The use of monoclonal phage antibodies for the detection of the pinewood nematode Bursaphelenchus xylophilus

C2 - Non-edited contributions to conferences

Fonseca, L., Curtis, R. H. C., Halsey, K., Vieira Dos Santos, M. C. and Abrantes, I. M. De O. 2006. The use of monoclonal phage antibodies for the detection of the pinewood nematode Bursaphelenchus xylophilus. Abstracts 28th International Symposium of the European Society of Nematologists, Blagoevgrad, Bulgaria, 5-9 June 2006 . Wiley. doi:10.1111/j.1744-7348.1991.tb04868.x

AuthorsFonseca, L., Curtis, R. H. C., Halsey, K., Vieira Dos Santos, M. C. and Abrantes, I. M. De O.
TypeC2 - Non-edited contributions to conferences
Abstract

Information on infectivity of the aphids which invade sugar beet root crops each Spring is required for forecasting incidence and providing advice on control of virus ye lows. Monoclonal antibodies, produced in the USA to barley yellow dwarf virus (BYDV) and in Canada to beet western yellows virus (BWYV), were used to distinguish between sugar-beet-infecting strains of the luteovirus beet mild yellowing virus (BMYV), and the non-beet-infecting strains of the closely-related BWYV in plant and aphid tissue. Totals of 773 immigrant winged Myzus persicae and 124 Macrosiphum euphorbiae were caught in water traps in a crop of sugar beet between 25 April and 5 August 1990. Using the monoclonal antibodies and an amplified ELISA, 67% M. persicae and 19% M. euphorbiae were shown to contain BWYV; 8% M. persicae and 7% M. euphorbiae contained BMYV. In studies with live winged aphids collected from the same sugar beet field during May, 25 of 60 M. persicae and two of 13 M. euphorbiae transmitted BWYV to the indicator host plant Montia perfoliata; two M. persicae and two M. euphorbiae transmitted BMYV. In another study three of 65 M. persicae and one of three M. euphorbiae in which only BWYV was detected, transmitted this virus to sugar beet.

KeywordsAgriculture, Multidisciplinary
Year of Publication2006
Digital Object Identifier (DOI)doi:10.1111/j.1744-7348.1991.tb04868.x
Journal citation(2), p. 152
PublisherWiley
Funder project or code506
Molecular genetics of interactions between plants and sedentary nematodes
ISSN00034746

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