A - Papers appearing in refereed journals
Huang, Y-J., Evans, N., Li, Z-Q., Eckert, M., Chevre, A-M, Renard, M. and Fitt, B. D. L. 2006. Temperature and leaf wetness duration affect phenotypic expression of Rlm6 -mediated resistance to Leptosphaeria maculans in Brassica napus. New Phytologist. 170 (1), pp. 129-141.
|Authors||Huang, Y-J., Evans, N., Li, Z-Q., Eckert, M., Chevre, A-M, Renard, M. and Fitt, B. D. L.|
Near-isogenic Brassica napus lines carrying/lacking resistance gene Rlm6 were used to investigate the effects of temperature and leaf wetness duration on phenotypic expression of Rlm6-mediated resistance. Leaves were inoculated with ascospores or conidia of Leptosphaeria maculans carrying the effector gene AvrLm6. Incubation period to the onset of lesion development, number of lesions and lesion diameter were assessed. Symptomless growth of L. maculans from leaf lesions to stems was investigated using a green fluorescent protein (GFP) expressing isolate carrying AvrLm6. L. maculans produced large grey lesions on Darmor (lacking Rlm6) at 5-25 degrees C and DarmorMX (carrying Rlm6) at 25 degrees C, but small dark spots and 'green islands' on DarmorMX at 5-20 degrees C. With increasing temperature/wetness duration, numbers of lesions/spots generally increased. GFP-expressing L. maculans grew from leaf lesions down leaf petioles to stems on DarmorMX at 25 degrees C but not at 15 degrees C. We conclude that temperature and leaf wetness duration affect the phenotypic expression of Rlm6-mediated resistance in leaves and subsequent L. maculans spread down petioles to produce stem cankers.
|Year of Publication||2006|
|Journal citation||170 (1), pp. 129-141|
|Digital Object Identifier (DOI)||doi:10.1111/j.1469-8137.2006.01651.x|
|Open access||Published as non-open access|
|Funder project or code||507|
|SECURE - Stem canker of oilseed rape: molecular tools and mathematical modelling to deploy resistance|
|Improving strategies to manage oilseed rape resistance to stem canker in East Anglia|
|Oilseed Rape Genetic Improvement Network (OREGIN)|
Permalink - https://repository.rothamsted.ac.uk/item/898zx/temperature-and-leaf-wetness-duration-affect-phenotypic-expression-of-rlm6-mediated-resistance-to-leptosphaeria-maculans-in-brassica-napus