Cloning and sequence analysis of the eburicol 14alpha-demethylase encoding gene (CYP51 ) from the Japanese pear scab fungus Venturia nashicola
A - Papers appearing in refereed journals
Cools, H. J., Ishii, H., Butters, J. A. and Hollomon, D. W. 2002. Cloning and sequence analysis of the eburicol 14alpha-demethylase encoding gene (CYP51 ) from the Japanese pear scab fungus Venturia nashicola. Journal of Phytopathology. 150 (42986), pp. 444-450. https://doi.org/10.1046/j.1439-0434.2002.00776.x
| Authors | Cools, H. J., Ishii, H., Butters, J. A. and Hollomon, D. W. |
|---|---|
| Abstract | The slow growth of many lamentous fungi in vitro prevents rapid isolation of large quantities of genomic DNA, thereby hampering molecular studies. Using a polymerase chain reaction (PCR)-based adapter ligation cloning technique that requires only nanogram quantities of genomic DNA we have successfully cloned the complete CYP51 gene encoding the eburicol 14 a demethylase, the target for DMI (Demethylation-inhibiting) fungicides, from the Japanese pear scab fungus, Venturia nashicola. Analysis of predicted amino acid sequences of CYP51s from strains less sensitive to DMIs revealed no alterations when compared to the sensitive reference strain. |
| Keywords | Plant Sciences |
| Year of Publication | 2002 |
| Journal | Journal of Phytopathology |
| Journal citation | 150 (42986), pp. 444-450 |
| Digital Object Identifier (DOI) | https://doi.org/10.1046/j.1439-0434.2002.00776.x |
| Open access | Published as non-open access |
| Funder project or code | 422 |
| 507 | |
| ISSN | 09311785 |
| Publisher | Wiley |
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Cools, H. J., Bean, T. P., Motteram, J., Gilbert, S. R. and Fraaije, B. A. 2007. Molecular mechanisms associated with altered azole sensitivity in Mycosphaerella graminicola. Proceedings 16th International Plant Protection Congress, Glasgow, 15-18 October 2007 . pp. 406-407
Transcriptome profiling of the response of Mycosphaerella graminicola isolates to an azole fungicide using cDNA microarrays
A - Papers appearing in refereed journals
Cools, H. J., Fraaije, B. A., Bean, T. P., Antoniw, J. F. and Lucas, J. A. 2007. Transcriptome profiling of the response of Mycosphaerella graminicola isolates to an azole fungicide using cDNA microarrays. Molecular Plant Pathology. 8 (5), pp. 639-651. https://doi.org/10.1111/J.1364-3703.2007.00411.X
Molecular mechanisms correlated with reduced azole sensitivity in Mycosphaerella graminicola
C2 - Non-edited contributions to conferences
Cools, H. J. 2007. Molecular mechanisms correlated with reduced azole sensitivity in Mycosphaerella graminicola.
Sterol content analysis of Mycosphaerella graminicola isolates with reduced sensitivity to triazole fungicides
C2 - Non-edited contributions to conferences
Bean, T. P., Cools, H. J., Fraaije, B. A. and Lucas, J. A. 2007. Sterol content analysis of Mycosphaerella graminicola isolates with reduced sensitivity to triazole fungicides.
Comparison of Leptosphaeria biglobosa- induced and chemically induced systemic resistance to L. maculans in Brassica napus
A - Papers appearing in refereed journals
Liu, S-Y., Liu, R-H., Latunde-Dada, A. O., Cools, H. J., Foster, S. J., Huang, Y-J. and Fitt, B. D. L. 2007. Comparison of Leptosphaeria biglobosa- induced and chemically induced systemic resistance to L. maculans in Brassica napus. Chinese Science Bulletin. 52, pp. 1053-1062. https://doi.org/10.1007/s11434-007-0181-5
A novel substitution 1381V in the sterol 14alpha-demethylase (CYP51) of Mycosphaerella graminicola is differentially selected by azole fungicides
A - Papers appearing in refereed journals
Fraaije, B. A., Cools, H. J., Kim, S-H., Motteram, J., Clark, W. S. and Lucas, J. A. 2007. A novel substitution 1381V in the sterol 14alpha-demethylase (CYP51) of Mycosphaerella graminicola is differentially selected by azole fungicides. Molecular Plant Pathology. 8 (3), pp. 245-254. https://doi.org/10.1111/j.1364-3703.2007.00388.X
Methods to detect fungicide resistance: from broth dilution to real-time PCR
C1 - Edited contributions to conferences/learned societies
Fraaije, B. A. and Cools, H. J. 2006. Methods to detect fungicide resistance: from broth dilution to real-time PCR. Fungicide Resistance: Are We Winning the Battle but Losing the War? Aspects of Applied Biology 78 . pp. 11-15
Transcriptome profiling of the response of Mycosphaerella graminicola isolates to triazole fungicides using cDNA microarrays
C2 - Non-edited contributions to conferences
Cools, H. J., Antoniw, J. F. and Fraaije, B. A. 2006. Transcriptome profiling of the response of Mycosphaerella graminicola isolates to triazole fungicides using cDNA microarrays. Abstracts 11th IUPAC International Congress of Pesticide Chemistry, Kobe, 6-11 August 2006 . pp. 140
Transcriptome profiling of the response of Mycosphaerella graminicola isolates to triazole fungicides using cDNA microarrays
C2 - Non-edited contributions to conferences
Cools, H. J., Antoniw, J. F., Bean, T. P. and Fraaije, B. A. 2006. Transcriptome profiling of the response of Mycosphaerella graminicola isolates to triazole fungicides using cDNA microarrays. Abstracts 3rd COGEME Interest Group Meeting, Manchester, 7-8 November 2006 . pp. 28
Evolution of azole resistance mechanisms in UK populations of Mycosphaerella graminicola
C1 - Edited contributions to conferences/learned societies
Cools, H. J. and Fraaije, B. A. 2006. Evolution of azole resistance mechanisms in UK populations of Mycosphaerella graminicola. Fungicide Resistance: Are We Winning the Battle but Losing the War? Aspects of Applied Biology 78 . pp. 21-27
In planta expression analysis in Mycosphaerella graminicola of genes encoding efflux proteins
C2 - Non-edited contributions to conferences
Bean, T. P., Cools, H. J., Fraaije, B. A. and Lucas, J. A. 2006. In planta expression analysis in Mycosphaerella graminicola of genes encoding efflux proteins. Asbtracts APS CPS MSA Joint Meeting on Plant Pathology at the Biological Crossroads, Quebec, 29 July-2 August 2006 . pp. 11
Impact of changes in the target P450 CYP51 enzyme associated with altered triazole-sensitivity in fungal pathogens of cereal crops
A - Papers appearing in refereed journals
Cools, H. J., Fraaije, B. A., Kim, S. H. and Lucas, J. A. 2006. Impact of changes in the target P450 CYP51 enzyme associated with altered triazole-sensitivity in fungal pathogens of cereal crops. Biochemical Society Transactions. 34 (6), pp. 1219-1222. https://doi.org/10.1042/BST0341219
Exploring appressorial formation in the cereal biotroph powdery mildew
C2 - Non-edited contributions to conferences
Gilbert, S. R., Fraaije, B. A., Bailey, A., Cools, H. J. and Lucas, J. A. 2006. Exploring appressorial formation in the cereal biotroph powdery mildew. Abstracts 8th European Conference on Fungal Genetics, Vienna, 8-11 April 2006 . pp. 165
Testing an experimental fungicide for in vitro activity against Mycosphaerella graminicola (anamorph Septoria tritici ) (Confidential report for Cropsolution Inc)
D1 - Technical reports: non-confidential
Lucas, J. A., Bean, T., Cools, H. J. and Fraaije, B. A. 2005. Testing an experimental fungicide for in vitro activity against Mycosphaerella graminicola (anamorph Septoria tritici ) (Confidential report for Cropsolution Inc).
Exploring appressorial formation in the biotrophic cereal fungus powdery mildew
C2 - Non-edited contributions to conferences
Gilbert, S., Cools, H. J., Fraaije, B. A., Bailey, A. and Lucas, J. A. 2005. Exploring appressorial formation in the biotrophic cereal fungus powdery mildew. Abstracts 23rd Fungal Genetics Conference, Asilomar, 15-20 March 2005 . pp. 177
The role of ascospores in further spread of QoI-resistant cytochrome b alleles (G143A) in field populations of Mycosphaerella graminicola
A - Papers appearing in refereed journals
Fraaije, B. A., Cools, H. J., Fountaine, J., Lovell, D. J., Motteram, J., West, J. S. and Lucas, J. A. 2005. The role of ascospores in further spread of QoI-resistant cytochrome b alleles (G143A) in field populations of Mycosphaerella graminicola. Phytopathology. 95 (8), pp. 933-941. https://doi.org/10.1094/PHYTO-95-0933
Molecular examination of Septoria tritici isolates with reduced sensitivities to triazoles
C1 - Edited contributions to conferences/learned societies
Cools, H. J., Fraaije, B. A. and Lucas, J. A. 2005. Molecular examination of Septoria tritici isolates with reduced sensitivities to triazoles. Dehne, H-W., Lyr, H., Russell, P. E., Kuck, K. H. and Gisi, U. (ed.) Modern Fungicides and Antifungal Compounds IV: Proceedings of the 14th International Reinhardsbrunn Symposium, Friedrichroda, 25-29 April 2004 . British Crop Protection Council (BCPC). pp. 103-114
Molecular mechanisms correlated with changes in triazole sensitivity in isolates of Mycosphaerella graminicola
C1 - Edited contributions to conferences/learned societies
Cools, H. J., Fraaije, B. A. and Lucas, J. A. 2005. Molecular mechanisms correlated with changes in triazole sensitivity in isolates of Mycosphaerella graminicola. Proceedings BCPC International Congress - Crop Science and Technology, Glasgow, 31 October-2 November 2005 . pp. 267-274
Using cDNA microarrays to profile the response of Mycosphaerella graminicola to triazole fungicides
C2 - Non-edited contributions to conferences
Cools, H. J., Fraaije, B. A., Keon, J. P. R., Antoniw, J. F. and Lucas, J. A. 2005. Using cDNA microarrays to profile the response of Mycosphaerella graminicola to triazole fungicides. Abstracts COGEME Interest Group Meeting, Manchester, 8-9 November 2005 .
Leptosphaeria biglobosa can induce higher resistance of Brassica napus to Phoma stem canker by triggering earlier JA/ET pathway and stronger SA pathway both locally and systematically
C2 - Non-edited contributions to conferences
Liu, R., Liu, S., Fitt, B. D. L., Cools, H. J., Latunde-Dada, A. O., Huang, Y-J., Li, Z. and Lucas, J. A. 2004. Leptosphaeria biglobosa can induce higher resistance of Brassica napus to Phoma stem canker by triggering earlier JA/ET pathway and stronger SA pathway both locally and systematically. Abstracts Discovery, Development and Delivery in Plant Pathology: 7th Conference of the European Foundation for Plant Pathology and BSPP Presidential Meeting 2004, Aberdeen, 5-10 September 2004 . pp. 38
Molecular mechanisms conferring reduced sensitivities to triazoles in UK isolates of Septoria tritici
C2 - Non-edited contributions to conferences
Cools, H. J., Fraaije, B. A. and Lucas, J. A. 2004. Molecular mechanisms conferring reduced sensitivities to triazoles in UK isolates of Septoria tritici.
Menadione sodium bisulphite: a novel plant defence activator which enhances local and systemic resistance to infection by Leptosphaeria maculans in oilseed rape
A - Papers appearing in refereed journals
Borges, A. A., Cools, H. J. and Lucas, J. A. 2003. Menadione sodium bisulphite: a novel plant defence activator which enhances local and systemic resistance to infection by Leptosphaeria maculans in oilseed rape. Plant Pathology. 52 (4), pp. 429-436. https://doi.org/10.1046/j.1365-3059.2003.00877.x
Pre-treatment of cucumber plants with acibenzolar-S-methyl systemically primes a phenylalanine ammonia lyase gene (PAL1 ) for enhanced expression upon attack with a pathogenic fungus
A - Papers appearing in refereed journals
Cools, H. J. and Ishii, H. 2002. Pre-treatment of cucumber plants with acibenzolar-S-methyl systemically primes a phenylalanine ammonia lyase gene (PAL1 ) for enhanced expression upon attack with a pathogenic fungus. Physiological and Molecular Plant Pathology. 61, pp. 273-280.
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