Movement of transgenic plant-expressed Bt Cry1Ac proteins through high trophic levels

A - Papers appearing in refereed journals

Wei, W., Schuler, T. H., Clark, S. J., Stewart, C. N. Jr and Poppy, G. M. 2008. Movement of transgenic plant-expressed Bt Cry1Ac proteins through high trophic levels. Journal of Applied Entomology. 132 (1), pp. 1-11.

AuthorsWei, W., Schuler, T. H., Clark, S. J., Stewart, C. N. Jr and Poppy, G. M.
Abstract

The movement of Bacillus thuringiensis (Berliner) (Bt) Cry1Ac endotoxin through high trophic levels was assessed to help elucidate the effects of Bt toxin on non-target insects. The diamondback moth (Plutella xylostella L., Lepidoptera: Plutellidae), the parasitic wasp (Cotesia vestalis Haliday, Hymenoptera: Braconidae) and the predatory green lacewing Chrysoperla carnea (Stephen) (Neuroptera: Chrysopidae) were used as a model system in this laboratory study. Bt-resistant P. xylostella larvae fed Cry1Ac-expressing transgenic oilseed rape (OSR, Brassica napus L., Cruciferae), before and after parasitization by C. vestalis, consumed Cry1Ac with the ingested plant material but only a proportion of Cry1Ac consumed was recovered from the bodies and faeces of P. xylostella larvae. Cry1Ac was not detected in newly emerged parasitoid larvae. In contrast, Cry1Ac was detected in C. carnea larvae fed on resistant P. xylostella larvae reared on Bt OSR. However, no Cry1Ac could be detected in C. carnea larvae when the lacewings were transferred to P. xylostella larvae reared on conventional OSR and tested 24-48 h. The metabolizing ability of Cry1Ac is discussed for the larvae of P. xylostella and C. carnea.

KeywordsEntomology
Year of Publication2008
JournalJournal of Applied Entomology
Journal citation132 (1), pp. 1-11
Digital Object Identifier (DOI)doi:10.1111/j.1439-0418.2007.01242.x
Open accessPublished as non-open access
Funder project or codeSEF
Centre for Mathematical and Computational Biology (MCB)
ISSN09312048
PublisherWiley

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