Synthesis of low molecular weight thiols in response to Cd exposure in Thlaspi caerulescens

A - Papers appearing in refereed journals

Hernandez-Allica, J., Garbisu, C., Becerril, J. M., Barrutia, O., Garcia-Plazaola, J. I., Zhao, F-J. and McGrath, S. P. 2006. Synthesis of low molecular weight thiols in response to Cd exposure in Thlaspi caerulescens. Plant, Cell & Environment. 29 (7), pp. 1422-1429.

AuthorsHernandez-Allica, J., Garbisu, C., Becerril, J. M., Barrutia, O., Garcia-Plazaola, J. I., Zhao, F-J. and McGrath, S. P.
Abstract

In this study, we investigated the accumulation of phytochelatins (PCs) and other low molecular weight (LMW) thiols in response to Cd exposure in two contrasting ecotypes differing in Cd accumulation. Using a root elongation test, we found that the highly accumulating ecotype Ganges was more tolerant to Cd than the low Cd-accumulation ecotype Prayon.L-buthionine-(S,R)-sulphoximine (BSO), a potent inhibitor of the gamma-glutamylcysteine synthetase (gamma-ECS) (an enzyme involved in the PC biosynthetic pathway), increased the Cd sensitivity of Prayon, but had no effect on Ganges. Although PC accumulation increased in response to Cd exposure, no significant differences were observed between the two ecotypes. Cd exposure induced a dose-dependent accumulation of both Cys and a still unidentified LMW thiol in roots of both ecotypes. Root accumulation of Cys and this thiol was higher in Ganges than in Prayon; the ecotypic differences were more pronounced when the plants were treated with BSO. These findings suggest that PCs do not contribute to the Cd hypertolerance displayed by the Ganges ecotype of Thlaspi caerulescens, whereas Cys and other LMW thiols might be involved.

KeywordsPlant Sciences
Year of Publication2006
JournalPlant, Cell & Environment
Journal citation29 (7), pp. 1422-1429
Digital Object Identifier (DOI)doi:10.1111/j.1365-3040.2006.01525.x
PubMed ID17080963
Open accessPublished as non-open access
Funder project or code512
ISSN01407791
PublisherWiley

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