Cloning and characterisation of a phenyltransferase from the aphid Myzus persicae with potential involvement in alarm pheromone synthesis

A - Papers appearing in refereed journals

Lewis, M. J., Prosser, I. M., Mohib, A. and Field, L. M. 2008. Cloning and characterisation of a phenyltransferase from the aphid Myzus persicae with potential involvement in alarm pheromone synthesis. Insect Molecular Biology. 17 (4), pp. 437-443.

AuthorsLewis, M. J., Prosser, I. M., Mohib, A. and Field, L. M.
Abstract

The majority of aphid species release an alarm pheromone with the most common component being the sesquiterpene (E)-beta-farnesene, sometimes accompanied by other sesquiterpenes or monoterpenes. The genes/enzymes involved in the production of these compounds have not been identified in aphids although some components of isoprenoid biosynthesis have been identified in other insect species. Here we report the cloning, expression and characterisation of a prenyltransferase from the aphid Myzus persicae which can act as a farnesyl pyrophosphate synthase or a geranyl pyrophosphate synthase to produce both sesquiterpenes and monoterpenes and hence could be responsible for the biosynthesis of the observed components of the alarm pheromones. In addition, the enzyme can utilise geranyl pyrophosphate to produce farnesyl pyrophosphate showing that the synthesis of the latter involves the sequential condensation of isoprenyl pyrophosphate units.

KeywordsBiochemistry & Molecular Biology; Entomology
Year of Publication2008
JournalInsect Molecular Biology
Journal citation17 (4), pp. 437-443
Digital Object Identifier (DOI)doi:10.1111/j.1365-2583.2008.00815.x
PubMed ID18651925
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeCentre for Sustainable Pest and Disease Management (PDM)
Centre for Crop Genetic Improvement (CGI)
Project: 4947
Project: 4848
PublisherWiley
ISSN0962-1075

Permalink - https://repository.rothamsted.ac.uk/item/8q01q/cloning-and-characterisation-of-a-phenyltransferase-from-the-aphid-myzus-persicae-with-potential-involvement-in-alarm-pheromone-synthesis

Restricted files

Publisher's version

Under embargo indefinitely

8 total views
0 total downloads
0 views this month
0 downloads this month