0.14) has assumed significance as a diagnostic of B-type whiteflies (aka Bemisia argentifolii), despite any knowledge of its biological function. In this study, a whitefly strain (B-Null) homozygous for a null allele at the E-0.14 locus that had been isolated from a B-type population was used to demonstrate a significant role for E-0.14 in resistance of B-type populations to pyrethroids but not to organophosphates (OPs). Bioassays with pyrethroids, following pretreatment with sub-lethal doses of the OP profenofos (to inhibit esterase activity), coupled with metabolism studies with radiolabelled permethrin, supported the conclusion that pyrethroid resistance in a range of B-type strains expressing E-0.14 was primarily due to increased ester hydrolysis. In the same strains, OP resistance appeared to be predominantly conferred by a modification to the target-site enzyme acetylcholinesterase. (C) 2000 Society of Chemical Industry.

KeywordsAgronomy; Entomology
Year of Publication2000
JournalPest Management Science
Journal citation56 (10), pp. 867-874
Digital Object Identifier (DOI)https://doi.org/10.1002/1526-4998(200010)56:10<867::AID-PS218>3.0.CO;2-P
Open accessPublished as non-open access
Funder project or code438
ISSN1526498X
PublisherWiley

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