Analysis of the role of COMATOSE and peroxisomal beta-oxidation in the determination of germination potential in Arabidopsis

A - Papers appearing in refereed journals

Footitt, S., Marquez, J., Schmuths, H., Baker, A., Theodoulou, F. L. and Holdsworth, M. 2006. Analysis of the role of COMATOSE and peroxisomal beta-oxidation in the determination of germination potential in Arabidopsis. Journal of Experimental Botany. 57 (11), pp. 2805-2814.

AuthorsFootitt, S., Marquez, J., Schmuths, H., Baker, A., Theodoulou, F. L. and Holdsworth, M.
Abstract

Comparative physiological analysis of mutant Arabidopsis seeds under defined environmental conditions was used to analyse the relative contributions of components of peroxisomal beta-oxidation in the control of seed germination potential. The COMATOSE (CTS) and KAT2 loci were shown to play essential roles in regulating germination and establishment potentials, whereas LACS6 and LACS7 loci only influenced establishment following germination. The viability and desiccation tolerance of three different mutant alleles of CTS were shown to be intermediate between that of dormant and non-dormant wild-type seeds. Analysis of ttg-1 cts-1 double mutant seeds demonstrated that the cts lesion did not influence after-ripening capacity. These data demonstrate the importance of peroxisomal beta-oxidation in the control of germination potential, but suggest that breakdown of stored lipid is not an important prerequisite for germination. A function is suggested for CTS following after-ripening within pathways related to the progression of germination prior to radicle emergence.

KeywordsArabidopsis; beta-oxidation; COMATOSE; gene expression; germination; peroxisome; physiological genetics
Year of Publication2006
JournalJournal of Experimental Botany
Journal citation57 (11), pp. 2805-2814
Digital Object Identifier (DOI)doi:10.1093/jxb/erl045
Open accessPublished as ‘gold’ (paid) open access
Funder project or code503
Cloning and characterisation of genes involved in xenobiotic metabolism
Functional genomics of shoot meristem dormancy
Publisher's version
Copyright license
CC BY-NC
File Access Level
Open
Output statusPublished
Publication dates
Online14 Jul 2006
Publication process dates
Accepted29 Apr 2006
ISSN0022-0957
PublisherOxford University Press (OUP)

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