Genetic manipulation of rubisco: Chromatium vinosum rbc L is expressed in Nicotiana tabacum but does not form a functional protein

Madgwick, P. J., Colliver, S. P., Banks, F. M., Habash, D. Z., Dulieu, H., Parry, Martin and Paul, Matthew (2002) Genetic manipulation of rubisco: Chromatium vinosum rbc L is expressed in Nicotiana tabacum but does not form a functional protein. Annals of Applied Biology, 140 (1). pp. 13-19. 10.1111/j.1744-7348.2002.tb00152.x
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N. tabacum lines that lacked functional Rubisco were transformed with plasmids encoding a chloroplast transit peptide in frame with C. vinosum rbcL and stable transformants generated. However, the transgene was transcribed at a low level and no Rubisco activity or C. vinosum large subunits were detectable in any line.

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