High-performance liquid chromatographic separation of natural and synthetic desulphoglucosinolates and their chemical validation by UV, NMR and chemical ionisation-MS methods

A - Papers appearing in refereed journals

Kiddle, G., Bennett, R. N., Botting, N. P., Davidson, N. E., Robertson, A. A. B. and Wallsgrove, R. M. 2001. High-performance liquid chromatographic separation of natural and synthetic desulphoglucosinolates and their chemical validation by UV, NMR and chemical ionisation-MS methods. Phytochemical Analysis. 12 (4), pp. 226-242. https://doi.org/10.1002/pca.589

AuthorsKiddle, G., Bennett, R. N., Botting, N. P., Davidson, N. E., Robertson, A. A. B. and Wallsgrove, R. M.
Abstract

Methods are described for the optimised extraction, desulphation and HPLC separation of desulphoglucosinolates, These methods provide rapid separation, identification and quantitative measurements of glucosinolates extracted from Brassica napus L and related crops, of unusual glucosinolates found in crucifer weed species, and also of synthetic alkylglucosinolates, The desulphoglucosinolates used in these studies were either chemically synthesised (at least one example from each major structural class), or purified from various plant sources. Validation of the identities of the desulphoglucosinolates was by comparison of retention times with standards, and by UV,H-1- and C-13-NMR and chemical ionisation MS analysis. A list of useful species, and the specific tissues, from which high concentrations of standards can be extracted is included. Copyright (C) 2001 John Wiley & Sons, Ltd.

KeywordsBiochemical Research Methods; Plant Sciences; Chemistry, Analytical
Year of Publication2001
JournalPhytochemical Analysis
Journal citation12 (4), pp. 226-242
Digital Object Identifier (DOI)https://doi.org/10.1002/pca.589
PubMed ID11705329
Open accessPublished as non-open access
Funder project or code413
521
Project: 4199
ISSN09580344
PublisherWiley

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