Serological relationships among the over-expressed coat proteins of allexiviruses

Lu, Y-W., Chen, J., Zheng, H-Y., Adams, Michael and Chen, J-P. (2008) Serological relationships among the over-expressed coat proteins of allexiviruses. Journal of Phytopathology, 156 (4). pp. 251-255. 10.1111/j.1439-0434.2007.01323.x
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Coat protein genes of six members of the genus Allexivirus, Garlic viruses -A, -B, -C, -D, -E and -X, were over-expressed in bacteria and used to prepare polyclonal antibodies. In western blot analysis, Garlic virus D (GarV-D) and GarV-E were closely related serologically and there were weaker relationships between some other pairs, particularly GarV-E with GarV-A, GarV-X with GarV-A, GarV-X with GarV-B and GarV-X with GarV-C. Sequence analysis suggested that there was no simple relationship between amino acid identity and serological properties.

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