The alternative pathway C20 delta8-desaturase from the non-photosynthetic organism Acanthamoeba castellanii is an atypical cytochrome b5 -fusion desaturase

A - Papers appearing in refereed journals

Sayanova, O. V., Haslam, R. P., Qi, B., Lazarus, C. M. and Napier, J. A. 2006. The alternative pathway C20 delta8-desaturase from the non-photosynthetic organism Acanthamoeba castellanii is an atypical cytochrome b5 -fusion desaturase. FEBS Letters. 580 (8), pp. 1946-1952.

AuthorsSayanova, O. V., Haslam, R. P., Qi, B., Lazarus, C. M. and Napier, J. A.
Abstract

A cDNA encoding a C-20 Delta 8-desaturase was isolated from the free-living soil amoeba, A canthamoeba castellanii and functionally characterised by heterologous expression. The open reading frame of the A. castellanii CM A8-desaturase showed similarity to other microsomal front-end desaturases, but the N-terminal domain contained a variant form of the conserved heme-binding motif in which H-P-G-G is replaced by H-P-A-G. Co-expression of the A. castellani Delta 8-desaturase with the Isochrysis galbana Delta 9-elongase in transgenic Arabidopsis plants confirmed the activity observed in yeast and its role in the alternative pathway for C-20 polyunsaturated fatty acid synthesis. Acyl-CoA profiles of these transgenic plants revealed an unexpected accumulation of C-20 fatty acids in the acyl-CoA pool. This is the first report of an alternative pathway C-20 Delta 8-desaturase from a non-photosynthetic organism, and also the first report of a front-end desaturase lacking the canonical cytochrome b(5) domain. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

KeywordsBiochemistry & Molecular Biology; Biophysics; Cell Biology
Year of Publication2006
JournalFEBS Letters
Journal citation580 (8), pp. 1946-1952
Digital Object Identifier (DOI)doi:10.1016/j.febslet.2006.02.062
PubMed ID16530193
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or code503
ISSN00145793
PublisherWiley
Grant IDBBS/E/C/00004156

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