Cloning and sequence analysis of the eburicol 14alpha-demethylase encoding gene (CYP51 ) from the Japanese pear scab fungus Venturia nashicola

A - Papers appearing in refereed journals

Cools, H. J., Ishii, H., Butters, J. A. and Hollomon, D. W. 2002. Cloning and sequence analysis of the eburicol 14alpha-demethylase encoding gene (CYP51 ) from the Japanese pear scab fungus Venturia nashicola. Journal of Phytopathology. 150 (42986), pp. 444-450.

AuthorsCools, H. J., Ishii, H., Butters, J. A. and Hollomon, D. W.
Abstract

The slow growth of many lamentous fungi in vitro prevents rapid isolation of large quantities of genomic DNA, thereby hampering molecular studies. Using a polymerase chain reaction (PCR)-based adapter ligation cloning technique that requires only nanogram quantities of genomic DNA we have successfully cloned the complete CYP51 gene encoding the eburicol 14 a demethylase, the target for DMI (Demethylation-inhibiting) fungicides, from the Japanese pear scab fungus, Venturia nashicola. Analysis of predicted amino acid sequences of CYP51s from strains less sensitive to DMIs revealed no alterations when compared to the sensitive reference strain.

KeywordsPlant Sciences
Year of Publication2002
JournalJournal of Phytopathology
Journal citation150 (42986), pp. 444-450
Digital Object Identifier (DOI)doi:10.1046/j.1439-0434.2002.00776.x
Open accessPublished as non-open access
Funder project or code422
507
ISSN09311785
PublisherWiley

Permalink - https://repository.rothamsted.ac.uk/item/88wqx/cloning-and-sequence-analysis-of-the-eburicol-14alpha-demethylase-encoding-gene-cyp51-from-the-japanese-pear-scab-fungus-venturia-nashicola

3 total views
0 total downloads
1 views this month
0 downloads this month