Microhabitat scale methods in microbial ecology

Dennis, P., Miller, A. J., Kilburn, M., Taylor, R., Valsami-Jones, E., Grovenor, C. and Hirsch, P. R. 2009. Microhabitat scale methods in microbial ecology. Abstracts Micro Soil: Integrating Biological, Physical and Chemical Techniques for the Study of Soil Micro-Habitats, Dundee, 16-17 September 2009 . pp. 19

Related outputs

Countering elevated CO2 induced Fe and Zn reduction in Arabidopsis seeds

A - Papers appearing in refereed journals

Sun, P., Isner, J., Coupel-Ledru, A., Zhang, Q., Pridgeon, A., He, Y., Menguer, P., Miller, A. J., Sanders, D., McGrath, S. P., Noothong, F., Liang, Y. and Hetherington, A. 2022. Countering elevated CO2 induced Fe and Zn reduction in Arabidopsis seeds. New Phytologist. 235 (5), pp. 1796-1806. https://doi.org/10.1111/nph.18290

Land management legacy affects abundance and function of the acdS gene in wheat root associated pseudomonads

A - Papers appearing in refereed journals

Ruscoe, H., Taketani, R., Clark, I. M., Lund, G., Hughes, D. J., Dodd, I., Hirsch, P. R. and Mauchline, T. H. 2021. Land management legacy affects abundance and function of the acdS gene in wheat root associated pseudomonads. Frontiers in Microbiology. 12, p. 611339. https://doi.org/10.3389/fmicb.2021.611339

Theory of microbial coexistence in promoting soil–plant ecosystem health

A - Papers appearing in refereed journals

Zhang, N., Nunan, N., Hirsch, P. R., Sun, B., Zhou, J. and Liang, Y. 2021. Theory of microbial coexistence in promoting soil–plant ecosystem health. Biology And Fertility Of Soils. 57, p. 897–911. https://doi.org/10.1007/s00374-021-01586-w

Metagenomic approaches reveal differences in genetic diversity and relative abundance of nitrifying bacteria and archaea in contrasting soils

A - Papers appearing in refereed journals

Clark, I. M., Hughes, D. J., Fu, Q., Abadie, M. and Hirsch, P. R. 2021. Metagenomic approaches reveal differences in genetic diversity and relative abundance of nitrifying bacteria and archaea in contrasting soils . Scientific Reports. 11, p. 15905. https://doi.org/10.1038/s41598-021-95100-9

Microbiome Aggregated Traits and Assembly are More Sensitive to Soil Management than Diversity

A - Papers appearing in refereed journals

Neal, A. L., Hughes, D. J., Clark, I. M., Jansson, J. K. and Hirsch, P. R. 2021. Microbiome Aggregated Traits and Assembly are More Sensitive to Soil Management than Diversity. mSystems. 6 (3), pp. e01056-20. https://doi.org/10.1128/mSystems.01056-20

Century long fertilization reduces stochasticity controlling grassland microbial community succession

A - Papers appearing in refereed journals

Liang, Y., Ning, D., Lu, Z., Zhang, N., Hale, L., Wu, L., Clark, I. M., McGrath, S. P., Storkey, J., Hirsch, P. R., Sun ,B. and Zhou, J. 2020. Century long fertilization reduces stochasticity controlling grassland microbial community succession. Soil Biology and Biochemistry. 151 (Dec), p. 108023. https://doi.org/10.1016/j.soilbio.2020.108023

Sustainable Cropping Requires Adaptation to a Heterogeneous Rhizosphere

A - Papers appearing in refereed journals

Wang, X., Whalley, W. R., Miller, A. J., White, P. J., Zhang, F. and Shen, J. 2020. Sustainable Cropping Requires Adaptation to a Heterogeneous Rhizosphere. Trends in Plant Science. 25 (12), pp. 1194-1202. https://doi.org/10.1016/j.tplants.2020.07.006

Edaphic factors and plants influence denitrification in soils from a long-term arable experiment

A - Papers appearing in refereed journals

Clark, I. M., Fu, Q., Abadie, M., Dixon, E. R., Blaud, A. and Hirsch, P. R. 2020. Edaphic factors and plants influence denitrification in soils from a long-term arable experiment. Scientific Reports. 10 (Article), p. 16053. https://doi.org/10.1038/s41598-020-72679-z

Bacterial and archaeal taxa are reliable indicators of soil restoration across distributed calcareous grasslands

A - Papers appearing in refereed journals

Armbruster, M., Goodall, T., Hirsch, P. R., Ostle, N., Puissant, J., Fagan, K. C., Pywell, R. F. and Griffiths, R. I. 2020. Bacterial and archaeal taxa are reliable indicators of soil restoration across distributed calcareous grasslands . European Journal of Soil Science. https://doi.org/10.1111/ejss.12977

Wheat dwarfing influences selection of the rhizosphere microbiome

A - Papers appearing in refereed journals

Kavamura, V. N., Robinson, R. J., Hughes, D., Clark, I. M., Rossmann, M., De Melo, I.S., Hirsch, P. R., Mendes, R. and Mauchline, T. H. 2020. Wheat dwarfing influences selection of the rhizosphere microbiome. Scientific Reports. 10, p. 1452. https://doi.org/10.1038/s41598-020-58402-y

Development of a defined compost system for the study of plant-microbe interactions

A - Papers appearing in refereed journals

Masters-Clark, E., Hirsch, P. R., Clark, I. M., Paradelo, M. and Mauchline, T. H. 2020. Development of a defined compost system for the study of plant-microbe interactions. Scientific Reports. 10, p. 7521. https://doi.org/10.1038/s41598-020-64249-0

The pH optimum of soil exoenzymes adapt to long term changes in soil pH

A - Papers appearing in refereed journals

Puissant, J., Briony, J., Goodall, T., Mang, D., Blaud, A., Gweon, H. S., Malik, A., Jones, D. L., Clark, I. M., Hirsch, P. R. and Griffiths, R. 2019. The pH optimum of soil exoenzymes adapt to long term changes in soil pH. Soil Biology and Biochemistry. 138, p. 107601. https://doi.org/10.1016/j.soilbio.2019.107601

Effects of urease and nitrification inhibitors on soil N, nitrifier abundance and activity in a sandy loam soil

A - Papers appearing in refereed journals

Fu, Q., Abadie, M., Blaud, A., Carswell, A. M., Misselbrook, T. H., Clark, I. M. and Hirsch, P. R. 2019. Effects of urease and nitrification inhibitors on soil N, nitrifier abundance and activity in a sandy loam soil . Biology And Fertility Of Soils. pp. 1-10. https://doi.org/10.1007/s00374-019-01411-5

Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat

A - Papers appearing in refereed journals

Kavamura, V. N., Robinson, R. J., Hayat, R., Clark, I. M., Hughes, D. J., Rossmann, M., Hirsch, P. R., Mendes, R. and Mauchline, T. H. 2019. Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat. Frontiers in Microbiology. 10 (2625). https://doi.org/10.3389/fmicb.2019.02625

Plant–microbe networks in soil are weakened by century‐long use of inorganic fertilizers

A - Papers appearing in refereed journals

Huang, R., McGrath, S. P., Hirsch, P. R., Clark, I. M., Storkey, J., Wu, L., Zhou, J. and Liang, Y. 2019. Plant–microbe networks in soil are weakened by century‐long use of inorganic fertilizers. Microbial Biotechnology. 12 (6), pp. 1464-1475. https://doi.org/10.1111/1751-7915.13487

Microorganisms Cycling Soil Nutrients

B - Book chapters etc edited externally

Hirsch, P. R. 2019. Microorganisms Cycling Soil Nutrients. in: Modern Soil Microbiology Florida CRC Press. pp. 179-192

The role of plant species and soil condition in the structural development of the rhizosphere

A - Papers appearing in refereed journals

Helliwell, J. R., Sturrock, C. J., Miller, A. J., Whalley, W. R. and Mooney, S. J. 2019. The role of plant species and soil condition in the structural development of the rhizosphere. Plant, Cell and Environment. 42 (6), pp. 1974-1986. https://doi.org/10.1111/pce.13529

The short-term effects of nitrification inhibitors on the abundance and expression of ammonia and nitrite oxidizers in a long-term field experiment comparing land management

A - Papers appearing in refereed journals

Fu, Q., Clark, I. M., Zhu, J., Hu, H. Q. and Hirsch, P. R. 2018. The short-term effects of nitrification inhibitors on the abundance and expression of ammonia and nitrite oxidizers in a long-term field experiment comparing land management. Biology And Fertility Of Soils. 54 (1), pp. 163-172. https://doi.org/10.1007/s00374-017-1249-2

Old meets new: most probable number validation of metagenomic and metatranscriptomic datasets in soil

A - Papers appearing in refereed journals

Mauchline, T. H., Hayat, R., Clark, I. M. and Hirsch, P. R. 2018. Old meets new: most probable number validation of metagenomic and metatranscriptomic datasets in soil. Letters in Applied Microbiology. 66 (1), pp. 14-18. https://doi.org/10.1111/lam.12821

Soil microorganisms: role in soil health

B - Book chapters etc edited externally

Hirsch, P. R. 2018. Soil microorganisms: role in soil health. in: Reicosky, D. (ed.) Managing Soil Health for Sustainable Agriculture. Volume 1: Fundamentals Cambridge, UK Burleigh Dodds. pp. 169-196

Inorganic Nitrogen Application Affects Both Taxonomical and Predicted Functional Structure of Wheat Rhizosphere Bacterial Communities

A - Papers appearing in refereed journals

Nessner-Kavamura-Noguchi, V., Hayat, R., Clark, I. M., Rossmann, M., Mendes, R., Hirsch, P. R. and Mauchline, T. H. 2018. Inorganic Nitrogen Application Affects Both Taxonomical and Predicted Functional Structure of Wheat Rhizosphere Bacterial Communities. Frontiers in Microbiology. 9, p. 1074. https://doi.org/10.3389/fmicb.2018.01074

Phylogenetic distribution, biogeography and the effects of land management upon bacterial non-specific Acid phosphatase Gene diversity and abundance

A - Papers appearing in refereed journals

Neal, A. L., Blackwell, M. S. A., Akkari, E., Clark, I. M., Hirsch, P. R. and Guyomar, C. 2018. Phylogenetic distribution, biogeography and the effects of land management upon bacterial non-specific Acid phosphatase Gene diversity and abundance. Plant and Soil. 427, pp. 175-189. https://doi.org/10.1007/s11104-017-3301-2

The Nodulin 26-like intrinsic membrane protein OsNIP3;2 is involved in arsenite uptake by lateral roots in rice

A - Papers appearing in refereed journals

Chen, Y., Sun, S-K., Tang, Z., Liu, G., Moore, K. L., Maathuis, F. J. M., Miller, A. J., McGrath, S. P. and Zhao, F-J. 2017. The Nodulin 26-like intrinsic membrane protein OsNIP3;2 is involved in arsenite uptake by lateral roots in rice. Journal of Experimental Botany. 68 (11), pp. 3007-3016. https://doi.org/10.1093/jxb/erx165

Is there sufficient Ensifer and Rhizobium species diversity in UK farmland soils to support red clover (Trifolium pratense), white clover (T. repens), lucerne (Medicago sativa) and black medic (M. lupulina)?

A - Papers appearing in refereed journals

Roberts, R., Jackson, R. W., Mauchline, T. H., Hirsch, P. R., Shaw, L. J., Doring, T. F. and Jones, H. E. 2017. Is there sufficient Ensifer and Rhizobium species diversity in UK farmland soils to support red clover (Trifolium pratense), white clover (T. repens), lucerne (Medicago sativa) and black medic (M. lupulina)? Applied Soil Ecology. 120, pp. 35-43. https://doi.org/10.1016/j.apsoil.2017.06.030

Exploitation of endophytes for sustainable agricultural intensification Exploitation of endophytes

A - Papers appearing in refereed journals

Le Cocq, K., Gurr, S. J., Hirsch, P. R. and Mauchline, T. H. 2017. Exploitation of endophytes for sustainable agricultural intensification Exploitation of endophytes. Molecular Plant Pathology. 18 (3), pp. 469-473. https://doi.org/10.1111/mpp.12483

Soil resilience and recovery: rapid community responses to management changes

A - Papers appearing in refereed journals

Hirsch, P. R., Jhurreea, D., Williams, J. K., Murray, P. J., Scott, T., Misselbrook, T. H., Goulding, K. W. T. and Clark, I. M. 2017. Soil resilience and recovery: rapid community responses to management changes. Plant and Soil. 412 (1-2), pp. 283-297. https://doi.org/10.1007/s11104-016-3068-x

Land-use influences phosphatase gene microdiversity in soils

A - Papers appearing in refereed journals

Neal, A. L., Rossmann, M., Brearley, C., Akkari, E., Guyomar, C., Clark, I. M., Allen, E. and Hirsch, P. R. 2017. Land-use influences phosphatase gene microdiversity in soils. Environmental Microbiology. 19 (7), p. 2740–2753. https://doi.org/10.1111/1462-2920.13778

Long-Term Impact of Field Applications of Sewage Sludge on Soil Antibiotic Resistome

A - Papers appearing in refereed journals

Xie, W-Y., McGrath, S. P., Su, J. Q., Hirsch, P. R., Clark, I. M., Shen, Q., Zhu, Y-G. and Zhao, F-J. 2016. Long-Term Impact of Field Applications of Sewage Sludge on Soil Antibiotic Resistome. Environmental Science & Technology. 50 (23), pp. 12602-12611. https://doi.org/10.1021/acs.est.6b02138

OsCHX14 is Involved in the K+ Homeostasis in Rice (Oryza sativa) Flowers

A - Papers appearing in refereed journals

Chen, Y., Ma, J. K., Miller, A. J., Luo, B. B., Wang, M., Zhu, Z. and Ouwerkerk, P. B. F. 2016. OsCHX14 is Involved in the K+ Homeostasis in Rice (Oryza sativa) Flowers. Plant and cell physiology. 57 (7), pp. 1530-1543. https://doi.org/10.1093/pcp/pcw088

Responses of microbial community from tropical pristine coastal soil to crude oil contamination

A - Papers appearing in refereed journals

Morais, D., Pylro, V., Clark, I. M., Hirsch, P. R. and Totola, M. R. 2016. Responses of microbial community from tropical pristine coastal soil to crude oil contamination. PeerJ. 4. https://doi.org/10.7717/peerj.1733

Misguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases

A - Papers appearing in refereed journals

Pylro, V. S., Morais, D. K., Kalks, K. H. M., Roesch, L. F. W., Hirsch, P. R., Totola, M. R. and Yotoko, K. 2016. Misguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases. Journal of Microbiological Methods. 126, pp. 18-23. https://doi.org/10.1016/j.mimet.2016.04.012

Endophytic bacterial community composition in wheat (Triticum aestivum) is determined by plant tissue type, developmental stage and soil nutrient availability

A - Papers appearing in refereed journals

Robinson, R. J., Fraaije, B. A., Clark, I. M., Jackson, R. W., Hirsch, P. R. and Mauchline, T. H. 2016. Endophytic bacterial community composition in wheat (Triticum aestivum) is determined by plant tissue type, developmental stage and soil nutrient availability. Plant and Soil. 405 (1-2), pp. 381-396. https://doi.org/10.1007/s11104-015-2495-4

Pseudomonas spp. diversity is negatively associated with suppression of the wheat take-all pathogen

A - Papers appearing in refereed journals

Mehrabi, Z., Mcmillan, V. E., Clark, I. M., Canning, G., Hammond-Kosack, K. E., Preston, G., Hirsch, P. R. and Mauchline, T. H. 2016. Pseudomonas spp. diversity is negatively associated with suppression of the wheat take-all pathogen. Scientific Reports. 6. https://doi.org/10.1038/srep29905

The unique contribution of Rothamsted to ecological research at large temporal scales

B - Book chapters etc edited externally

Storkey, J., Macdonald, A. J., Bell, J. R., Clark, I. M., Gregory, A. S., Hawkins, N. J., Hirsch, P. R., Todman, L. C. and Whitmore, A. P. 2016. The unique contribution of Rothamsted to ecological research at large temporal scales. in: Dumbrell, A. J., Kordas, R. L. and Woodward, G. (ed.) Advances in Ecological Research 55: Large-Scale Ecology: Model Systems to Global Perspectives Academic Press, Oxford. pp. 3-42

Wheat seed embryo excision enables the creation of axenic seedlings and Koch's postulates testing of putative bacterial endophytes

A - Papers appearing in refereed journals

Robinson, R. J., Fraaije, B. A., Clark, I. M., Jackson, R. W., Hirsch, P. R. and Mauchline, T. H. 2016. Wheat seed embryo excision enables the creation of axenic seedlings and Koch's postulates testing of putative bacterial endophytes. Scientific Reports. 6, p. 25581. https://doi.org/10.1038/srep25581

Novel European free-living, non-diazotrophic Bradyrhizobium isolates from contrasting soils that lack nodulation and nitrogen fixation genes - a genome comparison

A - Papers appearing in refereed journals

Jones, F. P., Clark, I. M., King, R., Shaw, L. J., Woodward, M. J. and Hirsch, P. R. 2016. Novel European free-living, non-diazotrophic Bradyrhizobium isolates from contrasting soils that lack nodulation and nitrogen fixation genes - a genome comparison. Scientific Reports. 6, p. 25858. https://doi.org/10.1038/srep25858

Soil pH determines microbial diversity and composition in the Park Grass Experiment

A - Papers appearing in refereed journals

Zhalnina, K., Dias, R., De Quadros, P. D., Davis-Richardson, A., Camargo, F. A. O., Clark, I. M., McGrath, S. P., Hirsch, P. R. and Triplett, E. W. 2015. Soil pH determines microbial diversity and composition in the Park Grass Experiment. Microbial Ecology. 69, pp. 395-406. https://doi.org/10.1007/s00248-014-0530-2

An analysis of Pseudomonas genomic diversity in take-all infected wheat fields reveals the lasting impact of wheat cultivars on the soil microbiota

A - Papers appearing in refereed journals

Mauchline, T. H., Chedom-Fotso, D., Chandra, G., Samuels, T., Greenaway, N., Backhaus, A., Mcmillan, V. E., Canning, G., Powers, S. J., Hammond-Kosack, K. E., Hirsch, P. R., Clark, I. M., Mehrabi, Z., Roworth, J., Burnell, J. and Malone, J. G. 2015. An analysis of Pseudomonas genomic diversity in take-all infected wheat fields reveals the lasting impact of wheat cultivars on the soil microbiota. Environmental Microbiology. 17 (11), pp. 4764-4778. https://doi.org/10.1111/1462-2920.13038

Over 150 years of long-term fertilization alters spatial scaling of microbial biodiversity

A - Papers appearing in refereed journals

Liang, Y., Wu, Liyou, Clark, I. M., Xue, K., Yang, Y., Van Nostrand, J. D., Deng, Y., He, Z., McGrath, S. P., Storkey, J., Hirsch, P. R., Sun, B. and Zhou, J. 2015. Over 150 years of long-term fertilization alters spatial scaling of microbial biodiversity. mBio. 6, pp. e00240-15. https://doi.org/10.1128/mBio.00240-15

The importance of the microbial N cycle in soil for crop plant nutrition

B - Book chapters etc edited externally

Hirsch, P. R. and Mauchline, T. H. 2015. The importance of the microbial N cycle in soil for crop plant nutrition. in: Sariaslani, S. and Gadd, G. M. (ed.) Advances in Applied Microbiology Vol.93 Elsevier.

Engineering soil organic matter quality: Biodiesel Co-Product (BCP) stimulates exudation of nitrogenous microbial biopolymers

A - Papers appearing in refereed journals

Redmile-Gordon, M. A., Evershed, R. P., Kuhl, A., Armenise, E., White, Robin P., Hirsch, P. R., Goulding, K. W. T. and Brookes, P. C. 2015. Engineering soil organic matter quality: Biodiesel Co-Product (BCP) stimulates exudation of nitrogenous microbial biopolymers. Geoderma. 259-260, pp. 205-212. https://doi.org/10.1016/j.geoderma.2015.06.006

Soil organic matter and the extracellular microbial matrix show contrasting responses to C and N availability

A - Papers appearing in refereed journals

Redmile-Gordon, M. A., Evershed, R. P., Hirsch, P. R., White, Robin P. and Goulding, K. W. T. 2015. Soil organic matter and the extracellular microbial matrix show contrasting responses to C and N availability. Soil Biology and Biochemistry. 88, pp. 257-267. https://doi.org/10.1016/j.soilbio.2015.05.025

The role of nodes in arsenic storage and distribution in rice

A - Papers appearing in refereed journals

Chen, Y., Moore, K. L., Miller, A. J., McGrath, S. P., Ma, J. F. and Zhao, F-J. 2015. The role of nodes in arsenic storage and distribution in rice. Journal of Experimental Botany. 66, pp. 3717-3724. https://doi.org/10.1093/jxb/erv164

Effects of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on abundance and activity of ammonia oxidizers in soil

A - Papers appearing in refereed journals

Florio, A., Clark, I., Hirsch, P. R., Jhurreea, D. and Benedetti, A. 2014. Effects of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on abundance and activity of ammonia oxidizers in soil. Biology And Fertility Of Soils. 50 (5), pp. 795-807. https://doi.org/10.1007/s00374-014-0897-8

The founding charter of the Genomic Observatories Network

A - Papers appearing in refereed journals

Davies, N., Field, D., Amaral-Zettler, L., Clark, M. S., Deck, J., Drummond, A., Faith, D. P., Geller, J., Gilbert, J., Glockner, F. O., Hirsch, P. R., Leong, J-A., Meyer, C., Obst, M., Planes, S., Scholin, C., Vogler, A. P., Gates, R. D., Toonen, R., Berteaux-Lecellier, V., Barbier, M., Barker, K., Bertilsson, S., Bicak, M., Bietz, M. J., Bobe, J., Bodrossy, L., Borja, A., Coddington, J., Fuhrman, J., Gerdts, G., Gillespie, R., Goodwin, K., Hanson, P. C., Hero, J-M., Hoekman, D., Jansson, J., Jeanthon, C., Kao, R., Klindworth, A., Knight, R., Kottmann, R., Koo, M. S., Kotoulas, G., Lowe, Andrew J., Marteinsson, V. T., Meyer, F., Morrison, N., Myrold, D. D., Pafilis, E., Parker, S., Parnell, J. J., Polymenakou, P. N., Ratnasingham, S., Roderick, G. K., Rodriguez-Ezpeleta, N., Schonrogge, K., Simon, N., Valette-Silver, N. J., Springer, Y. P., Stone, G. N., Stones-Havas, S., Sansone, S-A., Thibault, K. M., Wecker, P., Wichels, A., Wooley, J. C., Yahara, T. and Zingone, A. 2014. The founding charter of the Genomic Observatories Network. GigaScience. 3 (1), pp. 1-5. https://doi.org/10.1186/2047-217X-3-2

The impact of tissue type, growth stage and fertilizers on the community structure of cultured bacterial wheat endophytes in the Broadbalk experiment

C2 - Non-edited contributions to conferences

Robinson, R. R., Fraaije, B. A., Clark, I. M., Jackson, R. W., Hirsch, P. R. and Mauchline, T. H. 2014. The impact of tissue type, growth stage and fertilizers on the community structure of cultured bacterial wheat endophytes in the Broadbalk experiment. American Phytopathological Society (APS).

Brazilian Microbiome Project: revealing the unexplored microbial diversity - challenges and prospects

A - Papers appearing in refereed journals

Pylro, V. S., Roesch, L. F. W., Ortega, J. M., Do Amaral, A. M., Totola, M. R., Hirsch, P. R., Rosado, A. S., Goes-Neto, A., Da Costa Da Silva, A. L., Rosa, C. A., Morais, D. K., Andreote, F. D., Duarte, G. F., De Melo, I. S., Seldin, L., Lambais, M. R., Hungria, M., Peixoto, R. S., Kruger, R. H., Tsai, S. M. and Azevedo, V. 2014. Brazilian Microbiome Project: revealing the unexplored microbial diversity - challenges and prospects. Microbial Ecology. 67, pp. 237-241. https://doi.org/10.1007/s00248-013-0302-4

Data analysis for 16S microbial profiling from different benchtop sequencing platforms

A - Papers appearing in refereed journals

Pylro, V. S., Roesch, L. F. W., Morais, D. K., Clark, I. M., Hirsch, P. R. and Totola, M. R. 2014. Data analysis for 16S microbial profiling from different benchtop sequencing platforms. Journal of Microbiological Methods. 107 (December), pp. 30-37. https://doi.org/10.1016/j.mimet.2014.08.018

A combined cryo-scanning electron microscopy/cryoplaning approach to study the infection of Meloidogyne incognita eggs by Pochonia chlamydosporia

A - Papers appearing in refereed journals

Manzanilla-Lopez, R. H., Devonshire, J., Ward, E. and Hirsch, P. R. 2014. A combined cryo-scanning electron microscopy/cryoplaning approach to study the infection of Meloidogyne incognita eggs by Pochonia chlamydosporia. Nematology. 16, pp. 1059-1067. https://doi.org/10.1163/15685411-00002830

Quantifying the impact of microbes on soil structural development and behaviour in wet soils

A - Papers appearing in refereed journals

Helliwell, J. R., Miller, A. J., Whalley, W. R., Mooney, S. J. and Sturrock, C. J. 2014. Quantifying the impact of microbes on soil structural development and behaviour in wet soils. Soil Biology and Biochemistry. 74, pp. 138-147. https://doi.org/10.1016/j.soilbio.2014.03.009

Assessment of core and accessory genetic variation in Rhizobium leguminosarum symbiovar trifolii strains from diverse locations and host plants using PCR-based methods

A - Papers appearing in refereed journals

Mauchline, T. H., Hayat, R., Roberts, R., Powers, S. J. and Hirsch, P. R. 2014. Assessment of core and accessory genetic variation in Rhizobium leguminosarum symbiovar trifolii strains from diverse locations and host plants using PCR-based methods. Letters in Applied Microbiology. 59 (2), pp. 238-246. https://doi.org/10.1111/lam.12270

Measuring the soil-microbial interface: extraction of extracellular polymeric substances (EPS) from soil biofilms

A - Papers appearing in refereed journals

Redmile-Gordon, M. A., Brookes, P. C., Evershed, R. P., Goulding, K. W. T. and Hirsch, P. R. 2014. Measuring the soil-microbial interface: extraction of extracellular polymeric substances (EPS) from soil biofilms. Soil Biology and Biochemistry. 72 (May), pp. 163-171. https://doi.org/10.1016/j.soilbio.2014.01.025

Biodiesel co-product (BCP) decreases soil nitrogen (N) losses to groundwater

A - Papers appearing in refereed journals

Redmile-Gordon, M. A., Armenise, E., Hirsch, P. R. and Brookes, P. C. 2014. Biodiesel co-product (BCP) decreases soil nitrogen (N) losses to groundwater. Water Air And Soil Pollution. 225, p. 1831. https://doi.org/10.1007/s11270-013-1831-7

Effect of nitrogen supply form on the invasion of rice roots by the root-knot nematode, Meloidogyne graminicola

A - Papers appearing in refereed journals

Patil, J., Miller, A. J. and Gaur, H. 2013. Effect of nitrogen supply form on the invasion of rice roots by the root-knot nematode, Meloidogyne graminicola. Nematology. 15 (4), pp. 483-492. https://doi.org/10.1163/15685411-00002694

Bacterial endophytes in wheat (Triticum aestivum): isolation, characterisation and bio-prospecting

C2 - Non-edited contributions to conferences

Robinson, R., Mauchline, T. H., Hirsch, P. R., Jackson, R., Frost, H. and Fraaije, B. A. 2013. Bacterial endophytes in wheat (Triticum aestivum): isolation, characterisation and bio-prospecting. Schneider, C. (ed.) Abstracts 5th International Symposium on Plant Protection and Plant Health in Europe: Endophytes for Plant Protection: the State of the Art, Berlin, 26-29 May 2013 . DPG, Braunschweig. pp. 4-6

Effect of root nitrogen supply forms on attraction and repulsion of second-stage juveniles of Meloidogyne graminicola

A - Papers appearing in refereed journals

Patil, J., Powers, S. J., Davies, K. G., Gaur, H. S. and Miller, A. J. 2013. Effect of root nitrogen supply forms on attraction and repulsion of second-stage juveniles of Meloidogyne graminicola. Nematology. 15, pp. 469-482. https://doi.org/10.1163/15685411-00002697

A comparison of two colorimetric assays, based upon Lowry and Bradford techniques, to estimate total protein in soil extracts

A - Papers appearing in refereed journals

Redmile-Gordon, M. A., Armenise, E., White, Robin P., Hirsch, P. R. and Goulding, K. W. T. 2013. A comparison of two colorimetric assays, based upon Lowry and Bradford techniques, to estimate total protein in soil extracts. Soil Biology and Biochemistry. 67, pp. 166-173. https://doi.org/10.1016/j.soilbio.2013.08.017

Potential mineralization and nitrification in volcanic grassland soils in Chile

A - Papers appearing in refereed journals

Cardenas, L. M., Hatch, D. J., Scholefield, D., Jhurreea, D., Clark, I. M., Hirsch, P. R., Salazar, F., Rao-Ravella, S. and Alfaro, M. 2013. Potential mineralization and nitrification in volcanic grassland soils in Chile. Soil Science and Plant Nutrition. 59, pp. 380-391. https://doi.org/10.1080/00380768.2013.789395

Culture-independent molecular approaches to microbial ecology in soil and rhizosphere

B - Book chapters etc edited externally

Hirsch, P. R., Mauchline, T. H. and Clark, I. M. 2013. Culture-independent molecular approaches to microbial ecology in soil and rhizosphere. in: Debruijn, F. J. (ed.) Molecular microbial ecology of the rhizosphere, Volume 1 John Wiley & Sons Hoboken, N.J.. pp. 45-55

Do root exudates exert more influence on rhizosphere bacterial community structure than other rhizodeposits?

B - Book chapters etc edited externally

Hirsch, P. R., Miller, A. J. and Dennis, P. G. 2013. Do root exudates exert more influence on rhizosphere bacterial community structure than other rhizodeposits? in: Debruijn, F. J. (ed.) Molecular microbial ecology of the rhizosphere, Volume 1 John Wiley & Sons Hoboken, N.J.. pp. 229-242

Ca. Nitrososphaera and Bradyrhizobium are inversely correlated and related to agricultural practices in long-term field experiments

A - Papers appearing in refereed journals

Zhalnina, K., De Quadros, P. D., Gano, K. A., Davis-Richardson, A., Fagen, J. R., Brown, C. T., Giongo, A., Drew, J. C., Sayavedra-Soto, L. A., Arp, D. J., Camargo, F. A. O., Daroub, S. H., Clark, I. M., McGrath, S. P., Hirsch, P. R. and Triplett, E. W. 2013. Ca. Nitrososphaera and Bradyrhizobium are inversely correlated and related to agricultural practices in long-term field experiments. Frontiers in Microbiology. 4, p. 104. https://doi.org/10.3389/fmicb.2013.00104

Pochonia chlamydosporia: advances and challenges to improve its performance as a biological control agent of sedentary endo-parasitic nematodes

A - Papers appearing in refereed journals

Manzanilla-Lopez, R. H., Esteves, I., Finetti-Sialer, M. M., Hirsch, P. R., Ward, E., Devonshire, J. and Hidalgo-Diaz, L. 2013. Pochonia chlamydosporia: advances and challenges to improve its performance as a biological control agent of sedentary endo-parasitic nematodes. Journal of Nematology. 45 (1), pp. 1-7.

Transcriptional changes of the root-knot nematode Meloidogyne incognita in response to Arabidopsis thaliana root signals

A - Papers appearing in refereed journals

Teillet, A., Dybal, K., Kerry, B. R., Miller, A. J., Curtis, R. H. C. and Hedden, P. 2013. Transcriptional changes of the root-knot nematode Meloidogyne incognita in response to Arabidopsis thaliana root signals. PLOS ONE. 8, p. e61259. https://doi.org/10.1371/journal.pone.0061259

Light microscopy and cryo SEM observations on Pochonia chlamydosporia parasitism in root-knot nematodes

C2 - Non-edited contributions to conferences

Manzanilla-Lopez, R. H., Devonshire, J., Ward, E. and Hirsch, P. R. 2012. Light microscopy and cryo SEM observations on Pochonia chlamydosporia parasitism in root-knot nematodes. Abstracts 31st International Symposium of the European Society of Nematologists, Adana, Turkey, 23-27 September 2012 . pp. 215

Structure, fluctuation and magnitude of a natural grassland soil metagenome

A - Papers appearing in refereed journals

Delmont, T. O., Prestat, E., Keegan, K. P., Faubladier, M., Robe, P., Clark, I. M., Pelletier, E., Hirsch, P. R., Meyer, F., Gilbert, J. A., Le Paslier, D., Simonet, P. and Vogel, T. M. 2012. Structure, fluctuation and magnitude of a natural grassland soil metagenome. The ISME Journal. 6, pp. 1677-1687. https://doi.org/10.1038/ismej.2011.197

Roots, water and nutrient acquisition: let's get physical

A - Papers appearing in refereed journals

Chapman, N., Miller, A. J., Lindsey, K. and Whalley, W. R. 2012. Roots, water and nutrient acquisition: let's get physical. Trends in Plant Science. 17 (12), pp. 701-710. https://doi.org/10.1016/j.tplants.2012.08.001

Who's who in the plant root microbiome?

A - Papers appearing in refereed journals

Hirsch, P. R. and Mauchline, T. H. 2012. Who's who in the plant root microbiome? Nature Biotechnology. 30, pp. 961-962. https://doi.org/10.1038/nbt.2387

The Pochonia chlamydosporia serine protease gene vcp1 is subject to regulation by carbon, nitrogen and pH: implications for nematode biocontrol

A - Papers appearing in refereed journals

Ward, E., Kerry, B. R., Manzanilla-Lopez, R. H., Mutua, G., Devonshire, J., Kimenju, J. and Hirsch, P. R. 2012. The Pochonia chlamydosporia serine protease gene vcp1 is subject to regulation by carbon, nitrogen and pH: implications for nematode biocontrol. PLOS ONE. 7, p. e35657. https://doi.org/10.1371/journal.pone.0035657

Impacts of nitrogen application rates on the activity and diversity of denitrifying bacteria in the Broadbalk Wheat Experiment

A - Papers appearing in refereed journals

Clark, I. M., Buchkina, N., Jhurreea, D., Goulding, K. W. T. and Hirsch, P. R. 2012. Impacts of nitrogen application rates on the activity and diversity of denitrifying bacteria in the Broadbalk Wheat Experiment. Philosophical Transactions of the Royal Society B-Biological Sciences. 367, pp. 1235-1244. https://doi.org/10.1098/rstb.2011.0314

Pasteuria endospores from Heterodera cajani (Nematoda: Heteroderidae) exhibit inverted attachment and altered germination in cross-infection studies with Globodera pallida (Nematoda: Heteroderidae)

A - Papers appearing in refereed journals

Mohan, S., Mauchline, T. H., Rowe, J., Hirsch, P. R. and Davies, K. G. 2012. Pasteuria endospores from Heterodera cajani (Nematoda: Heteroderidae) exhibit inverted attachment and altered germination in cross-infection studies with Globodera pallida (Nematoda: Heteroderidae). FEMS Microbiology Ecology. 79 (3), pp. 675-684. https://doi.org/10.1111/j.1574-6941.2011.01249.x

Methylated arsenic species in plants originate from soil microorganisms

A - Papers appearing in refereed journals

Lomax, C., Liu, W-J., Wu, Liyou, Xue, K., Xiong, J., Zhou, J., McGrath, S. P., Meharg, A. A., Miller, A. J. and Zhao, F-J. 2012. Methylated arsenic species in plants originate from soil microorganisms. New Phytologist. 193 (3), pp. 665-672. https://doi.org/10.1111/j.1469-8137.2011.03956.x

A genomic network to monitor Earth

G - Articles in popular magazines and other technical publications

Davies, N., Field, D. and Hirsch, P. R. 2012. A genomic network to monitor Earth. Nature. 481 (7380), p. 145. https://doi.org/10.1038/481145a

Multi-scale approaches to investigating C dynamics in grassland systems

C2 - Non-edited contributions to conferences

Dungait, J. A. J., Bol, R., Murray, P. J., Dixon, E. R., Granger, S. J., Crotty, F. V., Hirsch, P. R., Evershed, R. and Goulding, K. W. T. 2011. Multi-scale approaches to investigating C dynamics in grassland systems. Abstracts AGU Fall Meeting 2011, San Francisco, 5-9 December 2011 .

Soil metagenomics reveals impacts of land management on microbial communities in long-term field experiments

C2 - Non-edited contributions to conferences

Hirsch, P. R. 2011. Soil metagenomics reveals impacts of land management on microbial communities in long-term field experiments. Abstracts 2011 International Conference on Soil Omics (ICSO), Nanjing, 19-24 November 2011 .

The role of soil bacteria in nitrogen cycling

C2 - Non-edited contributions to conferences

Hirsch, P. R. 2011. The role of soil bacteria in nitrogen cycling. Abstracts SEE11: Joint Meeting of the Royal Entomological Society of London and the Soil Ecology Society, Plymouth, 14-16 September 2011 .

Transporters involved in nitrogen uptake and movement

B - Book chapters etc edited externally

Miller, A. J. and Chapman, N. 2011. Transporters involved in nitrogen uptake and movement. in: Hawkesford, M. J. and Barraclough, P. B. (ed.) Molecular and physiological basis of nutrient use efficiency in crops John Wiley & Sons Chichester, West Sussex. pp. 193-210

Identification of new single nucleotide polymorphism-based markers for inter- and intraspecies discrimination of obligate bacterial parasites (Pasteuria spp.) of invertebrates

A - Papers appearing in refereed journals

Mauchline, T. H., Knox, R., Mohan, S., Powers, S. J., Kerry, B. R., Davies, K. G. and Hirsch, P. R. 2011. Identification of new single nucleotide polymorphism-based markers for inter- and intraspecies discrimination of obligate bacterial parasites (Pasteuria spp.) of invertebrates. Applied and Environmental Microbiology. 77, pp. 6388-6394. https://doi.org/10.1128/AEM.05185-11

Ecology of Pochonia chlamydosporia in the rhizosphere at the population, whole organism and molecular scales

B - Book chapters etc edited externally

Kerry, B. R. and Hirsch, P. R. 2011. Ecology of Pochonia chlamydosporia in the rhizosphere at the population, whole organism and molecular scales. in: Davies, K. G. and Spiegel, Y. (ed.) Biological control of plant-parasitic nematodes: building coherence between microbial ecology and molecular mechanisms Springer, Dordrecht. pp. 171-182

Developmental pattern of aquaporin expression in barley (Hordeum vulgare L.) leaves

A - Papers appearing in refereed journals

Besse, M., Knipfer, T., Miller, A. J., Verdeil, J-L., Jahn, T. P. and Fricke, W. 2011. Developmental pattern of aquaporin expression in barley (Hordeum vulgare L.) leaves. Journal of Experimental Botany. 62, pp. 4127-4142. https://doi.org/10.1093/jxb/err175

Water supply and not nitrate concentration determines primary root growth in Arabidopsis

A - Papers appearing in refereed journals

Chapman, N., Whalley, W. R., Lindsey, K. and Miller, A. J. 2011. Water supply and not nitrate concentration determines primary root growth in Arabidopsis. Plant, Cell and Environment. 34 (10), pp. 1630-1638. https://doi.org/10.1111/j.1365-3040.2011.02358.x

Pochonia chlamydosporia: advances and challenges to improve its performance as a biological control agent of sedentary endo-parasitic nematodes

C2 - Non-edited contributions to conferences

Manzanilla-Lopez, R. H., Esteves, I., Finetti-Sialer, M., Hirsch, P. R., Ward, E., Devonshire, J., Hidalgo, L. and Kerry, B. R. 2011. Pochonia chlamydosporia: advances and challenges to improve its performance as a biological control agent of sedentary endo-parasitic nematodes. Abstracts 50th Annual Meeting of the Society of Nematologists, Corvallis, 17-20 July 2011 . pp. 105-106

Exploring competitiveness and genetic variation in the nematophagous fungus Pochonia chlamydosporia var. chlamydosporia and its significance for biological control

C1 - Edited contributions to conferences/learned societies

Manzanilla-Lopez, R. H., Clark, I. M., Atkins, S. D., Hirsch, P. R. and Kerry, B. R. 2011. Exploring competitiveness and genetic variation in the nematophagous fungus Pochonia chlamydosporia var. chlamydosporia and its significance for biological control.

Transcriptome analysis shows differential gene expression in the saprotrophic to parasitic transition of Pochonia chlamydosporia

A - Papers appearing in refereed journals

Rosso, L. C., Finetti-Sialer, M. M., Hirsch, P. R., Ciancio, A., Kerry, B. R. and Clark, I. M. 2011. Transcriptome analysis shows differential gene expression in the saprotrophic to parasitic transition of Pochonia chlamydosporia. Applied Microbiology and Biotechnology. 90, pp. 1981-1994. https://doi.org/10.1007/s00253-011-3282-7

Development of a real-time PCR assay for detection and quantification of Rhizobium leguminosarum bacteria and discrimination between different biovars in zinc-contaminated soil

A - Papers appearing in refereed journals

Macdonald, C. A., Clark, I. M., Hirsch, P. R., Zhao, F-J. and McGrath, S. P. 2011. Development of a real-time PCR assay for detection and quantification of Rhizobium leguminosarum bacteria and discrimination between different biovars in zinc-contaminated soil. Applied and Environmental Microbiology. 77, pp. 4626-4633. https://doi.org/10.1128/AEM.02232-10

Impacts of land use change on denitrifying bacteria and nitrous oxide emissions

C2 - Non-edited contributions to conferences

Hirsch, P. R., Jhurreea, D., Scott, T., Misselbrook, T. H., Goulding, K. W. T. and Clark, I. M. 2011. Impacts of land use change on denitrifying bacteria and nitrous oxide emissions. Abstracts Theo Murphy Scientific Meeting: Nitrous Oxide, the Forgotten Greenhouse Gas, Chicheley, 23-24 May 2011 .

Spatial expression and regulation of rice high-affinity nitrate transporters by nitrogen and carbon status

A - Papers appearing in refereed journals

Feng, H., Yan, M., Fan, X., Li, B., Shen, Q., Miller, A. J. and Xu, G. 2011. Spatial expression and regulation of rice high-affinity nitrate transporters by nitrogen and carbon status. Journal of Experimental Botany. 62, pp. 2319-2332. https://doi.org/10.1093/jxb/erq403

Nutrient regulation of parasitism genes in the nematophagous fungus Pochonia chlamydosporia

C2 - Non-edited contributions to conferences

Ward, E., Kerry, B. R., Manzanilla-Lopez, R. H. and Hirsch, P. R. 2011. Nutrient regulation of parasitism genes in the nematophagous fungus Pochonia chlamydosporia. Abstracts 26th Fungal Genetics Conference, Asilomar, 15-20 March 2011 . pp. 237

Long-term impacts of zinc and copper enriched sewage sludge additions on bacterial, archaeal and fungal communities in arable and grassland soils

A - Papers appearing in refereed journals

Macdonald, C. A., Clark, I. M., Zhao, F-J., Hirsch, P. R., Singh, B. K. and McGrath, S. P. 2011. Long-term impacts of zinc and copper enriched sewage sludge additions on bacterial, archaeal and fungal communities in arable and grassland soils. Soil Biology and Biochemistry. 43, pp. 932-941. https://doi.org/10.1016/j.soilbio.2011.01.004

Accessing the soil metagenome for studies of microbial diversity

A - Papers appearing in refereed journals

Delmont, T. O., Robe, P., Cecillon, S., Clark, I. M., Constancias, F., Simonet, P., Hirsch, P. R. and Vogel, T. M. 2011. Accessing the soil metagenome for studies of microbial diversity. Applied and Environmental Microbiology. 77 (4), pp. 1315-1324. https://doi.org/10.1128/AEM.01526-10

Soil management in relation to sustainable agriculture and ecosystem services

A - Papers appearing in refereed journals

Powlson, D. S., Gregory, P. J., Whalley, W. R., Quinton, J. N., Hopkins, D. W., Whitmore, A. P., Hirsch, P. R. and Goulding, K. W. T. 2011. Soil management in relation to sustainable agriculture and ecosystem services. Food Policy. 36, pp. S72-S87. https://doi.org/10.1016/j.foodpol.2010.11.025

Transport systems for NO3- and NH4+

B - Book chapters etc edited externally

Orsel, M. and Miller, A. J. 2011. Transport systems for NO3- and NH4+. in: Foyer, C. H. and Zhang, H. (ed.) Nitrogen metabolism in plants in the post-genomic era (Annual Plant Reviews Vol. 42) Blackwell Publishing Oxford. pp. 83-102

Nitrate transporters and root architecture

B - Book chapters etc edited externally

Chapman, N. and Miller, A. J. 2011. Nitrate transporters and root architecture. in: Geisler, M. and Venema, K. (ed.) Transporters and pumps in plant signaling Springer, Berlin. pp. 165-190

Short-term effects of coumarin along the maize primary root axis

A - Papers appearing in refereed journals

Lupini, A., Sorgon, A., Miller, A. J. and Abenavoli, M. R. 2010. Short-term effects of coumarin along the maize primary root axis. Plant Signaling and Behavior. 5 (11), pp. 1395-400. https://doi.org/10.4161/psb.5.11.13021

Soil without plants: the consequences for microorganisms and mesofauna

C1 - Edited contributions to conferences/learned societies

Goulding, K. W. T., Murray, P. J., Sohi, S. P., Gilliam, L. M., Williams, J. K., Clark, I. M. and Hirsch, P. R. 2010. Soil without plants: the consequences for microorganisms and mesofauna. Gilkes, R. and Prakongkep, N. (ed.) Proceedings 19th World Congress of Soil Science, Brisbane, 1-6 August 2010 . Australian Society of Soil Science, Warragul. pp. 1622-1625

Nutrient regulation of parasitism in Pochonia chlamydosporia

C2 - Non-edited contributions to conferences

Ward, E., Mutua, G., Manzanilla-Lopez, R. H., Kerry, B. R., Kimenju, J. and Hirsch, P. R. 2010. Nutrient regulation of parasitism in Pochonia chlamydosporia. Abstracts ONTA 42nd Annual Meeting, Quito, Ecuador, 3-8 October 2010 . pp. 40

Exploring microbial diversity - methods and meaning

B - Book chapters etc edited externally

Ogilvie, L. A. and Hirsch, P. R. 2010. Exploring microbial diversity - methods and meaning. in: Tepper, G. H. (ed.) Species diversity and extinction Nova Science Publishers, Hauppauge. pp. 24pp

Pochonia chlamydosporia: some biological and physiological aspects in the host-parasite relationship

C2 - Non-edited contributions to conferences

Manzanilla-Lopez, R. H., Kerry, B. R., Esteves, I., Ward, E. and Hirsch, P. R. 2010. Pochonia chlamydosporia: some biological and physiological aspects in the host-parasite relationship. Abstracts ONTA 42nd Annual Meeting, Quito, Ecuador, 3-8 October 2010 . pp. 10

Separating the effects of water and nitrate stress on root system architecture

C2 - Non-edited contributions to conferences

Chapman, N., Whalley, W. R., Miller, A. J. and Lindsey, K. 2010. Separating the effects of water and nitrate stress on root system architecture. Abstracts 15th International Workshop on Plant Membrane Biology, Adelaide, 19-24 September 2010 . pp. 61

Effects of long-term fertilization and liming on microbial functions and diversity in the grassland site at Rothamsted Research, UK

C2 - Non-edited contributions to conferences

Wu, Liyou, Liang, Y., Clark, I. M., Wue, K., Deng, Y., He, Z., Van Nostrand, J., Macdonald, C., Hirsch, P. R., McGrath, S. P. and Zhou, J. 2010. Effects of long-term fertilization and liming on microbial functions and diversity in the grassland site at Rothamsted Research, UK. Abstracts 13th International Symposium on Microbial Ecology, Seattle, 22-27 August 2010 .

Relative impact of soil, metal source and metal concentration on bacterial community structure and community tolerance

A - Papers appearing in refereed journals

Macdonald, C. A., Yang, X., Clark, I. M., Zhao, F-J., Hirsch, P. R. and McGrath, S. P. 2010. Relative impact of soil, metal source and metal concentration on bacterial community structure and community tolerance. Soil Biology and Biochemistry. 42, pp. 1408-1417. https://doi.org/10.1016/j.soilbio.2010.05.002

Targeting the active bacterial communities in soil pores

C2 - Non-edited contributions to conferences

Mccabe, D., Clark, I. M., Sohi, S., Shaw, E. and Hirsch, P. R. 2010. Targeting the active bacterial communities in soil pores. Abstracts 13th International Symposium on Microbial Ecology, Seattle, 22-27 August 2010 .

Effects of long-term fertilization on spatial distribution patterns of microbial functional genes from the Rothamsted Experimental site

C2 - Non-edited contributions to conferences

Liang, Y., Wu, Liyou, Clark, I. M., Xue, K., Deng, Y., He, Z., Hirsch, P. R., McGrath, S. P. and Zhou, J. 2010. Effects of long-term fertilization on spatial distribution patterns of microbial functional genes from the Rothamsted Experimental site. Abstracts 13th International Symposium on Microbial Ecology, Seattle, 22-27 August 2010 .

Long-term agricultural experiments reveal N influence on ammonia oxidizers and the predominance of CA. nitrososphaera

C2 - Non-edited contributions to conferences

Giongo, A., Gano,K., Roesch, L., Casella, G., Daroub, S., Ramiez, K., Sayavedra-Soto, L., Arp, D., Clark, I. M., Hirsch, P. R., McGrath, S. P. and Triplett, E. 2010. Long-term agricultural experiments reveal N influence on ammonia oxidizers and the predominance of CA. nitrososphaera. Abstracts 13th International Symposium on Microbial Ecology, Seattle, 22-27 August 2010 .

Properties and physiological roles of plant potassium channels activated by reactive oxygen species

C2 - Non-edited contributions to conferences

Demidchik, V., Cuin, T. A., Svistunenko, D., Shabala, S., Smith, S. J., Miller, A. J., Sokolik, A. and Yurin, V. 2010. Properties and physiological roles of plant potassium channels activated by reactive oxygen species. Abstracts 15th International Workshop on Plant Membrane Biology, Adelaide, 19-24 September 2010 . pp. 55

Soil metagenomics: can we access the diversity?

C2 - Non-edited contributions to conferences

Delmont, T., Cecillon, S., Clark, I. M., Simonet, P., Hirsch, P. R. and Vogel, T. 2010. Soil metagenomics: can we access the diversity? Abstracts 13th International Symposium on Microbial Ecology, Seattle, 22-27 August 2010 .

Arabidopsis root K+-efflux conductance activated by hydroxyl radicals: single-channel properties, genetic basis and involvement in stress-induced cell death

A - Papers appearing in refereed journals

Demidchik, V., Cuin, T. A., Svistunenko, D., Smith, S. J., Miller, A. J., Shabala, S., Sokolik, A. and Yurin, V. 2010. Arabidopsis root K+-efflux conductance activated by hydroxyl radicals: single-channel properties, genetic basis and involvement in stress-induced cell death. Journal Of Cell Science. 123, pp. 1468-1479. https://doi.org/10.1242/jcs.064352

Plant nitrogen nutrition and transport

G - Articles in popular magazines and other technical publications

Miller, A. J. 2010. Plant nitrogen nutrition and transport. https://doi.org/10.1002/9780470015902.a0021257

A method for release and multiple strand amplification of small quantities of DNA from endospores of the fastidious bacterium Pasteuria penetrans

A - Papers appearing in refereed journals

Mauchline, T. H., Mohan, S., Davies, K. G., Schaff, J. E., Opperman, C. H., Kerry, B. R. and Hirsch, P. R. 2010. A method for release and multiple strand amplification of small quantities of DNA from endospores of the fastidious bacterium Pasteuria penetrans. Letters in Applied Microbiology. 50 (5), pp. 515-521. https://doi.org/10.1111/j.1472-765X.2010.02830.x

Culture-independent molecular techniques for soil microbial ecology

A - Papers appearing in refereed journals

Hirsch, P. R., Mauchline, T. H. and Clark, I. M. 2010. Culture-independent molecular techniques for soil microbial ecology. Soil Biology and Biochemistry. 42 (6), pp. 878-887. https://doi.org/10.1016/j.soilbio.2010.02.019

Are root exudates more important than other sources of rhizodeposits in structuring rhizosphere bacterial communities?

A - Papers appearing in refereed journals

Dennis, P. G., Miller, A. J. and Hirsch, P. R. 2010. Are root exudates more important than other sources of rhizodeposits in structuring rhizosphere bacterial communities? FEMS Microbiology Ecology. 72 (3), pp. 313-327. https://doi.org/10.1111/j.1574-6941.2010.00860.x

Role of antecedent soil moisture on microbial populations involved in gaseous N2O emissions - use of isotopomers to understand sources

C2 - Non-edited contributions to conferences

Cardenas, L. M., Bol, R., Bergstermann, A., Meijide, A., Hirsch, P. R., Jhurreea, D., Clark, I. M., Gilliam, L., Goulding, K. W. T., Well, R. and Scholefield, D. 2010. Role of antecedent soil moisture on microbial populations involved in gaseous N2O emissions - use of isotopomers to understand sources. Abstracts Stable Isotope Mass-Spectrometry User Group (SIMSUG) Meeting, Exeter, 28-30 April 2010 .

Plant homologs of the Plasmodium falciparum chloroquine-resistance transporter, Pf CRT, are required for glutathione homeostasis and stress responses

A - Papers appearing in refereed journals

Maughan, S. C., Pasternak, M., Cairns, N., Kiddle, G., Brach, T., Jarvis, R., Haas, F., Nieuwland, J., Lim, B., Müller, C., Salcedo-Sora, E., Kruse, C., Orsel, M., Hell, R., Miller, A. J., Bray, P., Foyer, C. H., Murray, J. A. H., Meyer, A. J. and Cobbett, C. S. 2010. Plant homologs of the Plasmodium falciparum chloroquine-resistance transporter, Pf CRT, are required for glutathione homeostasis and stress responses. Proceedings of the National Academy of Sciences. 107, pp. 2331-2336. https://doi.org/10.1073/pnas.0913689107

Are long-term changes in soil organic matter reflected in the soil communities?

C2 - Non-edited contributions to conferences

Murray, P. J., Hirsch, P. R., Gilliam, L. M., Sohi, S. P., Williams, J. K., Clark, I. M. and Brookes, P. C. 2009. Are long-term changes in soil organic matter reflected in the soil communities?

Are long-term changes in soil organic matter reflected in the soil communities?

C2 - Non-edited contributions to conferences

Brookes, P. C., Hirsch, P. R., Gilliam, L. M., Clark, I. M., Sohi, S. P., Williams, J. K. and Murray, P. J. 2009. Are long-term changes in soil organic matter reflected in the soil communities? Abstracts 2009 International Annual Meetings of ASA-CSSA-SSSA: Footprints in the Landscape: Sustainability through Plant and Soil Sciences, Pittsburgh, 1-5 November 2009 .

Are long-term changes in soil organic matter reflected in the soil communities?

C2 - Non-edited contributions to conferences

Murray, P. J., Hirsch, P. R., Gilliam, L. M., Sohi, S. P., Williams, J. K., Clark, I. M. and Brookes, P. C. 2009. Are long-term changes in soil organic matter reflected in the soil communities? Abstracts Forming Bridges in a Changing World: Society of Nematologists and Soil Ecology Society Conference, Burlington, 12-15 July 2009 .

A rapid method for release and multiple strand amplification of small quantities of DNA from Pasteuria penetrans endospores

C2 - Non-edited contributions to conferences

Mauchline, T. H., Mohan, S., Davies, K. G., Kerry, B. R. and Hirsch, P. R. 2009. A rapid method for release and multiple strand amplification of small quantities of DNA from Pasteuria penetrans endospores. Abstracts AAB Conference on Advances in Nematology, London, 15 December 2009 .

Multilevel analysis of primary metabolism provides new insights into the role of potassium nutrition for glycolysis and nitrogen assimilation in Arabidopsis roots

A - Papers appearing in refereed journals

Armengaud, P., Sulpice, R., Miller, A. J., Stitt, M., Amtmann, A. and Gibon, Y. 2009. Multilevel analysis of primary metabolism provides new insights into the role of potassium nutrition for glycolysis and nitrogen assimilation in Arabidopsis roots. Plant Physiology. 150, pp. 772-785. https://doi.org/10.1104/pp.108.133629

Through the keyhole: a key to open the bacterial lock; aka using BrdU to target pore space diversity

C2 - Non-edited contributions to conferences

Mccabe, D., Clark, I. M., Sohi, S. P., Shaw, L. and Hirsch, P. R. 2009. Through the keyhole: a key to open the bacterial lock; aka using BrdU to target pore space diversity. Abstracts Micro Soil: Integrating Biological, Physical and Chemical Techniques for the Study of Soil Micro-Habitats, Dundee, 16-17 September 2009 . pp. 24-25

Microbial diversity maintained in soils starved of plant inputs for 50 years

C2 - Non-edited contributions to conferences

Clark, I. M., Gilliam, L. M., Sohi, S. P., Williams, J., Murray, P. J. and Hirsch, P. R. 2009. Microbial diversity maintained in soils starved of plant inputs for 50 years. Abstracts BAGECO 10: Bacterial Genetics and Ecology - Coexisting on a Changing Planet, Uppsala, 15-19 June 2009 . pp. 119

What can we learn from the Rothamsted Classical Experiments using molecular techniques

C2 - Non-edited contributions to conferences

Hirsch, P. R. 2009. What can we learn from the Rothamsted Classical Experiments using molecular techniques. Abstracts UK-Brazil Workshop on Soil Quality and Soil Processes, Rio de Janeiro, 20-27 March 2009 . pp. 13

Sampling bacteria at the microhabitat scale

C2 - Non-edited contributions to conferences

Hirsch, P. R., Dennis, P. G. and Miller, A. J. 2009. Sampling bacteria at the microhabitat scale. Abstracts Micro Soil: Integrating Biological, Physical and Chemical Techniques for the Study of Soil Micro-Habitats, Dundee, 16-17 September 2009 . pp. 9

What plants do for soil and what happens after they've gone

C2 - Non-edited contributions to conferences

Hirsch, P. R. and Clark, I. M. 2009. What plants do for soil and what happens after they've gone. Abstracts Plant Soil Interactions: Joint Meeting of the Scottish Root Group and the Scottish Discussion Group of the British Society of Soil Science, Aberdeen, 3 November 2009 .

Sequencing the microbial metagenome of an iconic grassland soil, the Rothamsted Park Grass Experiment

C2 - Non-edited contributions to conferences

Hirsch, P. R. and Clark, I. M. 2009. Sequencing the microbial metagenome of an iconic grassland soil, the Rothamsted Park Grass Experiment. Abstracts BAGECO 10: Bacterial Genetics and Ecology - Coexisting on a Changing Planet, Uppsala, 15-19 June 2009 . pp. 73

The impact of nutrition on parasitism of nematode eggs by Pochonia chlamydosporia

C2 - Non-edited contributions to conferences

Mutua, G., Kerry, B. R., Hirsch, P. R., Kimenju, J., Manzanilla-Lopez, R. H. and Ward, E. 2009. The impact of nutrition on parasitism of nematode eggs by Pochonia chlamydosporia. Abstracts BSPP Presidential Meeting. Darwin to Disease: Crops and their Pathogens, Oxford 21-22 september 2009 .

Electrophysiological characterization of pathways for K+ uptake into growing and non-growing leaf cells of barley

A - Papers appearing in refereed journals

Volkow, V., Boscari, A., Clement, M., Miller, A. J., Amtmann, A. and Fricke, W. 2009. Electrophysiological characterization of pathways for K+ uptake into growing and non-growing leaf cells of barley. Plant, Cell and Environment. 32 (12), pp. 1778-1790. https://doi.org/10.1111/j.1365-3040.2009.02034.x

Advantages of the metagenomic approach for soil exploration: reply from Vogel et al

A - Papers appearing in refereed journals

Vogel, T. M., Hirsch, P. R., Simonet, P., Jansson, J. K., Tiedje, J. M., Van Elsas, J. D., Nalin, R., Philippot, L. and Bailey, M. J. 2009. Advantages of the metagenomic approach for soil exploration: reply from Vogel et al. Nature Reviews Microbiology. 7, pp. 756-757. https://doi.org/10.1038/nrmicro2119-c3

Potassium channels in barley: cloning, functional characterization and expression analyses in relation to leaf growth and development

A - Papers appearing in refereed journals

Boscari, A., Clement, M., Volkov, V., Golldack, D., Hybiak, J., Miller, A. J., Amtmann, A. and Fricke, W. 2009. Potassium channels in barley: cloning, functional characterization and expression analyses in relation to leaf growth and development. Plant, Cell and Environment. 32 (12), pp. 1761-1777. https://doi.org/10.1111/j.1365-3040.2009.02033.x

Starving the soil of plant inputs for 50 years reduces abundance but not diversity of soil bacterial communities

A - Papers appearing in refereed journals

Hirsch, P. R., Gilliam, L. M., Sohi, S. P., Williams, J. K., Clark, I. M. and Murray, P. J. 2009. Starving the soil of plant inputs for 50 years reduces abundance but not diversity of soil bacterial communities. Soil Biology and Biochemistry. 41 (9), pp. 2021-2024. https://doi.org/10.1016/j.soilbio.2009.07.011

Freeways in the plant: transporters for N, P and S and their regulation

A - Papers appearing in refereed journals

Miller, A. J., Shen, Q. and Xu, G. 2009. Freeways in the plant: transporters for N, P and S and their regulation. Current Opinion in Plant Biology. 12, pp. 284-290. https://doi.org/10.1016/j.pbi.2009.04.010

Use of real-time quantitative PCR to investigate root and gall colonisation by co-inoculated isolates of the nematophagous fungus Pochonia chlamydosporia

A - Papers appearing in refereed journals

Atkins, S. D., Peteira, B., Clark, I. M., Kerry, B. R. and Hirsch, P. R. 2009. Use of real-time quantitative PCR to investigate root and gall colonisation by co-inoculated isolates of the nematophagous fungus Pochonia chlamydosporia. Annals of Applied Biology - AAB. 155, pp. 143-152. https://doi.org/10.1111/j.1744-7348.2009.00333.x

Caracterizacion del hongo nematofago cepa IMI SD 187 de Pochonia chlamydosporia var. catenulata (Kamyscho ex Barron y Onions) Zare y Gams

C2 - Non-edited contributions to conferences

Peteira, B., Hidalgo, L., Esteves, I., Montes De Oca, N., Atkins, S. D., Hirsch, P. R. and Kerry, B. R. 2009. Caracterizacion del hongo nematofago cepa IMI SD 187 de Pochonia chlamydosporia var. catenulata (Kamyscho ex Barron y Onions) Zare y Gams.

Measuring abundance, diversity and parasitic ability in two populations of the nematophagous fungus Pochonia chlamydosporia var. chlamydosporia

A - Papers appearing in refereed journals

Manzanilla-Lopez, R. H., Atkins, S. D., Clark, I. M., Kerry, B. R. and Hirsch, P. R. 2009. Measuring abundance, diversity and parasitic ability in two populations of the nematophagous fungus Pochonia chlamydosporia var. chlamydosporia. Biocontrol Science and Technology. 19, pp. 391-406. https://doi.org/10.1080/09583150902783793

Exploring competitiveness and genetic variation in the nematophagous fungus Pochonia chlamydosporia var. chlamydosporia and its significance for biological control

C2 - Non-edited contributions to conferences

Manzanilla-Lopez, R. H., Clark, I. M., Atkins, S., Hirsch, P. R. and Kerry, B. R. 2009. Exploring competitiveness and genetic variation in the nematophagous fungus Pochonia chlamydosporia var. chlamydosporia and its significance for biological control. Abstracts IOBC 5th Meeting on Multitrophic Interactions in Soil, Uppsala, 11-13 June 2009 . pp. 27

TerraGenome: a consortium for the sequencing of a soil metagenome

A - Papers appearing in refereed journals

Vogel, T. M., Simonet, P., Jansson, J. K., Hirsch, P. R., Tiedje, J. M., Van Elsas, J. D., Bailey, M. J., Nalin, R. and Philippot, L. 2009. TerraGenome: a consortium for the sequencing of a soil metagenome. Nature Reviews Microbiology. 7, p. 252. https://doi.org/10.1038/nrmicro2119

Rapid and reliable DNA extraction and PCR fingerprinting methods to discriminate multiple biotypes of the nematophagous fungus Pochonia chlamydosporia isolated from plant rhizospheres

A - Papers appearing in refereed journals

Manzanilla-Lopez, R. H., Clark, I. M., Atkins, S. D., Hirsch, P. R. and Kerry, B. R. 2009. Rapid and reliable DNA extraction and PCR fingerprinting methods to discriminate multiple biotypes of the nematophagous fungus Pochonia chlamydosporia isolated from plant rhizospheres. Letters in Applied Microbiology. 48 (1), pp. 71-76. https://doi.org/10.1111/j.1472-765X.2008.02489.x

Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different functions and kinetic properties in uptake and translocation

A - Papers appearing in refereed journals

Ai, P., Sun, S., Zhao, J., Fan, X., Xin, W., Guo, Q., Yu, L., Shen, Q., Wu, P., Miller, A. J. and Xu, G. 2009. Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different functions and kinetic properties in uptake and translocation. The Plant Journal. 57, pp. 798-809. https://doi.org/10.1111/j.1365-313X.2008.03726.x

Linking rhizoplane pH and bacterial density at the microhabitat scale

A - Papers appearing in refereed journals

Dennis, P. G., Hirsch, P. R., Smith, S. J., Taylor, R. G., Valsami-Jones, E. and Miller, A. J. 2009. Linking rhizoplane pH and bacterial density at the microhabitat scale. Journal of Microbiological Methods. 76, pp. 101-104. https://doi.org/10.1016/j.mimet.2008.09.013

Long-term impacts of metal-rich sludge additions on soil microbial communities - effects of metal source and soil type

C2 - Non-edited contributions to conferences

Macdonald, C., Yang, X., Clark, I. M., Hirsch, P. R. and McGrath, S. P. 2008. Long-term impacts of metal-rich sludge additions on soil microbial communities - effects of metal source and soil type. Abstracts 12th International Symposium on Microbial Ecology (ISME12): Microbial Diversity - Sustaining the Blue Planet, Cairns, 17-22 August 2008 .

A putative role for the plasma membrane potential in the control of the expression of the gene encoding the tomato high-affinity potassium transporter HAK5

A - Papers appearing in refereed journals

Nieves-Cordones, M., Miller, A. J., Aleman, F., Martinez, V. and Rubio, F. 2008. A putative role for the plasma membrane potential in the control of the expression of the gene encoding the tomato high-affinity potassium transporter HAK5. Plant Molecular Biology. 68, pp. 521-532. https://doi.org/10.1007/s11103-008-9388-3

Interactions on roots and the efficacy of Pochonia chlamydosporia as a biological control agent

C2 - Non-edited contributions to conferences

Kerry, B. R., Clark, I. M., Esteves, I., Finetti, M., Hirsch, P. R., Gomez-Gil, L., Manzanilla-Lopez, R. H. and Rosso, L. C. 2008. Interactions on roots and the efficacy of Pochonia chlamydosporia as a biological control agent. Abstracts 6th Seminario Cientifico Internacional de Sanidad Vegetal, Habana, 22-26 September 2008 .

Cloning and expression analyses of potassium channels in barley leaves

C2 - Non-edited contributions to conferences

Fricke, W., Volkov, V., Boscari, A., Clement, M., Amtmann, A., Golldack, D. and Miller, A. J. 2008. Cloning and expression analyses of potassium channels in barley leaves. https://doi.org/10.1016/j.cbpa.2008.04.524

New approaches to an old problem: linking soil quality and microbial communities

C2 - Non-edited contributions to conferences

Hirsch, P. R. 2008. New approaches to an old problem: linking soil quality and microbial communities. Abstracts BSSS Autumn Meeting on Something Old, Something New: Integrating Molecular Biology with Other Techniques in Soil Science, Harpenden, 17 September 2008 .

A novel family of plastid transporters involved in glutathione metabolism

C2 - Non-edited contributions to conferences

Cobbett, C., Maughan, S., Kiddle, G., Cairns, N., Jarvis, R., Nieuwland, J., Pasternak, M., Lim, B., Miller, A. J., Murray, J., Foyer, C. H. and Meyer, A. 2008. A novel family of plastid transporters involved in glutathione metabolism. Abstracts International Symposium on Glutathione and Related Thiols in Microorganisms and Plants, Nancy, 26-29 August 2008 .

Bacterial diversity of the Broadbalk 'Classical' winter wheat experiment in relation to long-term fertilizer inputs

A - Papers appearing in refereed journals

Ogilvie, L. A., Hirsch, P. R. and Johnston, A. W. B. 2008. Bacterial diversity of the Broadbalk 'Classical' winter wheat experiment in relation to long-term fertilizer inputs. Microbial Ecology. 56, pp. 525-537. https://doi.org/10.1007/s00248-008-9372-0

A novel method for sampling bacteria on plant root and soil surfaces at the microhabitat scale

A - Papers appearing in refereed journals

Dennis, P. G., Miller, A. J., Clark, I. M., Taylor, R. G., Valsami-Jones, E. and Hirsch, P. R. 2008. A novel method for sampling bacteria on plant root and soil surfaces at the microhabitat scale. Journal of Microbiological Methods. 75, pp. 12-18. https://doi.org/10.1016/j.mimet.2008.04.013

Gene expression profiles in Pochonia chlamydosporia in conditions of saprotrophic-to-parasite transition

C2 - Non-edited contributions to conferences

Finetti-Sialer, M., Hirsch, P. R., Kerry, B. R. and Clark, I. M. 2008. Gene expression profiles in Pochonia chlamydosporia in conditions of saprotrophic-to-parasite transition. Abstracts 5th International Congress of Nematology, Brisbane, 13-18 July 2008 .

Two rice nitrate transporters encoded by one gene that each show differential regulation by pH

C2 - Non-edited contributions to conferences

Fan, X., Yan, M., Li, B., Xu, G., Shen, Q. and Miller, A. J. 2008. Two rice nitrate transporters encoded by one gene that each show differential regulation by pH. Abstracts SEB Plant Transport Group Meeting, Manchester, 4-5 September 2008 .

Ecology of Pochonia chlamydosporia in the rhizosphere

C2 - Non-edited contributions to conferences

Kerry, B. R. and Hirsch, P. R. 2008. Ecology of Pochonia chlamydosporia in the rhizosphere. Abstracts 5th International Congress of Nematology, Brisbane, 13-18 July 2008 . pp. 153

Investigating glutamate receptor-like gene co-expression in Arabidopsis thaliana

A - Papers appearing in refereed journals

Roy, S. J., Gilliham, M., Berger, B., Essah, P. A., Cheffings, C., Miller, A. J., Davenport, R. J., Liu, L-H., Skynner, M. J., Davies, J. M., Richardson, P., Leigh, R. A. and Tester, M. 2008. Investigating glutamate receptor-like gene co-expression in Arabidopsis thaliana. Plant, Cell and Environment. 31 (6), pp. 861-871. https://doi.org/10.1111/j.1365-3040.2008.01801.x

Rhizobial symbioses - past, present and future

C2 - Non-edited contributions to conferences

Hirsch, P. R. 2008. Rhizobial symbioses - past, present and future. Abstracts Biological Nitrogen Fixation Convening, Seattle, 21-22 April 2008 .

Survival of bacterial DNA and culturable bacteria in archived soils from the Rothamsted Broadbalk experiment

A - Papers appearing in refereed journals

Clark, I. M. and Hirsch, P. R. 2008. Survival of bacterial DNA and culturable bacteria in archived soils from the Rothamsted Broadbalk experiment. Soil Biology and Biochemistry. 40, pp. 1090-1102. https://doi.org/10.1016/j.soilbio.2007.11.021

Book review: Plant solute transport. Yeo, A.R., Flowers, T.J. eds. 2007. Oxford: Wiley-Blackwell

G - Articles in popular magazines and other technical publications

Miller, A. J. 2008. Book review: Plant solute transport. Yeo, A.R., Flowers, T.J. eds. 2007. Oxford: Wiley-Blackwell. Annals of Botany. 101 (7), pp. 1050-1051. https://doi.org/10.1093/aob/mcn035

Cytosolic nitrate ion homeostasis: could it have a role in sensing nitrogen status?

A - Papers appearing in refereed journals

Miller, A. J. and Smith, S. J. 2008. Cytosolic nitrate ion homeostasis: could it have a role in sensing nitrogen status? Annals of Botany. 101, pp. 485-489. https://doi.org/10.1093/aob/mcm313

Amino acids and nitrate as signals for the regulation of nitrogen acquisition

A - Papers appearing in refereed journals

Miller, A. J., Fan, X., Shen, Q. and Smith, S. J. 2008. Amino acids and nitrate as signals for the regulation of nitrogen acquisition. Journal of Experimental Botany. 59, pp. 111-119. https://doi.org/10.1093/jxb/erm208

HvPIP1;6, a barley (Hordeum vulgare L.) plasma membrane water channel particularly expressed in growing compared with non-growing leaf tissues

A - Papers appearing in refereed journals

Wei, W., Alexandersson, E., Golldack, D., Miller, A. J., Kjellbom, P. O. and Fricke, W. 2007. HvPIP1;6, a barley (Hordeum vulgare L.) plasma membrane water channel particularly expressed in growing compared with non-growing leaf tissues. Plant and cell physiology. 48 (8), pp. 1132-1147. https://doi.org/10.1093/pcp/pcm083

Nitrate signalling and the two component high affinity uptake system in Arabidopsis (Addendum to: Characterisation of a two component high affinity nitrate uptake system in Arabidopsis: physiology and protein-protein interaction. Plant Physiology 2006; 142:1304-17)

A - Papers appearing in refereed journals

Orsel, M., Chopin, F., Leleu, O., Smith, S. J., Krapp, A., Daniel-Vedele, F. and Miller, A. J. 2007. Nitrate signalling and the two component high affinity uptake system in Arabidopsis (Addendum to: Characterisation of a two component high affinity nitrate uptake system in Arabidopsis: physiology and protein-protein interaction. Plant Physiology 2006; 142:1304-17). Plant Signaling and Behavior. 2, pp. 260-262.

Nitrate transport and signalling

A - Papers appearing in refereed journals

Miller, A. J., Fan, X., Orsel, M., Smith, S. J. and Wells, D. M. 2007. Nitrate transport and signalling. Journal of Experimental Botany. 58, pp. 2297-2306. https://doi.org/10.1093/jxb/erm066

The Arabidopsis ATNRT2.7 nitrate transporter controls nitrate content in seeds

A - Papers appearing in refereed journals

Chopin, F., Orsel, M., Dorbe, M-F., Chardon, F., Truong, H-N., Miller, A. J., Krapp, A. and Daniel-Vedele, F. 2007. The Arabidopsis ATNRT2.7 nitrate transporter controls nitrate content in seeds. The Plant Cell. 19, pp. 1590-1602. https://doi.org/10.1105/tpc.107.050542

Comparing nitrate storage and remobilization in two rice cultivars that differ in their nitrogen use efficiency

A - Papers appearing in refereed journals

Fan, X., Jia, L., Li, Y., Smith, S. J., Miller, A. J. and Shen, Q. 2007. Comparing nitrate storage and remobilization in two rice cultivars that differ in their nitrogen use efficiency. Journal of Experimental Botany. 58, pp. 1729-1740. https://doi.org/10.1093/jxb/erm033

A realistic appraisal of plant signals involved in nematode attraction to roots

C2 - Non-edited contributions to conferences

Perry, R. N. and Miller, A. J. 2007. A realistic appraisal of plant signals involved in nematode attraction to roots. Abstracts 18th Symposium of the Nematological Society of Southern Africa, Port Elizabeth, 6-10 May 2007 . pp. 44

An Arabidopsis low affinity nitrate transporter with a novel transport mechanism

C2 - Non-edited contributions to conferences

Orsel, M. and Miller, A. J. 2006. An Arabidopsis low affinity nitrate transporter with a novel transport mechanism. Abstracts SEB Plant Transport Group Meeting, Ashford, 4-6 September 2006 . pp. 15

Expression and functional analysis of rice NRT2 nitrate transporters

C2 - Non-edited contributions to conferences

Fan, X., Shen, Q., Smith, S. and Miller, A. J. 2006. Expression and functional analysis of rice NRT2 nitrate transporters. Abstracts SEB Plant Transport Group Meeting, Ashford, 4-6 September 2006 . pp. 31

Non-destructive micro-scale sampling of bacteria from a root surface using a tungsten rod with a standardised sampling area controlled by a micromanipulator

D1 - Technical reports: non-confidential

Dennis, P. G., Miller, A. J. and Hirsch, P. R. 2006. Non-destructive micro-scale sampling of bacteria from a root surface using a tungsten rod with a standardised sampling area controlled by a micromanipulator. Swiss Federal Research Institute, Birmensdorf.

Characterization of a two-component high-affinity nitrate uptake system in Arabidopsis. Physiology and protein-protein interaction

A - Papers appearing in refereed journals

Orsel, M., Chopin, F., Leleu, O., Smith, S. J., Krapp, A., Daniel-Vedele, F. and Miller, A. J. 2006. Characterization of a two-component high-affinity nitrate uptake system in Arabidopsis. Physiology and protein-protein interaction. Plant Physiology. 142, pp. 1304-1317. https://doi.org/10.1104/pp.106.085209

DNA fingerprinting methodology to monitor infectivity, competition and rhizosphere competence of Pochonia chlamydosporia isolates within bulk soil, rhizoshere and Meloidogyne incognita egg masses

C2 - Non-edited contributions to conferences

Manzanilla-Lopez, R. H., Clark, I. M., Atkins, S., Hirsch, P. R. and Kerry, B. R. 2006. DNA fingerprinting methodology to monitor infectivity, competition and rhizosphere competence of Pochonia chlamydosporia isolates within bulk soil, rhizoshere and Meloidogyne incognita egg masses. Abstracts AAB Conference on Advances in Nematology, London, 12 December 2006 .

Assessing genetic diversity of Pochonia chlamydosporia populations using ERIC primers

C2 - Non-edited contributions to conferences

Manzanilla-Lopez, R. H., Clark, I. M., Hirsch, P. R. and Kerry, B. R. 2006. Assessing genetic diversity of Pochonia chlamydosporia populations using ERIC primers. Abstracts 38th ONTA Annual Meeting, San Jose, 26-30 June 2006 .

Biological and chemical characterisation of microsites in the rhizosphere

C2 - Non-edited contributions to conferences

Dennis, P. G., Miller, A. J. and Hirsch, P. R. 2006. Biological and chemical characterisation of microsites in the rhizosphere. Abstracts Final Meeting of COST Action 631. From Understanding and Modelling to Application: Managing the Natural Potentials of the Rhizosphere for Designing Rhizosphere Technologies, Prague, 20-23 April 2006 . pp. 7

Novel method to investigate spatiotemporal trends in rhizosphere bacterial communities

C2 - Non-edited contributions to conferences

Dennis, P. G., Miller, A. J. and Hirsch, P. R. 2006. Novel method to investigate spatiotemporal trends in rhizosphere bacterial communities. Abstracts 158th Meeting of the Society for General Microbiology, Warwick, 3-6 April 2006 . pp. 52

Monitoring active bacterial communities in soil

C2 - Non-edited contributions to conferences

Clark, I. M., Cookson, W. R., Hampshire, D. and Hirsch, P. R. 2006. Monitoring active bacterial communities in soil. Abstracts 158th Meeting of the Society for General Microbiology, Warwick, 3-6 April 2006 . pp. 52

Differential regulation of the Chlamydomonas Nar1 gene family by carbon and nitrogen

A - Papers appearing in refereed journals

Mariscal, V., Moulin, P., Orsel, M., Miller, A. J., Fernandez, E. and Galvan, A. 2006. Differential regulation of the Chlamydomonas Nar1 gene family by carbon and nitrogen. Protist. 157, pp. 421-433. https://doi.org/10.1016/j.protis.2006.06.003

Electrochemical methods and measuring transmembrane ion gradients

B - Book chapters etc edited externally

Miller, A. J. and Wells, D. M. 2006. Electrochemical methods and measuring transmembrane ion gradients. in: Volkov, A. G. (ed.) Plant electrophysiology - theory and methods Springer, Berlin. pp. 15-34

Extracellular Ca2+ ameliorates NaC1-induced K+ loss from Arabidopsis root and leaf cells by controlling plasma membrane K+-permeable channels

A - Papers appearing in refereed journals

Shabala, S., Demidchik, V., Shabala, L., Cuin, T. A., Smith, S. J., Miller, A. J., Davies, J. M. and Newman, I. A. 2006. Extracellular Ca2+ ameliorates NaC1-induced K+ loss from Arabidopsis root and leaf cells by controlling plasma membrane K+-permeable channels. Plant Physiology. 141, pp. 1653-1665. https://doi.org/10.1104/pp.106.082388

The influence of season, agricultural management and soil properties on gross nitrogen transformations and bacterial community structure

A - Papers appearing in refereed journals

Cookson, W. R., Marschner, P., Clark, I. M., Milton, N., Smirk, M. N., Murphy, D. V., Osman, M., Stockdale, E. A. and Hirsch, P. R. 2006. The influence of season, agricultural management and soil properties on gross nitrogen transformations and bacterial community structure. Australian journal of soil research. 44, pp. 453-465. https://doi.org/10.1071/SR05042

Glutamine transport and feedback regulation of nitrate reductase activity in barley roots leads to changes in cytosolic nitrate pools

A - Papers appearing in refereed journals

Fan, X., Gordon-Weeks, R., Shen, Q. and Miller, A. J. 2006. Glutamine transport and feedback regulation of nitrate reductase activity in barley roots leads to changes in cytosolic nitrate pools. Journal of Experimental Botany. 57, pp. 1333-1340. https://doi.org/10.1093/jxb/erj110

Distribution of environmental mycobacteria in Karonga District, Northern Malawi

A - Papers appearing in refereed journals

Chilima, B. Z., Clark, I. M., Floyd, S., Fine, P. E. M. and Hirsch, P. R. 2006. Distribution of environmental mycobacteria in Karonga District, Northern Malawi. Applied and Environmental Microbiology. 72 (4), pp. 2343-2350. https://doi.org/10.1128/AEM.72.4.2343-2350.2006

The short-term growth response to salt of the developing barley leaf

A - Papers appearing in refereed journals

Fricke, W., Akhiyarova, G., Wei, W., Alexandersson, E., Miller, A. J., Kjellbom, P. O., Richardson, A., Wojciechowski, T., Schreiber, L., Veselov, D., Kudoyarova, G. and Volkov, V. 2006. The short-term growth response to salt of the developing barley leaf. Journal of Experimental Botany. 57, pp. 1079-1095. https://doi.org/10.1093/jxb/erj095

Life in earth: the impact of GM plants on soil ecology?

A - Papers appearing in refereed journals

Lilley, A. K., Bailey, M. J., Cartwright, C., Turner, S. L. and Hirsch, P. R. 2006. Life in earth: the impact of GM plants on soil ecology? Trends in Biotechnology. 24, pp. 9-14. https://doi.org/10.1016/j.tibtech.2005.11.005

A comparison of nitrate transport in four different rice (Oryza sativa L.) cultivars

A - Papers appearing in refereed journals

Fan, X., Shen, Q., Ma, Z., Zhu, H., Yin, X. and Miller, A. J. 2005. A comparison of nitrate transport in four different rice (Oryza sativa L.) cultivars. Science in China Series C-Life Sciences. 48(Special issue), pp. 897-911.

Real-time PCR to monitor and assess the efficacy of two types of inoculum of the nematophagous fungus Pochonia chlamydosporia var. catenulata against root-knot nematode populations in the field

A - Papers appearing in refereed journals

Peteira, B., Puertas, A., Hidalgo-Diaz, L., Hirsch, P. R., Kerry, B. R. and Atkins, S. D. 2005. Real-time PCR to monitor and assess the efficacy of two types of inoculum of the nematophagous fungus Pochonia chlamydosporia var. catenulata against root-knot nematode populations in the field. Biotecnologia Aplicada. 22, pp. 261-266.

Novel method to investigate spatiotemporal trends in bacterial communities in the rhizosphere

C2 - Non-edited contributions to conferences

Dennis, P. G., Miller, A. J. and Hirsch, P. R. 2005. Novel method to investigate spatiotemporal trends in bacterial communities in the rhizosphere. Abstracts 11th Annual UK Molecular Microbial Ecology Group Meeting (MMEG-11), Norwich, 11-12 July 2005 .

Growth zone of barley leaf possesses intrinsic water and ion transport properties

C2 - Non-edited contributions to conferences

Volkov, V., Boscari, A., Amtmann, A., Miller, A. J. and Fricke, W. 2005. Growth zone of barley leaf possesses intrinsic water and ion transport properties. Abstracts SEB Plant Transport Group Meeting, Glasgow, 7-9 September 2005 .

A two component high affinity nitrate uptake system in arabidopsis

C2 - Non-edited contributions to conferences

Orsel, M., Chopin, F., Smith, S., Krapp, A., Vedele, F. and Miller, A. J. 2005. A two component high affinity nitrate uptake system in arabidopsis. Abstracts SEB Plant Transport Group Meeting, Glasgow, 7-9 September 2005 .

The effect of glutamine on the cytosolic nitrate pools of barley roots

C1 - Edited contributions to conferences/learned societies

Fan, X., Gordon-Weeks, R., Shen, Q. and Miller, A. J. 2005. The effect of glutamine on the cytosolic nitrate pools of barley roots. Li, C. J. (ed.) Plant Nutrition for Food Security, Human Health and Environmental Protection. Proceedings 15th International Plant Nutrition Colloquium, Beijing, 14-19 September 2005 . Tsinghua University Press, Beijing. pp. 168-169

K+ inward-rectifying shaker-type channel in barley leaf potentially involved in leaf growth

C2 - Non-edited contributions to conferences

Boscari, A., Volkov, V., Wojciechowski, T., Richardson, A., Antmann, A., Miller, A. J. and Fricke, W. 2005. K+ inward-rectifying shaker-type channel in barley leaf potentially involved in leaf growth. Abstracts SEB Plant Transport Group Meeting, Glasgow, 7-9 September 2005 .

Microelectrode and 133Cs nuclear magnetic resonance evidence for variable cytosolic and cytoplasmic nitrate pools in maize root tips

A - Papers appearing in refereed journals

Radcliffe, S. A., Miller, A. J. and Ratcliffe, R. G. 2005. Microelectrode and 133Cs nuclear magnetic resonance evidence for variable cytosolic and cytoplasmic nitrate pools in maize root tips. Plant, Cell and Environment. 28 (11), pp. 1379-1387. https://doi.org/10.1111/j.1365-3040.2005.01370.x

Impact of the nematophagous fungus Pochonia chlamydosporia on nematode and microbial populations

A - Papers appearing in refereed journals

Tahseen, Q., Clark, I. M., Atkins, S. D., Hirsch, P. R. and Kerry, B. R. 2005. Impact of the nematophagous fungus Pochonia chlamydosporia on nematode and microbial populations. Communications in Agricultural and Applied Biological Sciences. 70(1), pp. 81-86.

Sensing the physical and nutritional status of the root environment in the field: a review of progress and opportunities

A - Papers appearing in refereed journals

Clark, L. J., Gowing, D. J. G., Lark, R. M., Leeds-Harrison, P. B., Miller, A. J., Wells, D. M., Whalley, W. R. and Whitmore, A. P. 2005. Sensing the physical and nutritional status of the root environment in the field: a review of progress and opportunities. The Journal of Agricultural Science. 143, pp. 347-358. https://doi.org/10.1017/S0021859605005253

Assessing nitrogen-status in two moss species that differ in their nitrogen use

C2 - Non-edited contributions to conferences

Stanley, C. J., Miller, A. J. and Pearson, J. 2005. Assessing nitrogen-status in two moss species that differ in their nitrogen use. Li, C. J. (ed.) Plant Nutrition for Food Security, Human Health and Environmental Protection. Proceedings 15th International Plant Nutrition Colloquium, Beijing, 14-19 September 2005 . Tsinghua University, Beijing. pp. 196-197

Root nitrogen acquisition and assimilation

A - Papers appearing in refereed journals

Miller, A. J. and Cramer, M. D. 2005. Root nitrogen acquisition and assimilation. Plant and Soil. 274 (1-2), pp. 1-36. https://doi.org/10.1007/s11104-004-0965-1

Light-dark changes in cytosolic nitrogen pools depend on nitrate reductase activity in Arabidopsis leaf cells

A - Papers appearing in refereed journals

Cookson, S. J., Williams, L. E. and Miller, A. J. 2005. Light-dark changes in cytosolic nitrogen pools depend on nitrate reductase activity in Arabidopsis leaf cells. Plant Physiology. 138, pp. 1097-1105.

Changes in the microbial community of an arable soil caused by long-term metal contamination

A - Papers appearing in refereed journals

Abaye, D. A., Lawlor, K., Hirsch, P. R. and Brookes, P. C. 2005. Changes in the microbial community of an arable soil caused by long-term metal contamination. European Journal of Soil Science. 56 (1), pp. 93-102. https://doi.org/10.1111/j.1365-2389.2004.00648.x

A two-component high-affinity nitrate uptake system in barley

A - Papers appearing in refereed journals

Tong, Y., Zhou, J-J., Li, Z. and Miller, A. J. 2005. A two-component high-affinity nitrate uptake system in barley. The Plant Journal. 41 (3), pp. 442-450. https://doi.org/10.1111/j.1365-313X.2004.02310.x

The use of real-time PCR and species-specific primers for the identification and monitoring of Paecilomyces lilacinus

A - Papers appearing in refereed journals

Atkins, S. D., Clark, I. M., Pande, S., Hirsch, P. R. and Kerry, B. R. 2005. The use of real-time PCR and species-specific primers for the identification and monitoring of Paecilomyces lilacinus. FEMS Microbiology Ecology. 51 (2), pp. 257-264. https://doi.org/10.1016/j.femsec.2004.09.002

Novel method to investigate microbial community structure in microsites in the rhizosphere

C2 - Non-edited contributions to conferences

Dennis, P. G., Miller, A. J., Valsami-Jones, E., Taylor, R. G. and Hirsch, P. R. 2004. Novel method to investigate microbial community structure in microsites in the rhizosphere. Abstracts International Congress on the Rhizosphere: Perspectives and Challenges - a Tribute to Lorenz Hiltner, Munich, 12-17 September 2004 . pp. 184

Detection of microbial DNA sequences by colony hybridization

B - Book chapters etc edited externally

Hirsch, P. R. 2004. Detection of microbial DNA sequences by colony hybridization. in: Kowalchuk, G. A. (ed.) Molecular microbial ecology manual, 2nd ed. Part 1 Kluwer Academic Publishers, Dordrecht, Netherlands. pp. 345-356

Impact of genetically modified bacterial inoculants on the soil microbial community

C1 - Edited contributions to conferences/learned societies

Hirsch, P. R. and Clark, I. M. 2004. Impact of genetically modified bacterial inoculants on the soil microbial community. GM Crops - Ecological Dimensions. Aspects of Applied Biology 74 . pp. 141-153

Tree leaf biomarkers for atmospheric nitrogen deposition

A - Papers appearing in refereed journals

March, S., Miller, A. J., Zhang, X-H. and Pearson, J. 2004. Tree leaf biomarkers for atmospheric nitrogen deposition. Water, Air, & Soil Pollution: Focus. 4 (6), pp. 241-250. https://doi.org/10.1007/s11267-005-3034-8

Ion-coupled transport of inorganic solutes

B - Book chapters etc edited externally

Hawkesford, M. J. and Miller, A. J. 2004. Ion-coupled transport of inorganic solutes. in: Blatt, M. R. (ed.) Membrane transport in plants Blackwell Publishing Oxford. pp. 105-134

Release of transgenic bacterial inoculants - rhizobia as a case study

A - Papers appearing in refereed journals

Hirsch, P. R. 2004. Release of transgenic bacterial inoculants - rhizobia as a case study. Plant and Soil. 266, pp. 1-10.

Electrophysiological analysis of sensory responses and stylet activity of second stage juveniles of Globodera rostochiensis and plant reactions to nematode invasion

A - Papers appearing in refereed journals

Perry, R. N., Rolfe, R. N., Sheridan, J. P. and Miller, A. J. 2004. Electrophysiological analysis of sensory responses and stylet activity of second stage juveniles of Globodera rostochiensis and plant reactions to nematode invasion. Japanese journal of nematology. 34, pp. 1-10.

Dissecting the tri-trophic interaction between Pochonia chlamydosporia , root-knot nematodes and their plant hosts

C1 - Edited contributions to conferences/learned societies

Morton, C. O., Atkins, S. D., Hirsch, P. R. and Kerry, B. R. 2004. Dissecting the tri-trophic interaction between Pochonia chlamydosporia , root-knot nematodes and their plant hosts.

Biological variation in Verticillium chlamydosporium isolated from different nematode hosts

C1 - Edited contributions to conferences/learned societies

Morton, C. O., Hirsch, P. R., Peberdy, J. and Kerry, B. R. 2004. Biological variation in Verticillium chlamydosporium isolated from different nematode hosts.

Variation in Pochonia chlamydosporia and its potential as a biological control agent for root-knot nematodes

C1 - Edited contributions to conferences/learned societies

Kerry, B. R., Atkins, S. D., Mauchline, T. H., Morton, C. O. and Hirsch, P. R. 2004. Variation in Pochonia chlamydosporia and its potential as a biological control agent for root-knot nematodes.

Investigation of three nematophagous fungi in two potato cyst nematode suppressive soils

C1 - Edited contributions to conferences/learned societies

Atkins, S. D., Sosnowska, D., Evans, V. J., Clark, I. M., Hirsch, P. R. and Kerry, B. R. 2004. Investigation of three nematophagous fungi in two potato cyst nematode suppressive soils.

Infection of plant-parasitic nematodes by nematophagous fungi - a review of the application of molecular biology to understand infection processes and to improve biological control

A - Papers appearing in refereed journals

Morton, C. O., Hirsch, P. R. and Kerry, B. R. 2004. Infection of plant-parasitic nematodes by nematophagous fungi - a review of the application of molecular biology to understand infection processes and to improve biological control. Nematology. 6, pp. 161-170.

Development of a transformation system for the nematophagous fungus Pochonia chlamydosporia

A - Papers appearing in refereed journals

Atkins, S. D., Mauchline, T. H., Kerry, B. R. and Hirsch, P. R. 2004. Development of a transformation system for the nematophagous fungus Pochonia chlamydosporia. Mycological Research. 108 (6), pp. 654-661. https://doi.org/10.1017/S0953756204009906

The biocontrol fungus Pochonia chlamydosporia shows nematode host preference at the infraspecific level

A - Papers appearing in refereed journals

Mauchline, T. H., Kerry, B. R. and Hirsch, P. R. 2004. The biocontrol fungus Pochonia chlamydosporia shows nematode host preference at the infraspecific level. Mycological Research. 108 (2), pp. 161-169. https://doi.org/10.1017/S095375620300889X

Early responses of resistant and susceptible potato roots during invasion by the potato cyst nematode Globodera rostochiensis

A - Papers appearing in refereed journals

Sheridan, J. P., Miller, A. J. and Perry, R. N. 2004. Early responses of resistant and susceptible potato roots during invasion by the potato cyst nematode Globodera rostochiensis. Journal of Experimental Botany. 55 (397), pp. 751-760. https://doi.org/10.1093/jxb/erh063

Isolates of the generalist parasite Pochonia chlamydosporia have specific interactions with their nematode hosts

C2 - Non-edited contributions to conferences

Kerry, B. R., Morton, C. O., Mauchline, T. H. and Hirsch, P. R. 2003. Isolates of the generalist parasite Pochonia chlamydosporia have specific interactions with their nematode hosts. Abstracts British Ecological Society 2003 Annual Symposium on Biological Diversity and Function in Soils, Lancaster, 25-27 March 2003 . pp. 26

Detection and quantification of Plectosphaerella cucumerina , a potential biological control agent of potato cyst nematodes, using real-time PCR

C2 - Non-edited contributions to conferences

Atkins, S. D., Clark, I. M., Sosnowska, D., Hirsch, P. R. and Kerry, B. R. 2003. Detection and quantification of Plectosphaerella cucumerina , a potential biological control agent of potato cyst nematodes, using real-time PCR. Abstracts 11th International Congress on Molecular Plant Microbe Interactions, St Petersburg, 18-27 July 2003 .

Soil sensors for nitrogen availability

C2 - Non-edited contributions to conferences

Miller, A. J., Wells, D. M., Braven, J., Ebdon, L. and Le Goff, T. 2003. Soil sensors for nitrogen availability. Abstracts DEFRA AR09 Workshop, Cranfield, 14 October 2003 .

Novel sensors for measuring soil nitrogen, water availability and strength

C1 - Edited contributions to conferences/learned societies

Miller, A. J., Wells, D. M., Braven, J., Ebdon, L., Le Goff, T., Clark, L. J., Whalley, W. R., Gowing, D. J. G. and Leeds-Harrison, P. B. 2003. Novel sensors for measuring soil nitrogen, water availability and strength. Proceedings BCPC International Congress - Crop Science and Technology, Glasgow, 10-12 November 2003 . pp. 1107-1114

How does Pochonia chlamydosporia limit populations of root-knot nematodes?

C2 - Non-edited contributions to conferences

Kerry, B. R., Atkins, S. D., Gray, P., Hidalgo-Diaz, L., Hirsch, P. R., Mauchline, T. H. and Morton, C. O. 2003. How does Pochonia chlamydosporia limit populations of root-knot nematodes?

Nutrient dynamics in the rhizosphere

C2 - Non-edited contributions to conferences

Hirsch, P. R. and Miller, A. J. 2003. Nutrient dynamics in the rhizosphere.

A case study: the soil ecosystem

C2 - Non-edited contributions to conferences

Hirsch, P. R. 2003. A case study: the soil ecosystem. Abstracts Royal Society Discussion Meeting on GM Crops, Modern Agriculture and the Environment, London, 11 February 2003 .

The influence of the root-knot nematode Meloidogyne incognita , the nematicide aldicarb and the nematophagous fungus Pochonia chlamydosporia on heterotrophic bacteria in soil and the rhizosphere

A - Papers appearing in refereed journals

O'flaherty, S. M., Hirsch, P. R. and Kerry, B. R. 2003. The influence of the root-knot nematode Meloidogyne incognita , the nematicide aldicarb and the nematophagous fungus Pochonia chlamydosporia on heterotrophic bacteria in soil and the rhizosphere. European Journal of Soil Science. 54 (4), pp. 759-766. https://doi.org/10.1046/j.1365-2389.2003.00568.x

Detection and quantification of Plectosphaerella cucumerina , a potential biological control agent of potato cyst nematodes, by using conventional PCR, real-time PCR, selective media, and baiting

A - Papers appearing in refereed journals

Atkins, S. D., Clark, I. M., Sosnowska, D., Hirsch, P. R. and Kerry, B. R. 2003. Detection and quantification of Plectosphaerella cucumerina , a potential biological control agent of potato cyst nematodes, by using conventional PCR, real-time PCR, selective media, and baiting. Applied and Environmental Microbiology. 69, pp. 4788-4793.

Cloning of and genetic variation in protease VCP1 from the nematophagous fungus Pochonia chlamydosporia

A - Papers appearing in refereed journals

Morton, C. O., Hirsch, P. R., Peberdy, J. P. and Kerry, B. R. 2003. Cloning of and genetic variation in protease VCP1 from the nematophagous fungus Pochonia chlamydosporia. Mycological Research. 107 (1), pp. 38-46. https://doi.org/10.1017/S0953756202007050

Single-cell measurements of the contributions of cytosolic Na+ and K+ to salt tolerance

A - Papers appearing in refereed journals

Carden, D. E., Walker, D. J., Flowers, T. J. and Miller, A. J. 2003. Single-cell measurements of the contributions of cytosolic Na+ and K+ to salt tolerance. Plant Physiology. 131, pp. 676-683.

Potassium activities in cell compartments of salt-grown barley leaves

A - Papers appearing in refereed journals

Cuin, T. A., Miller, A. J., Laurie, S. A. and Leigh, R. A. 2003. Potassium activities in cell compartments of salt-grown barley leaves. Journal of Experimental Botany. 54, pp. 657-661.

Development of a new management strategy for the control of root-knot nematodes (Meloidogyne spp) in organic vegetable production

A - Papers appearing in refereed journals

Atkins, S. D., Hidalgo-Diaz, L., Kalisz, H., Mauchline, T. H., Hirsch, P. R. and Kerry, B. R. 2003. Development of a new management strategy for the control of root-knot nematodes (Meloidogyne spp) in organic vegetable production. Pest Management Science. 59 (2), pp. 183-189. https://doi.org/10.1002/ps.603

PCR-based DNA fingerprinting indicates host-related genetic variation in the nematophagous fungus Pochonia chlamydosporia

A - Papers appearing in refereed journals

Morton, C. O., Mauchline, T. H., Kerry, B. R. and Hirsch, P. R. 2003. PCR-based DNA fingerprinting indicates host-related genetic variation in the nematophagous fungus Pochonia chlamydosporia. Mycological Research. 107, pp. 198-205.

Electrical activity in potato roots induced by Globodera rostochiensis

C2 - Non-edited contributions to conferences

Sheridan, J. P., Miller, A. J. and Perry, R. N. 2003. Electrical activity in potato roots induced by Globodera rostochiensis. Abstracts 55th International Symposium on Crop Protection, Ghent, 6 May 2003 .

Variance in Pochonia chlamydosporia and its importance in the regulation of nematode populations in the rhizosphere

C2 - Non-edited contributions to conferences

Kerry, B. R., Morton, C. O., Mauchline, T. H. and Hirsch, P. R. 2002. Variance in Pochonia chlamydosporia and its importance in the regulation of nematode populations in the rhizosphere. Abstracts Symposium on the Role of Genetics and Evolution in Biological Control, Montpellier, 14-16 October 2002 .

Isolates of the generalist parasite Pochonia chlamydosporia have specific interactions with their nematode hosts

C2 - Non-edited contributions to conferences

Kerry, B. R., Morton, C. O., Mauchline, T. H. and Hirsch, P. R. 2002. Isolates of the generalist parasite Pochonia chlamydosporia have specific interactions with their nematode hosts. Abstracts 4th International Congress of Nematology, Tenerife, 8-13 June 2002 .

Impact of GM bacterial inoculants on the soil microbial community

C2 - Non-edited contributions to conferences

Hirsch, P. R. and Clark, I. M. 2002. Impact of GM bacterial inoculants on the soil microbial community. Abstracts International Conference on GMOs - Ecological Dimensions, Reading, 9-11 September 2002 .

Release of transgenic bacterial inoculants in Europe

C2 - Non-edited contributions to conferences

Hirsch, P. R. 2002. Release of transgenic bacterial inoculants in Europe. Abstracts Symposium on the Impact of GMOs: Soil Microbiology and Nutrient Dynamics, Vienna, 3-6 November 2002 .

Fate of GM rhizobial inoculants: lessons from Europe and elsewhere

C1 - Edited contributions to conferences/learned societies

Hirsch, P. R. 2002. Fate of GM rhizobial inoculants: lessons from Europe and elsewhere. Proceedings 7th International Symposium on the Biosafety of GMOs, Beijing, 10-17 October 2002 . pp. 243-250

Genetic and functional diversity in the rhizosphere of oilseed rape, wheat and potatoes

C2 - Non-edited contributions to conferences

Gilliam, L. M., Clark, I. M. and Hirsch, P. R. 2002. Genetic and functional diversity in the rhizosphere of oilseed rape, wheat and potatoes. Abstracts BAGECO-7: 7th Symposium on Bacterial Genetics and Ecology, Bergen, 15-19 June 2002 . pp. 86

Interactions in the root environment - an integrated approach. The Rothamsted Millennium Conference

E - Books edited/written by staff member, alone/with others

Powlson, D. S., Bateman, G. L., Davies, K. G., Gaunt, J. L. and Hirsch, P. R. Barlow, P. W. (ed.) 2002. Interactions in the root environment - an integrated approach. The Rothamsted Millennium Conference. Kluwer Academic Publishers, Dordrecht, Netherlands.

Changes in the population structure of beta-group autotrophic ammonia oxidising bacteria in arable soils in response to agricultural practice

A - Papers appearing in refereed journals

Mendum, T. A. and Hirsch, P. R. 2002. Changes in the population structure of beta-group autotrophic ammonia oxidising bacteria in arable soils in response to agricultural practice. Soil Biology and Biochemistry. 34, pp. 1479-1485.

The influence of the symbiotic plasmid pRL1J1 on the distribution of GM rhizobia in soil and crop rhizospheres, and implications for gene flow

A - Papers appearing in refereed journals

Clark, I. M., Mendum, T. A. and Hirsch, P. R. 2002. The influence of the symbiotic plasmid pRL1J1 on the distribution of GM rhizobia in soil and crop rhizospheres, and implications for gene flow. Antonie van Leeuwenhoek. 81, pp. 607-616.

The role of the rhizobium symbiotic plasmid in survival in the soil and the rhizosphere

C2 - Non-edited contributions to conferences

Clark, I. M. and Hirsch, P. R. 2002. The role of the rhizobium symbiotic plasmid in survival in the soil and the rhizosphere. Abstracts BAGECO-7: 7th Symposium on Bacterial Genetics and Ecology, Bergen, 15-19 June 2002 .

The fate of genetically modified rhizobial inoculants in the field

C2 - Non-edited contributions to conferences

Hirsch, P. R. and Clark, I. M. 2002. The fate of genetically modified rhizobial inoculants in the field. Abstracts 8th New Phytologist Symposium, Helsinki, 10-14 June 2002 .

Quantification in soil and the rhizosphere of the nematophagous fungus Verticillium chlamydosporium by competitive PCR and comparison with selective plating

A - Papers appearing in refereed journals

Mauchline, T. H., Kerry, B. R. and Hirsch, P. R. 2002. Quantification in soil and the rhizosphere of the nematophagous fungus Verticillium chlamydosporium by competitive PCR and comparison with selective plating. Applied and Environmental Microbiology. 68, pp. 1846-1853.

Failure of the Mycobacterium bovis BCG vaccine: some species of environmental mycobacteria block multiplication of BCG and induction of protective immunity to tuberculosis

A - Papers appearing in refereed journals

Brandt, L., Feino Cunha, J., Weinreich O. A., Chilima, B., Hirsch, P. R., Appelberg, R. and Andersen, P. 2002. Failure of the Mycobacterium bovis BCG vaccine: some species of environmental mycobacteria block multiplication of BCG and induction of protective immunity to tuberculosis. Infection And Immunity. 70, pp. 672-678. https://doi.org/10.1128/iai.70.2.672-678.2002

Foreword to Proceedings of the Rothamsted Millennium Conference on Interactions in the Root Environment

G - Articles in popular magazines and other technical publications

Powlson, D. S., Bateman, G. L., Davies, K. G., Gaunt, J. L., Hirsch, P. R. and Barlow, P. W. 2001. Foreword to Proceedings of the Rothamsted Millennium Conference on Interactions in the Root Environment. Plant and Soil. 232, pp. 1-2.

Ammonium concentrations inside root cells measured using an improved ion-selective microelectrode

C2 - Non-edited contributions to conferences

Wells, D. M., Pearson, J. and Miller, A. J. 2001. Ammonium concentrations inside root cells measured using an improved ion-selective microelectrode. Abstracts 6th International Symposium on Inorganic Nitrogen Assimilation, Reims, 8-12 July 2001 . pp. 3-50

Root cell heterogeneity in response to changes in nitrogen supply

C2 - Non-edited contributions to conferences

Smith, S. J. and Miller, A. J. 2001. Root cell heterogeneity in response to changes in nitrogen supply. Abstracts 6th International Symposium on Inorganic Nitrogen Assimilation, Reims, 8-12 July 2001 .

Book review: Vacuolar compartments by D. G. Robinson and J. C. Roger

G - Articles in popular magazines and other technical publications

Miller, A. J. 2001. Book review: Vacuolar compartments by D. G. Robinson and J. C. Roger. Annals of Botany. 88, pp. 1219-1220.

Comparison of the properties of two nitrate transporters from Brassica napus

C2 - Non-edited contributions to conferences

Cryer, N., Ingemarsson, B., Pyke, K. and Miller, A. J. 2001. Comparison of the properties of two nitrate transporters from Brassica napus. Abstracts 6th International Symposium on Inorganic Nitrogen Assimilation, Reims, 8-12 July 2001 . pp. 2.11

How dissolved inorganic carbon influences N uptake

C2 - Non-edited contributions to conferences

Cramer, M. and Miller, A. J. 2001. How dissolved inorganic carbon influences N uptake. Abstracts 6th International Symposium on Inorganic Nitrogen Assimilation, Reims, 8-12 July 2001 . pp. 2.1

Light/dark changes in cytosolic nitrate activities in leaf cells: the role of nitrate reductase

C2 - Non-edited contributions to conferences

Cookson, S. J. and Miller, A. J. 2001. Light/dark changes in cytosolic nitrate activities in leaf cells: the role of nitrate reductase. Abstracts 6th International Symposium on Inorganic Nitrogen Assimilation, Reims, 8-12 July 2001 . pp. 3-8

The role of soil microorganisms in soil organic matter conservation in the tropics

A - Papers appearing in refereed journals

Powlson, D. S., Hirsch, P. R. and Brookes, P. C. 2001. The role of soil microorganisms in soil organic matter conservation in the tropics. Nutrient Cycling in Agroecosystems. 61 (1), pp. 41-51. https://doi.org/10.1023/A:1013338028454

Transgenic releases, analysis of gene flow and fluctuations in indigenous bacterial communities

D1 - Technical reports: non-confidential

Hirsch, P. R. 2001. Transgenic releases, analysis of gene flow and fluctuations in indigenous bacterial communities. European Commission, Luxembourg.

Acquisition of genes from indigenous bacteria by inoculant strains at long-term release sites

D1 - Technical reports: non-confidential

Hirsch, P. R. 2001. Acquisition of genes from indigenous bacteria by inoculant strains at long-term release sites. European Commission, Luxembourg.

Assessing the risks involved in the release of genetically manipulated microorganisms

D1 - Technical reports: non-confidential

Amarger, N., Hirsch, P. R. and Klingmuller, W. 2001. Assessing the risks involved in the release of genetically manipulated microorganisms. European Commission, Luxembourg.

Analysis of electrical activity in potato roots in response to the potato cyst nematode Globodera rostochiensis

C2 - Non-edited contributions to conferences

Sheridan, J. P., Miller, A. J. and Perry, R. N. 2001. Analysis of electrical activity in potato roots in response to the potato cyst nematode Globodera rostochiensis. Abstracts APS-SON-MSA Joint Meeting, Salt Lake City, 25-29 August 2001 .

Measurement of the impact of Verticillium chlamydosporium on the dynamics of sedentary nematodes in the rhizosphere

C1 - Edited contributions to conferences/learned societies

Kerry, B. R., Atkins, S. D., Mauchline, T. H. and Hirsch, P. R. 2001. Measurement of the impact of Verticillium chlamydosporium on the dynamics of sedentary nematodes in the rhizosphere.

Understanding and exploiting interactions between nitrogen-fixing bacteria and plants

B - Book chapters etc edited externally

Hirsch, P. R. and Micanovic, D. 2001. Understanding and exploiting interactions between nitrogen-fixing bacteria and plants. in: Quarrie, S. A., Stojanovic, S., Knezevic, D., Atanassov, A. and Janjic, V. (ed.) Genetics and breeding of small grains ARI-Serbia, Belgrade. pp. 335-347

Soil microbial fatty acids as indicators of heavy metal pollution and soil quality

C2 - Non-edited contributions to conferences

Abaye, D., Lawlor, K., Hirsch, P. R. and Brookes, P. C. 2001. Soil microbial fatty acids as indicators of heavy metal pollution and soil quality. Abstracts 9th International Symposium on Microbial Ecology (ISME 9): Interactions in the Microbial World, Amsterdam, 26-31 August 2001 . pp. 204

An improved Na+-selective microelectrode for intracellular measurements in plant cells

A - Papers appearing in refereed journals

Carden, D. E., Diamond, D. and Miller, A. J. 2001. An improved Na+-selective microelectrode for intracellular measurements in plant cells. Journal of Experimental Botany. 52, pp. 1353-1359.

Characterization of two novel Rhizobium leguminosarum bacteriophages from a field release site of genetically-modified rhizobia

A - Papers appearing in refereed journals

Mendum, T. A., Clark, I. M. and Hirsch, P. R. 2001. Characterization of two novel Rhizobium leguminosarum bacteriophages from a field release site of genetically-modified rhizobia. Antonie van Leeuwenhoek. 79 (2), pp. 189-197. https://doi.org/10.1023/a:1010238412538

Molecular approaches to studies of the interactions of Verticillium chlamydosporium in the rhizosphere of root-knot nematode infected plants

C2 - Non-edited contributions to conferences

Kerry, B. R., O'flaherty, S., Mauchline, T. H., Morton, C. O. and Hirsch, P. R. 2001. Molecular approaches to studies of the interactions of Verticillium chlamydosporium in the rhizosphere of root-knot nematode infected plants. Abstracts 4th International Scientific Seminar of Plant Health/33rd Annual Meeting of the Organization of Nematologists of Tropical America (ONTA), Varadera, Cuba, 11-15 June 2001 . pp. 12-13

Methods for studying the nematophagous fungus Verticillium chlamydosporium in the root environment

A - Papers appearing in refereed journals

Hirsch, P. R., Atkins, S. D., Mauchline, T. H., Morton, C. O., Davies, K. G. and Kerry, B. R. 2001. Methods for studying the nematophagous fungus Verticillium chlamydosporium in the root environment. Plant and Soil. 232, pp. 21-30.

Microbial diversity in heavy metal-contaminated soils - microbial indicators of soil quality

C2 - Non-edited contributions to conferences

Abaye, D., Lawlor, K., Hirsch, P. R. and Brookes, P. C. 2001. Microbial diversity in heavy metal-contaminated soils - microbial indicators of soil quality. Proceedings Chiba University International Symposium on Microbial Diversity and Environmental Remediation in Biosphere, Nishi-Chiba, Japan, 6 July 2001 .

Comparison of phenotypic, functional and genetic diversity of bacterial communities in soils

B - Book chapters etc edited externally

O'flaherty, S., McGrath, S. P. and Hirsch, P. R. 2001. Comparison of phenotypic, functional and genetic diversity of bacterial communities in soils. in: Ball, B. C., Watson, C. A., Campbell, C. D. and Rees, R. M. (ed.) Sustainable management of soil organic matter CABI International, Wallingford, Oxon (CABI). pp. 370-376

Transporters responsible for the uptake and partitioning of nitrogenous solutes

A - Papers appearing in refereed journals

Williams, L. E. and Miller, A. J. 2001. Transporters responsible for the uptake and partitioning of nitrogenous solutes. Annual Review of Plant Physiology and Plant Molecular Biology. 52, pp. 659-688.

The use of microelectrodes to investigate compartmentation and the transport of metabolized inorganic ions in plants

A - Papers appearing in refereed journals

Miller, A. J., Cookson, S. J., Smith, S. J. and Wells, D. M. 2001. The use of microelectrodes to investigate compartmentation and the transport of metabolized inorganic ions in plants. Journal of Experimental Botany. 52, pp. 541-549.

Molecular methods to study the rhizosphere interactions of Verticillium chlamydosporium

C2 - Non-edited contributions to conferences

Kerry, B. R., Mauchline, T. H., Atkins, S. D., Davies, K. G. and Hirsch, P. R. 2000. Molecular methods to study the rhizosphere interactions of Verticillium chlamydosporium. Abstracts 39th Annual Meeting of the Society of Nematologists, Quebec, 26-28 June 2000 .

A high affinity fungal nitrate carrier with two transport mechanisms

A - Papers appearing in refereed journals

Zhou, J-J., Trueman, L. J., Boorer, K. J., Theodoulou, F. L., Forde, B. G. and Miller, A. J. 2000. A high affinity fungal nitrate carrier with two transport mechanisms. Journal of Biological Chemistry. 275, pp. 39894-39899.

Improving fertiliser use efficiency in agro-ecosystems and nutrient efficiency in plants

C1 - Edited contributions to conferences/learned societies

Von Wiren, N., Andre, B., Harling, H., Gojon, A., Patriarca, E., Merrick, M., Miller, A. J., Reiss, B. and Frommer, W. B. 2000. Improving fertiliser use efficiency in agro-ecosystems and nutrient efficiency in plants. Devries, G. E. and Metzlaff, K. (ed.) Phytosfere '99: Highlights in European Plant Biotechnology Research and Technology Transfer. Proceedings 2nd European Conference on Plant Biotechnology, Rome, 7-9 June 1999 . Elsevier, Amsterdam. pp. 225-233

Characterisation of nitrate transport using nitrate-selective microelectrodes and heterologous expression in Xenopus oocytes

B - Book chapters etc edited externally

Smith, S. J., Zhou, J-J. and Miller, A. J. 2000. Characterisation of nitrate transport using nitrate-selective microelectrodes and heterologous expression in Xenopus oocytes. in: Canovas, F. M. and Florencio, F. J. (ed.) Avances en el metabolismo del nitrogeno: bioquimica, fisiologia y biologia Universidad de Malaga, Malaga. pp. 345-350

Determination of mosquito bloodmeal pH in situ by ion-selective microelectrode measurement: implications for the regulation of malarial gametogenesis

A - Papers appearing in refereed journals

Billker, O., Miller, A. J. and Sinden, R. E. 2000. Determination of mosquito bloodmeal pH in situ by ion-selective microelectrode measurement: implications for the regulation of malarial gametogenesis. Parasitology. 120 (6), pp. 547-551. https://doi.org/10.1017/S0031182099005946

The diversity, activity and size of ammonia oxidising populations

C2 - Non-edited contributions to conferences

Mendum, T. A. and Hirsch, P. R. 2000. The diversity, activity and size of ammonia oxidising populations. Abstracts British Society of Soil Science Conference Eurosoil 2000, Reading, 4-6 September 2000 .

Inference of symbiotic plasmid involvement in the population dynamics of two GM rhizobia under different agricultural practices

C2 - Non-edited contributions to conferences

Clark, I. M., Mendum, T. A. and Hirsch, P. R. 2000. Inference of symbiotic plasmid involvement in the population dynamics of two GM rhizobia under different agricultural practices. Abstracts 2nd Symposium of the EU Concerted Action on Mobile Genetic Elements' Contribution to Bacterial Adaptability and Diversity (MECBAD), Prague, 17-19 September 2000 . pp. 27-28

Soil microbial fatty acids as indicators of soil quality

C2 - Non-edited contributions to conferences

Abaye, D., Lawlor, K., Hirsch, P. R. and Brookes, P. C. 2000. Soil microbial fatty acids as indicators of soil quality. Abstracts British Society of Soil Science Conference Eurosoil 2000, Reading, 4-6 September 2000 .

Comparison of the transport properties of three plant sucrose carriers expressed in Xenopus oocytes

A - Papers appearing in refereed journals

Zhou, J-J. and Miller, A. J. 2000. Comparison of the transport properties of three plant sucrose carriers expressed in Xenopus oocytes. Australian journal of plant physiology. 27, pp. 725-732.

A high affinity nitrate transport system from Chlamydomonas requires two gene products

A - Papers appearing in refereed journals

Zhou, J-J., Fernandez, E., Galvan, A. and Miller, A. J. 2000. A high affinity nitrate transport system from Chlamydomonas requires two gene products. FEBS Letters. 466 (1), pp. 225-227. https://doi.org/10.1016/S0014-5793(00)01955-4

Intracellular measurement of ammonium in Chara corallina using ion-selective microelectrodes

A - Papers appearing in refereed journals

Wells, D. M. and Miller, A. J. 2000. Intracellular measurement of ammonium in Chara corallina using ion-selective microelectrodes. Plant and Soil. 221, pp. 103-106.

Xenopus oocytes as an expression system for plant transporters

A - Papers appearing in refereed journals

Miller, A. J. and Zhou, J-J. 2000. Xenopus oocytes as an expression system for plant transporters. Biochimica et Biophysica Acta (BBA) - Biomembranes. 1465 (1-2), pp. 343-358. https://doi.org/10.1016/S0005-2736(00)00148-6

Development of a transformation system for the root-knot nematode biological control fungus Verticillium chlamydosporium

B - Book chapters etc edited externally

Atkins, S. D., Peberdy, J., Kerry, B. R. and Hirsch, P. R. 2000. Development of a transformation system for the root-knot nematode biological control fungus Verticillium chlamydosporium. in: de Wit, P. J. G. M., Stiekema, W. J. and Bisseling, T. (ed.) Biology of plant-microbe interactions. Vol. 2 International Society for Molecular Plant-Microbe Interactions, St Paul. pp. 336-341

Site-directed mutagenesis of sucrose carriers confirms the role of phosphorylation in regulating transport activity

C2 - Non-edited contributions to conferences

Zhou, J-J. and Miller, A. J. 2000. Site-directed mutagenesis of sucrose carriers confirms the role of phosphorylation in regulating transport activity. Abstracts Society for Experimental Biology Plant Transport Group Meeting, Hatfield, 6-9 September 2000 .

Development of a novel ammonium-selective microelectrode

C2 - Non-edited contributions to conferences

Wells, D. M., Pearson, J. and Miller, A. J. 2000. Development of a novel ammonium-selective microelectrode. Abstracts Society for Experimental Biology Plant Transport Group Meeting, Hatfield, 6-9 September 2000 .

Electrophysiological techniques to study intact, aerial Arabidopsis thaliana leaf cells

C2 - Non-edited contributions to conferences

Cookson, S. J. and Miller, A. J. 2000. Electrophysiological techniques to study intact, aerial Arabidopsis thaliana leaf cells. Abstracts Society for Experimental Biology Plant Transport Group Meeting, Hatfield, 6-9 September 2000 .

Comparison of methods to investigate microbial populations in soils under different agricultural management

A - Papers appearing in refereed journals

Lawlor, K., Knight, B. P., Barbosa-Jefferson, V. L., Lane, P. W., Lilley, A. K., Paton, G. I., McGrath, S. P., O'flaherty, S. and Hirsch, P. R. 2000. Comparison of methods to investigate microbial populations in soils under different agricultural management. FEMS Microbiology Ecology. 33, pp. 129-137. https://doi.org/10.1111/j.1574-6941.2000.tb00735.x

Ecology of plasmid transfer and spread

B - Book chapters etc edited externally

Van Elsas, J. D., Fry, J., Hirsch, P. R. and Molin, S. 2000. Ecology of plasmid transfer and spread. in: Thomas, C. M. (ed.) The horizontal gene pool: bacterial plasmids and gene spread Harwood Academic Publishers, Amsterdam. pp. 175-206

The PCR amplification of non-tuberculous mycobacterial 16S rRNA sequences from soil

A - Papers appearing in refereed journals

Mendum, T. A., Chilima, B. Z. and Hirsch, P. R. 2000. The PCR amplification of non-tuberculous mycobacterial 16S rRNA sequences from soil. FEMS Microbiology Letters. 185, pp. 189-192. https://doi.org/10.1111/j.1574-6968.2000.tb09060.x

Detection of the nematophagous fungus Verticillium chlamydosporium in nematode-infested plant roots using PCR

A - Papers appearing in refereed journals

Hirsch, P. R., Mauchline, T. H., Mendum, T. A. and Kerry, B. R. 2000. Detection of the nematophagous fungus Verticillium chlamydosporium in nematode-infested plant roots using PCR. Mycological Research. 104 (4), pp. 435-439. https://doi.org/10.1017/S0953756299001483

Patterns of potassium compartmentation in plant cells as revealed by microelectrodes and microsampling

C1 - Edited contributions to conferences/learned societies

Leigh, R. A., Walker, D. J., Fricke, W., Tomos, A. D. and Miller, A. J. 1999. Patterns of potassium compartmentation in plant cells as revealed by microelectrodes and microsampling. Oosterhuis, D. M. and Berkowitz, G. A. (ed.) Frontiers in Potassium Nutrition: New Perspectives on the Effects of Potassium on Physiology of Plants. Proceedings of a Symposium sponsored by the Potash and Phosphate Institute and the Crop Science Society of America . Potash & Phosphate Institute/Potash & Phosphate Institute of Canada. pp. 63-70

The field release and monitoring of GUS-marked rhizobial strain CT0370

B - Book chapters etc edited externally

Hirsch, P. R., Mendum, T. A., Puhler, A. and Selbitschka, W. 1999. The field release and monitoring of GUS-marked rhizobial strain CT0370. in: Bailey, M. J., Jansson, J. K. and Vanelsas, J. D. (ed.) Tracking genetically-engineered microorganisms Landes Bioscience, Georgetown. pp. 145-151

Compartmentation of potassium in salt stressed barley leaves

C2 - Non-edited contributions to conferences

Cuin, T. A., Miller, A. J., Laurie, S. A. and Leigh, R. A. 1999. Compartmentation of potassium in salt stressed barley leaves. Abstracts Society for Experimental Biology Plant Transport Group Meeting, Southampton, 8-10 September 1999 .

Measurement of ammonium in plant cells using ion-selective microelectrodes

C2 - Non-edited contributions to conferences

Wells, D. M., Pearson, J. and Miller, A. J. 1999. Measurement of ammonium in plant cells using ion-selective microelectrodes. Abstracts Society for Experimental Biology Plant Transport Group Meeting, Southampton, 8-10 September 1999 .

Nitrate signalling in plant cells

C2 - Non-edited contributions to conferences

Cookson, S. J., Williams, L. E. and Miller, A. J. 1999. Nitrate signalling in plant cells. Abstracts Society for Experimental Biology Plant Transport Group Meeting, Southampton, 8-10 September 1999 .

The cloning and characterisation of nitrate influx and efflux systems

C2 - Non-edited contributions to conferences

Cryer, N. C., Pyke, K., Forde, B. G. and Miller, A. J. 1999. The cloning and characterisation of nitrate influx and efflux systems. Abstracts Society for Experimental Biology Plant Transport Group Meeting, Southampton, 8-10 September 1999 .

The regulation and kinetic characterization of plant sucrose carriers

C2 - Non-edited contributions to conferences

Zhou, J-J. and Miller, A. J. 1999. The regulation and kinetic characterization of plant sucrose carriers. Abstracts Society for Experimental Biology Plant Transport Group Meeting, Southampton, 8-10 September 1999 .

Do changes in land management influence soil microbial function?

C2 - Non-edited contributions to conferences

O'flaherty, S. M., Murphy, D. V., Goulding, K. W. T. and Hirsch, P. R. 1999. Do changes in land management influence soil microbial function? Proceedings British Society of Soil Science Meeting on Sustainable Management of Soil Organic Matter, Edinburgh, 15-17 September 1999 .

Microbial diversity - an indication of soil quality?

C2 - Non-edited contributions to conferences

O'flaherty, S. M., McGrath, S. P. and Hirsch, P. R. 1999. Microbial diversity - an indication of soil quality? Proceedings British Society of Soil Science Meeting on Sustainable Management of Soil Organic Matter, Edinburgh, 15-17 September 1999 .

Use of molecular and isotopic techniques to monitor the response of autotrophic ammonia-oxidizing populations of the beta subdivision of the class Proteobacteria in arable soils to nitrogen fertilizer

A - Papers appearing in refereed journals

Mendum, T. A., Sockett, R. E. and Hirsch, P. R. 1999. Use of molecular and isotopic techniques to monitor the response of autotrophic ammonia-oxidizing populations of the beta subdivision of the class Proteobacteria in arable soils to nitrogen fertilizer. Applied and Environmental Microbiology. 65 (9), pp. 4155-4162. https://doi.org/10.1128/AEM.65.9.4155-4162.1999

Investigating genetic and functional shifts in metal contaminated soils

C2 - Non-edited contributions to conferences

O'flaherty, S. M. and Hirsch, P. R. 1999. Investigating genetic and functional shifts in metal contaminated soils. Abstracts BAGECO-6: 6th Symposium on Bacterial Genetics and Ecology, Florence, 20-24 June 1999 . pp. 61

Microbial diversity - an indication of soil quality?

C2 - Non-edited contributions to conferences

O'flaherty, S. M., McGrath, S. P. and Hirsch, P. R. 1999. Microbial diversity - an indication of soil quality? Abstracts Society for General Microbiology 143rd Meeting, Edinburgh, 12-16 April 1999 . pp. 48

Interactions between rhizobia and bacteriophages isolated from soil

C2 - Non-edited contributions to conferences

Mendum, T. A., Clark, I. M. and Hirsch, P. R. 1999. Interactions between rhizobia and bacteriophages isolated from soil. Abstracts BAGECO-6: 6th Symposium on Bacterial Genetics and Ecology, Florence, 20-24 June 1999 . pp. 17

Competitive PCR to monitor ammonia oxidisers in a 150 year old field experiment

C2 - Non-edited contributions to conferences

Mendum, T. A., Sockett, R. E. and Hirsch, P. R. 1999. Competitive PCR to monitor ammonia oxidisers in a 150 year old field experiment. Abstracts Society for General Microbiology 143rd Meeting, Edinburgh, 12-16 April 1999 . pp. 44

The genetic implications of bacteriophages in Rhizobium leguminosarum

C2 - Non-edited contributions to conferences

Mendum, T. A., Clark, I. M. and Hirsch, P. R. 1999. The genetic implications of bacteriophages in Rhizobium leguminosarum. Abstracts 1st Symposium of the EU-concerted Action on Mobile Elements Contribution to Bacterial Adaptability (MECBAD), Le Mont Ste Odile, 23-27 April 1999 . pp. 23

Development of a transformation system for the nematode biological control fungus Verticillium chlamydosporium

C2 - Non-edited contributions to conferences

Atkins, S. D., Peberdy, J., Kerry, B. R. and Hirsch, P. R. 1999. Development of a transformation system for the nematode biological control fungus Verticillium chlamydosporium. Abstracts 9th International Congress on Molecular Plant-Microbe Interactions, Amsterdam, 25-30 July 1999 . pp. 186

Nitrate interference with potassium-selective microelectrodes

A - Papers appearing in refereed journals

Cuin, T. A., Miller, A. J., Laurie, S. A. and Leigh, R. A. 1999. Nitrate interference with potassium-selective microelectrodes. Journal of Experimental Botany. 50, pp. 1709-1712.

Gene transfer in bacteria from soils contaminated with heavy metals

A - Papers appearing in refereed journals

Lawlor, K., Chaudri, A. M., McGrath, S. P. and Hirsch, P. R. 1999. Gene transfer in bacteria from soils contaminated with heavy metals. Letters in Applied Microbiology. 28 (4), pp. 317-320. https://doi.org/10.1046/j.1365-2672.1999.00520.x

Immunological quantification in soil of Pasteuria penetrans , an unculturable bacteria, hyperparasite of plant-parasitic nematodes

C2 - Non-edited contributions to conferences

Fould, S., Davies, K. G., Hirsch, P. R., Dieng, L., Dabire, K. R., Diop, M. T., Normand, P. and Mateille, T. 1998. Immunological quantification in soil of Pasteuria penetrans , an unculturable bacteria, hyperparasite of plant-parasitic nematodes.

The role of cytosolic potassium and pH in the growth of barley roots

A - Papers appearing in refereed journals

Walker, D. J., Black, C. R. and Miller, A. J. 1998. The role of cytosolic potassium and pH in the growth of barley roots. Plant Physiology. 118, pp. 957-964.

Microbial diversity in agricultural soil

C2 - Non-edited contributions to conferences

O'flaherty, S., Lawlor, K., McGrath, S. P., Kerry, B. R. and Hirsch, P. R. 1998. Microbial diversity in agricultural soil. Abstracts British Society for Soil Science Down to Earth Conference, University of Reading, 31 March-2 April 1998 .

Bacterial diversity in metal contaminated soils

C2 - Non-edited contributions to conferences

Lawlor, K., Barbosa-Jefferson, V., McGrath, S. P., O'flaherty, S. and Hirsch, P. R. 1998. Bacterial diversity in metal contaminated soils. Abstracts British Society for Soil Science Down to Earth Conference, University of Reading, 31 March-2 April 1998 .

Characterization of three different higher plant sucrose transporters expressed in Xenopus oocytes

C2 - Non-edited contributions to conferences

Zhou, J-J. and Miller, A. J. 1998. Characterization of three different higher plant sucrose transporters expressed in Xenopus oocytes. Abstracts 11th International Workshop on Plant Membrane Biology, Cambridge, 9-14 August 1998 . pp. 254

Measurement of intracellular ammonium activity using ion-selective microelectrodes

C2 - Non-edited contributions to conferences

Wells, D. M. and Miller, A. J. 1998. Measurement of intracellular ammonium activity using ion-selective microelectrodes. Abstracts 11th International Workshop on Plant Membrane Biology, Cambridge, 9-14 August 1998 . pp. 218

Dynamic measurements of cytosolic nitrate indicate a role in induction

C2 - Non-edited contributions to conferences

Smith, S. J., Ivashikina, N. and Miller, A. J. 1998. Dynamic measurements of cytosolic nitrate indicate a role in induction. Abstracts 11th International Workshop on Plant Membrane Biology, Cambridge, 9-14 August 1998 . pp. 213

The detection of Gram negative bacterial mRNA from soil by RT-PCR

A - Papers appearing in refereed journals

Mendum, T. A., Sockett, R. E. and Hirsch, P. R. 1998. The detection of Gram negative bacterial mRNA from soil by RT-PCR. FEMS Microbiology Letters. 164, pp. 369-373. https://doi.org/10.1111/j.1574-6968.1998.tb13111.x

The effect of external pH on nitrate uptake and compartmentation in maize roots

C2 - Non-edited contributions to conferences

Ivashikina, N. and Miller, A. J. 1998. The effect of external pH on nitrate uptake and compartmentation in maize roots. Abstracts 5th International Symposium on Inorganic Nitrogen Assimilation, Luso, 1998 .

Monitoring genetically modified rhizobia in field soils using the polymerase chain reaction

A - Papers appearing in refereed journals

Cullen, D. W., Nicholson, P. S., Mendum, T. A. and Hirsch, P. R. 1998. Monitoring genetically modified rhizobia in field soils using the polymerase chain reaction. Journal of Applied Microbiology. 84 (6), pp. 1025-1034. https://doi.org/10.1046/j.1365-2672.1998.00443.x

Simple and rapid method for direct extraction of microbial DNA from soil for PCR

A - Papers appearing in refereed journals

Cullen, D. W. and Hirsch, P. R. 1998. Simple and rapid method for direct extraction of microbial DNA from soil for PCR. Soil Biology and Biochemistry. 30 (8/9), pp. 983-993. https://doi.org/10.1016/S0038-0717(98)00001-7

Root cell cytosolic ion differences between barley cultivars may contribute to salt tolerance

C2 - Non-edited contributions to conferences

Carden, D. E., Walker, D. J., Flowers, T. J. and Miller, A. J. 1998. Root cell cytosolic ion differences between barley cultivars may contribute to salt tolerance. Abstracts 11th International Workshop on Plant Membrane Biology, Cambridge, 9-14 August 1998 . pp. 345

Cloning and functional characterization of a Brassica napus transporter that is able to transport nitrate and histidine

A - Papers appearing in refereed journals

Zhou, J-J., Theodoulou, F. L., Muldin, I., Ingemarsson, B. and Miller, A. J. 1998. Cloning and functional characterization of a Brassica napus transporter that is able to transport nitrate and histidine. Journal of Biological Chemistry. 273 (20), pp. 12017-12023. https://doi.org/10.1074/jbc.273.20.12017

Remobilisation of vacuolar stored nitrate in barley root cells

A - Papers appearing in refereed journals

Van Der Leij, M., Smith, S. J. and Miller, A. J. 1998. Remobilisation of vacuolar stored nitrate in barley root cells. Planta. 205, pp. 64-72.

The effects of pesticides on the diversity of culturable soil bacteria

A - Papers appearing in refereed journals

Nicholson, P. S. and Hirsch, P. R. 1998. The effects of pesticides on the diversity of culturable soil bacteria. Journal of Applied Microbiology. 84 (4), pp. 551-558. https://doi.org/10.1046/j.1365-2672.1998.00381.x

Plant genotype and micronutrient status influence colonization of wheat roots by soil bacteria

A - Papers appearing in refereed journals

Rengel, Z., Ross, G. and Hirsch, P. R. 1998. Plant genotype and micronutrient status influence colonization of wheat roots by soil bacteria. Journal of Plant Nutrition. 21 (1), pp. 99-113. https://doi.org/10.1080/01904169809365386

Acquisition of genes from indigenous bacteria by inoculant strains at long-term release sites

D1 - Technical reports: non-confidential

Hirsch, P. R. 1997. Acquisition of genes from indigenous bacteria by inoculant strains at long-term release sites. European Commission, Luxembourg.

Occurrence of flavonoids and nucleosides in agricultural soils

A - Papers appearing in refereed journals

Phillips, D. A., Joseph, C. M. and Hirsch, P. R. 1997. Occurrence of flavonoids and nucleosides in agricultural soils. Applied and Environmental Microbiology. 63 (11), pp. 4573-4577. https://doi.org/10.1128/aem.63.11.4573-4577.1997

The role of available metal ions in the transfer of mobile genetic elements

C2 - Non-edited contributions to conferences

Lawlor, K., Barbosa-Jefferson, V., Chaudri, A., McGrath, S. P. and Hirsch, P. R. 1997. The role of available metal ions in the transfer of mobile genetic elements. Abstracts 3rd ESF Workshop on Molecular Biology and Ecology of Plasmid-Mediated Gene Transfer, Cuenca, 12-16 September 1997 .

Genetic basis of wheat roots colonisation by pseudomonads

C2 - Non-edited contributions to conferences

Curnow, P. and Hirsch, P. R. 1997. Genetic basis of wheat roots colonisation by pseudomonads. Abstracts UK Soil:Plant:Microbe Interactions Initiative 3rd Annual Meeting, Oxford, 17-19 March 1997 .

Characterization of low and high affinity nitrate cotransporters expressed in Xenopus oocytes

C2 - Non-edited contributions to conferences

Zhou, J-J., Theodoulou, F. L. and Miller, A. J. 1997. Characterization of low and high affinity nitrate cotransporters expressed in Xenopus oocytes. Abstracts Phytosfere '97, Dublin, 23-25 April 1997 .

Expression in Xenopus oocytes and kinetic characterization of known sucrose carriers

C2 - Non-edited contributions to conferences

Zhou, J-J. and Miller, A. J. 1997. Expression in Xenopus oocytes and kinetic characterization of known sucrose carriers. Abstracts Meeting of EC Source Sink Projects, Pisa, 10-13 September 1997 .

Functional expression of the crnA high affinity nitrate transporter of Aspergillus nidulans in Xenopus oocytes

C2 - Non-edited contributions to conferences

Zhou, J-J., Trueman, L. J., Theodoulou, F. L., Forde, B. G. and Miller, A. J. 1997. Functional expression of the crnA high affinity nitrate transporter of Aspergillus nidulans in Xenopus oocytes. Abstracts SEB Plant Transport Group Workshop, Glasgow, 8-10 September 1997 . pp. N6

Is K+ transport into meristematic cells of barley roots maintained under severe K+ deprivation?

C2 - Non-edited contributions to conferences

Walker, D. J. and Miller, A. J. 1997. Is K+ transport into meristematic cells of barley roots maintained under severe K+ deprivation? Abstracts SEB Plant Transport Group Workshop, Glasgow, 8-10 September 1997 . pp. N1

Effects of salinity on intracellular ion compartmentation

C2 - Non-edited contributions to conferences

Cuin, T. A., Laurie, S. A., Miller, A. J. and Leigh, R. A. 1997. Effects of salinity on intracellular ion compartmentation. Abstracts SEB Plant Transport Group Workshop, Glasgow, 8-10 September 1997 . pp. S7

Salt tolerance of barley: root-shoot transport of sodium

C2 - Non-edited contributions to conferences

Carden, D. and Miller, A. J. 1997. Salt tolerance of barley: root-shoot transport of sodium. Abstracts SEB Plant Transport Group Workshop, Glasgow, 8-10 September 1997 . pp. S8

A kinetic model with ordered cytoplasmic dissociation for SUC1, an Arabidopsis H+/sucrose cotransporter expressed in Xenopus oocytes

A - Papers appearing in refereed journals

Zhou, J-J., Theodoulou, F. L., Sauer, N., Sanders, D. and Miller, A. J. 1997. A kinetic model with ordered cytoplasmic dissociation for SUC1, an Arabidopsis H+/sucrose cotransporter expressed in Xenopus oocytes. Journal Of Membrane Biology. 159, pp. 113-125.

Genetic exchange between field rhizobia mediated by plasmids and bacteriophage

C2 - Non-edited contributions to conferences

Mendum, T. A. and Hirsch, P. R. 1996. Genetic exchange between field rhizobia mediated by plasmids and bacteriophage. Proceedings EC DG XII Biotechnology Final Sectorial Meeting on Ecological Implications of Biotechnology, Norwich, 3-6 September 1996 .

Rhizobial genetic elements and the extent of genetic interactions in the field

C2 - Non-edited contributions to conferences

Hirsch, P. R. and Mendum, T. A. 1996. Rhizobial genetic elements and the extent of genetic interactions in the field. Proceedings 5th International Symposium on Bacterial Genetics and Ecology, Nafplion, Greece, 25-29 May 1996 . pp. 175

Molecular tools for monitoring bacterial gene transfer in the field

C1 - Edited contributions to conferences/learned societies

Hirsch, P. R., Young, P., Amarger, N., Toro, N., Lotz, W. and Selbitschka, W. 1996. Molecular tools for monitoring bacterial gene transfer in the field. Proceedings EC DG XII Biotechnology Final Sectorial Meeting on Ecological Implications of Biotechnology, Norwich, 3-6 September 1996 .

Genetic basis of wheat roots colonisation by pseudomonads

C2 - Non-edited contributions to conferences

Curnow, P. and Hirsch, P. R. 1996. Genetic basis of wheat roots colonisation by pseudomonads. Abstracts 8th International Congress on Molecular Plant-Microbe Interactions, Knoxville, 14-19 July 1996 .

Plant genotype, micronutrient fertilization and take-all infection influence bacterial populations in the rhizosphere of wheat

A - Papers appearing in refereed journals

Rengel, Z., Gutteridge, R. J., Hirsch, P. R. and Hornby, D. 1996. Plant genotype, micronutrient fertilization and take-all infection influence bacterial populations in the rhizosphere of wheat. Plant and Soil. 183 (2), pp. 269-277. https://doi.org/10.1007/BF00011442

Population dynamics of indigenous and genetically modified rhizobia in the field

A - Papers appearing in refereed journals

Hirsch, P. R. 1996. Population dynamics of indigenous and genetically modified rhizobia in the field. New Phytologist. 133 (1), pp. 159-171. https://doi.org/10.1111/j.1469-8137.1996.tb04351.x

Know your genes

G - Articles in popular magazines and other technical publications

Hirsch, P. R. 1996. Know your genes. Chemistry & Industry. 23, pp. 935-937.

Characterization of low and high affinity nitrogen cotransporters expressed in Xenopus oocytes

C2 - Non-edited contributions to conferences

Zhou, J-J., Theodoulou, F. L., Muldin, I., Ingemarsson, B. and Miller, A. J. 1996. Characterization of low and high affinity nitrogen cotransporters expressed in Xenopus oocytes. Abstracts SEB Plant Transport Group Workshop, Saltash, 23-25 September 1996 .

Potassium homeostasis in vacuolate plant cells

A - Papers appearing in refereed journals

Walker, D. J., Leigh, R. A. and Miller, A. J. 1996. Potassium homeostasis in vacuolate plant cells. Proceedings of the National Academy of Sciences. 93 (19), pp. 10510-10514.

Determination of pH in the digestive system of the slug Deroceras reticulatum (Muller) using ion-selective microelectrodes

A - Papers appearing in refereed journals

Walker, A. J., Miller, A. J., Glen, D. M. and Shewry, P. R. 1996. Determination of pH in the digestive system of the slug Deroceras reticulatum (Muller) using ion-selective microelectrodes. Journal of Molluscan Studies. 62, pp. 387-390.

Characterisation of high-affinity nitrate transporter homologues from higher plants

C2 - Non-edited contributions to conferences

Trueman, L. J., Dunn, S. M., Jennings, A. J., Onyeocha, I., Theodoulou, F. L., Zhang, H., Zhou, J-J., Miller, A. J. and Forde, B. G. 1996. Characterisation of high-affinity nitrate transporter homologues from higher plants. Abstracts SEB Plant Transport Group Workshop, Saltash, 23-25 September 1996 .

Nitrate transport and compartmentation in cereal root cells

A - Papers appearing in refereed journals

Miller, A. J. and Smith, S. J. 1996. Nitrate transport and compartmentation in cereal root cells. Journal of Experimental Botany. 47, pp. 843-854.

Development of a new Na+-selective microelectrode to measure intracellular Na distribution in barley

C2 - Non-edited contributions to conferences

Carden, D., Flowers, T. and Miller, A. J. 1996. Development of a new Na+-selective microelectrode to measure intracellular Na distribution in barley. Abstracts SEB Plant Transport Group Workshop, Saltash, 23-25 September 1996 .

PCR-based molecular discrimination of Verticillium chlamydosporium isolates

A - Papers appearing in refereed journals

Arora, D. K., Hirsch, P. R. and Kerry, B. R. 1996. PCR-based molecular discrimination of Verticillium chlamydosporium isolates. Mycological Research. 100 (7), pp. 801-809. https://doi.org/10.1016/S0953-7562(96)80025-6

Bacteriophages and genetically modified rhizobia in the field

C2 - Non-edited contributions to conferences

Hirsch, P. R. and Mendum, T. A. 1996. Bacteriophages and genetically modified rhizobia in the field. Abstracts ESF Network Workshop on Molecular Biology and Ecology of Plasmid-Mediated Gene Transfer, Potsdam, 28 June-1 July 1996 . pp. 21

Potential manipulation of crop rhizosphere

B - Book chapters etc edited externally

Hirsch, P. R. 1996. Potential manipulation of crop rhizosphere. in: Shewry, P. R. and Pierpoint, W. S. (ed.) Genetic engineering of crop plants for resistance to pests and diseases British Crop Protection Council (BCPC). pp. 95-97

Population dynamics of indigenous and genetically modified rhizobia in the field

C2 - Non-edited contributions to conferences

Hirsch, P. R. 1995. Population dynamics of indigenous and genetically modified rhizobia in the field. Abstracts Molecular Approaches to the Study of Plant-Microbe Symbioses: 1st New Phytologist Conference, York, 13-15 November 1995 . Wiley. https://doi.org/10.1111/j.1469-8137.1996.tb04351.x

Monitoring genetically manipulated rhizobia in field soil samples

C2 - Non-edited contributions to conferences

Hirsch, P. R., Nicholson, P. and Cullen, D. 1995. Monitoring genetically manipulated rhizobia in field soil samples. Abstracts Molecular Monitoring of Environmental Samples. Society for Applied Bacteriology Demonstration Meeting, London, 13 September 1995 .

Acquisition of genes from indigenous bacteria by inoculant strains at long-term release sites

D1 - Technical reports: non-confidential

Hirsch, P. R. 1995. Acquisition of genes from indigenous bacteria by inoculant strains at long-term release sites. European Commission, Luxembourg.

Kinetic characterisation and mutational analysis of STP1, an Arabidopsis H=/hexose cotransporter expressed in Xenopus oocytes

C2 - Non-edited contributions to conferences

Theodoulou, F. L., Zhou, J-J., Miller, A. J. and Sanders, D. 1995. Kinetic characterisation and mutational analysis of STP1, an Arabidopsis H=/hexose cotransporter expressed in Xenopus oocytes. Abstracts International Conference on the Transport of Photoassimiltes, Canterbury, 13-17 August 1995 . pp. 35

Monitoring genetically modified microorganisms following field release

C1 - Edited contributions to conferences/learned societies

Hirsch, P. R. 1995. Monitoring genetically modified microorganisms following field release. Unanswered Safety Questions when Employing GMO's. CCRO Workshop Proceedings, Noordwijkerhout, the Netherlands, 2-4 May 1995 . pp. 117-121

Nitrogen in plant cells

G - Articles in popular magazines and other technical publications

Miller, A. J. 1995. Nitrogen in plant cells. Biological Sciences Review. 8, pp. 34-37.

Characterization of low affinity nitrogen transporters expressed in Xenopus oocytes

C2 - Non-edited contributions to conferences

Zhou, J-J., Theodoulou, F. L., Muldin, I., Ingemarsson, B. and Miller, A. J. 1995. Characterization of low affinity nitrogen transporters expressed in Xenopus oocytes. Abstracts 4th International Symposium on Inorganic Nitrogen Assimilation, Darmstadt, 23-28 July 1995 . pp. 144

Xenopus oocytes as a heterologous expression system

B - Book chapters etc edited externally

Theodoulou, F. L. and Miller, A. J. 1995. Xenopus oocytes as a heterologous expression system. in: Jones, H. (ed.) Methods in molecular biology vol. 49: plant gene transfer and expression protocols Humana Press, Totowa. pp. 317-340

Homeostasis of cytosolic nitrate in barley root cells: measurements with triple-barrelled ion-selective microelectrodes

C2 - Non-edited contributions to conferences

Smith, S. J., Van Der Leij, M. and Miller, A. J. 1995. Homeostasis of cytosolic nitrate in barley root cells: measurements with triple-barrelled ion-selective microelectrodes. Abstracts 4th International Symposium on Inorganic Nitrogen Assimilation, Darmstadt, 23-28 July 1995 . pp. 118

Nitrate and potassium compartmentation in nitrogen and potassium starved barley root cells

C1 - Edited contributions to conferences/learned societies

Miller, A. J., Walker, D. J. and Smith, S. J. 1995. Nitrate and potassium compartmentation in nitrogen and potassium starved barley root cells. Leigh, R. A. and Blake-Kalff, M. M. A. (ed.) Proceedings 2nd Stessnet Conference, Salsomaggiore, Italy, 21-23 September 1995 . pp. 263-268

Ion-selective microelectrodes for measurement of intracellular ion concentrations

B - Book chapters etc edited externally

Miller, A. J. 1995. Ion-selective microelectrodes for measurement of intracellular ion concentrations. in:

Detection of microbial DNA sequences by colony hybridization

B - Book chapters etc edited externally

Hirsch, P. R. 1995. Detection of microbial DNA sequences by colony hybridization. in: Molecular microbial ecology manual Kluwer Academic Publishers, Dordrecht, Netherlands.

Characterization of low affinity nitrogen cotransporters expressed in Xenopus oocytes

C2 - Non-edited contributions to conferences

Zhou, J-J., Theodoulou, F. L., Muldin, I., Ingemarsson, B. and Miller, A. J. 1995. Characterization of low affinity nitrogen cotransporters expressed in Xenopus oocytes. Abstracts 10th International Workshop on Plant Membrane Biology, Regensberg, August 1995 . pp. R38

Cytosolic potassium homeostasis in barley root cells: measurements with triple-barrelled ion-selective microelectrodes

C2 - Non-edited contributions to conferences

Walker, D. J., Leigh, R. A. and Miller, A. J. 1995. Cytosolic potassium homeostasis in barley root cells: measurements with triple-barrelled ion-selective microelectrodes. Abstracts 10th International Workshop on Plant Membrane Biology, Regensberg, August 1995 . pp. R39

Simultaneous measurement of intracellular pH and K+ or NO3- in barley root cells using triple-barrelled ion-selective microelectrodes

A - Papers appearing in refereed journals

Walker, D. J., Smith, S. J. and Miller, A. J. 1995. Simultaneous measurement of intracellular pH and K+ or NO3- in barley root cells using triple-barrelled ion-selective microelectrodes. Plant Physiology. 108, pp. 743-751.

Kinetic characterization and mutational analysis of STP1, an Arabidopsis H+/hexose cotransporter expressed in Xenopus oocytes

C2 - Non-edited contributions to conferences

Theodoulou, F. L., Zhou, J-J., Sanders, D. and Miller, A. J. 1995. Kinetic characterization and mutational analysis of STP1, an Arabidopsis H+/hexose cotransporter expressed in Xenopus oocytes. Abstracts 10th International Workshop on Plant Membrane Biology, Regensberg, August 1995 . pp. R01

Xenopus oocytes as a heterologous expression system for plant proteins

A - Papers appearing in refereed journals

Theodoulou, F. L. and Miller, A. J. 1995. Xenopus oocytes as a heterologous expression system for plant proteins. Molecular Biotechnology. 3, pp. 101-115.

Cytosolic nitrate homeostasis in barley root cells: measurements with triple-barrelled ion-selective microelectrodes

C2 - Non-edited contributions to conferences

Smith, S. J. and Miller, A. J. 1995. Cytosolic nitrate homeostasis in barley root cells: measurements with triple-barrelled ion-selective microelectrodes. Abstracts 10th International Workshop on Plant Membrane Biology, Regensberg, August 1995 . pp. R37

Construction and characterization of a Rhizobium leguminosarum biovar viciae strain designed to assess horizontal gene transfer in the environment

A - Papers appearing in refereed journals

Selbitschka, W., Jording, D., Nieman, S., Schmidt, R., Puhler, A., Mendum, T. A. and Hirsch, P. R. 1995. Construction and characterization of a Rhizobium leguminosarum biovar viciae strain designed to assess horizontal gene transfer in the environment. FEMS Microbiology Letters. 128, pp. 255-263. https://doi.org/10.1111/j.1574-6968.1995.tb07533.x

Targets for introduction of pests and disease resistance into crops by genetic engineering: report of a review funded by MAFF

D1 - Technical reports: non-confidential

Pierpoint, W. S., Hughes, K. J. D., Shewry, P. R., Burrows, P. R., Henderson, I. F., Hirsch, P. R., Hughes , K. J. D., James, S., Lazzeri, P. A., Miflin, B. J., Plumb, R. T., Glen, D. M., Hargreaves, J. A., Elphinsone, J. G. and Stead, D. E. 1995. Targets for introduction of pests and disease resistance into crops by genetic engineering: report of a review funded by MAFF. Institute for Arable Crops Research (IACR) , Long Ashton Research Station (LARS), Bristol. https://doi.org/10.23637/rothamsted.87224

Conjugative plasmids in Rhizobium

C2 - Non-edited contributions to conferences

Hirsch, P. R. and Mendum, T. A. 1995. Conjugative plasmids in Rhizobium. Abstracts ESF Workshop on Plasmid Mediated Gene Transfer, University of Birmingham, 12-15 May 1995 .

The regulation of nitrate uptake, compartmentation and assimilation in barley

C2 - Non-edited contributions to conferences

Dunn, S. M., Trueman, L. J., Jennings, A., Miller, A. J., Lazzeri, P. A. and Forde, B. G. 1995. The regulation of nitrate uptake, compartmentation and assimilation in barley. Abstracts BBSRC Biochemistry of Metabolic Regulation in Plants (BOMRIP) Meeting, Manchester, 11-13 June 1995 .

Field release of genetically modified Rhizobia in the UK

C1 - Edited contributions to conferences/learned societies

Hirsch, P. R., Mendum, T. A., Jording, D. and Selbitschka, W. 1995. Field release of genetically modified Rhizobia in the UK. Jones, D. D. (ed.) Proceedings 3rd International Symposium on the Biosafety Results of Field Tests of GM Plants and Microorganisms, Monterey, 13-16 November 1994 . pp. 407-417

Potassium homeostasis in barley root cells: measurements with triple-barrelled ion selective microelectrodes

C2 - Non-edited contributions to conferences

Miller, A. J., Walker, D. J. and Leigh, R. A. 1995. Potassium homeostasis in barley root cells: measurements with triple-barrelled ion selective microelectrodes.

Acquisition of genes from indigenous bacteria by inoculant strains at long-term release sites

C2 - Non-edited contributions to conferences

Hirsch, P. R., Young, P., Amarger, N., Toro, N., Lotz, W. and Selbitschka, W. 1994. Acquisition of genes from indigenous bacteria by inoculant strains at long-term release sites. Proceedings Biotech Sectorial Meeting on Microbial Ecology and Biosafety, Cork, 27-29 September 1994 . pp. 27-28

Investigating bacterial root colonization in soils with long-term wheat cultivation

C2 - Non-edited contributions to conferences

Hirsch, P. R. and Ross, G. 1994. Investigating bacterial root colonization in soils with long-term wheat cultivation. Abstracts 7th International Symposium on Molecular Plant-Microbe Interactions, Edinburgh, 26 June-1 July 1994 . pp. 65

Field release of genetically modified Rhizobium leguminosarum biovar viciae

C2 - Non-edited contributions to conferences

Hirsch, P. R. and Mendum, T. A. 1994. Field release of genetically modified Rhizobium leguminosarum biovar viciae. Proceedings Biotech Sectorial Meeting on Microbial Ecology and Biosafety, Cork, 27-29 September 1994 . pp. 29

The extent of gene transfer in deliberate release experiments

C2 - Non-edited contributions to conferences

Hirsch, P. R. 1994. The extent of gene transfer in deliberate release experiments. Proceedings Horizontal Gene Transfer Mechanisms and Implications, Bielefeld, 25-27 July 1994 .

The heterologous expression of H+-coupled transporters in Xenopus oocytes

B - Book chapters etc edited externally

Miller, A. J., Smith, S. J. and Theodoulou, F. L. 1994. The heterologous expression of H+-coupled transporters in Xenopus oocytes. in: Leigh, R. A., Sanders, D. and Blatt, M. R. (ed.) Membrane transport in plants and fungi: molecular mechanisms and control Company of Biologists, Cambridge. pp. 167-177

Comparison of the chemical changes in the rhizosphere of the nickel hyperaccumulator Alyssum murale with the non-accumulator Raphanus sativus

A - Papers appearing in refereed journals

Bernal, M. P., McGrath, S. P., Miller, A. J. and Baker, A. J. M. 1994. Comparison of the chemical changes in the rhizosphere of the nickel hyperaccumulator Alyssum murale with the non-accumulator Raphanus sativus. Plant and Soil. 164 (2), pp. 251-259. https://doi.org/10.1007/BF00010077

Survival and dispersion of genetically modified rhizobia in the field and genetic interactions with native strains

A - Papers appearing in refereed journals

Hirsch, P. R. and Spokes, J. D. 1994. Survival and dispersion of genetically modified rhizobia in the field and genetic interactions with native strains. FEMS Microbiology Ecology. 15, pp. 147-160. https://doi.org/10.1111/j.1574-6941.1994.tb00239.x

Disruptive effects of protein on performance of liquid membrane-based ion-selective microelectrodes

A - Papers appearing in refereed journals

Coombs, H. V., Miller, A. J. and Sanders, D. 1994. Disruptive effects of protein on performance of liquid membrane-based ion-selective microelectrodes. American Journal of Physiology. 267, pp. C1027-C1035.

Expression of nitrate transporters in Xenopus oocytes

C2 - Non-edited contributions to conferences

Zhou, J-J., Theodoulou, F. L. and Miller, A. J. 1994. Expression of nitrate transporters in Xenopus oocytes. Abstracts Society for Experimental Biology Plant Transport Group Workshop, Newcastle, September 1994 .

An investigation of K+ homeostasis in barley root cells using triple-barrelled K+- and pH-selective microelectrodes

C2 - Non-edited contributions to conferences

Walker, D. J., Leigh, R. A., Miller, A. J. and Black, C. R. 1994. An investigation of K+ homeostasis in barley root cells using triple-barrelled K+- and pH-selective microelectrodes. Abstracts Society for Experimental Biology Plant Transport Group Workshop, Newcastle, September 1994 .

Remobilization of stored nitrate in barley root cells

C2 - Non-edited contributions to conferences

Van Der Leij, M. and Miller, A. J. 1994. Remobilization of stored nitrate in barley root cells. Abstracts Society for Experimental Biology Plant Transport Group Workshop, Newcastle, September 1994 .

Kinetics of STP1- an Arabidopsis hexose/proton cotransporter expressed in Xenopus oocytes

C2 - Non-edited contributions to conferences

Theodoulou, F. L., Zhou, J-J., Smith, S. J., Sanders, D. and Miller, A. J. 1994. Kinetics of STP1- an Arabidopsis hexose/proton cotransporter expressed in Xenopus oocytes. Abstracts Society for Experimental Biology Plant Transport Group Workshop, Newcastle, September 1994 .

Structure and function of STPI, the Arabidopsis H+/glucose transporter expressed in Xenopus oocytes

C2 - Non-edited contributions to conferences

Miller, A. J., Parry, M. A. J., Sanders, D. and Theodoulou, F. L. 1994. Structure and function of STPI, the Arabidopsis H+/glucose transporter expressed in Xenopus oocytes. Abstracts BBSRC Plant Molecular Biology II Mid-term Meeting, University of East Anglia, 6-8 July 1994 . pp. 30

Compartmentation of nutrient ions in relation to supply and growth

B - Book chapters etc edited externally

Leigh, R. A., Storey, R., Miller, A. J. and Zhen, R-G. 1994. Compartmentation of nutrient ions in relation to supply and growth. in: Smith, C. J., Zocchi, G., Chiatante, D. and Gallan, J. (ed.) Biochemical mechanisms involved in growth regulation Clarendon Press, Oxford. pp. 63-77

Cloning and analysis of a putative high affinity nitrate transporter from barley

C2 - Non-edited contributions to conferences

Trueman, L. J., Theodoulou, F. L., Miller, A. J. and Forde, B. G. 1994. Cloning and analysis of a putative high affinity nitrate transporter from barley. Abstracts 4th International Congress of Plant Molecular Biology, Amsterdam, 20-24 June 1994 .

Ion-selective microelectrodes

B - Book chapters etc edited externally

Miller, A. J. 1994. Ion-selective microelectrodes. in: Harris, N. and Oparka, K. J. (ed.) Plant cell biology: a practical approach Oxford University Press (OUP) Oxford. pp. 283-296

Remobilisation of nitrate in barley root cells

C2 - Non-edited contributions to conferences

Van Der Leij, M. and Miller, A. J. 1993. Remobilisation of nitrate in barley root cells. Abstracts AFRC Meeting on Plant and Soil Nitrogen Metabolism, Silsoe, December 1993 . pp. 12

Compartmentation of nitrate in barley root cells growing at different nitrogen levels

C2 - Non-edited contributions to conferences

Miller, A. J. and Smith, S. J. 1993. Compartmentation of nitrate in barley root cells growing at different nitrogen levels. Abstracts AFRC Meeting on Plant and Soil Nitrogen Metabolism, Silsoe, December 1993 . pp. 11

Acquisition of genes from indigenous bacteria by inoculant strains at long-term release sites

C2 - Non-edited contributions to conferences

Hirsch, P. R., Young, P., Amarger, N., Toro, N., Lotz, W. and Selbitschka, W. 1993. Acquisition of genes from indigenous bacteria by inoculant strains at long-term release sites. Abstracts BRIDGE/BIOTECH Final Sectorial Meeting on Biosafety and First Sectorial Meeting on Microbial Ecology, Granada, October 1993 . pp. 142

Investigating gene transfer in soil using genetically modified rhizobia

C2 - Non-edited contributions to conferences

Hirsch, P. R. and Mendum, T. A. 1993. Investigating gene transfer in soil using genetically modified rhizobia. Abstracts BRIDGE/BIOTECH Final Sectorial Meeting on Biosafety and First Sectorial Meeting on Microbial Ecology, Granada, October 1993 . pp. 143

Behaviour of genetically modified rhizobia in the field

C2 - Non-edited contributions to conferences

Hirsch, P. R. 1993. Behaviour of genetically modified rhizobia in the field. Abstracts BAGECO 4: 4th Symposium on Bacterial Genetics and Ecology, Wageningen, November 1993 .

Techniques for monitoring rhizobia in the field

C2 - Non-edited contributions to conferences

Hirsch, P. R., Nicholson, P. S. and Cullen, D. W. 1993. Techniques for monitoring rhizobia in the field. Abstracts Combined EERO and DLO-AFRC Workshop, Wageningen, November 1993 .

Molecular approaches to monitoring bacteria in soil

C2 - Non-edited contributions to conferences

Hirsch, P. R., Nicholson, P. S. and Cullen, D. W. 1993. Molecular approaches to monitoring bacteria in soil. Abstracts. Beyond the Biomass - Compositional and Functional Analysis of Soil Microbial Communities, Wye College, March 1993 .

Heavy metals from past applications of sewage sludge decrease the genetic diversity of Rhizobium leguminosarum biovar Trifolii populations

A - Papers appearing in refereed journals

Hirsch, P. R., Jones, M. J., McGrath, S. P. and Giller, K. E. 1993. Heavy metals from past applications of sewage sludge decrease the genetic diversity of Rhizobium leguminosarum biovar Trifolii populations. Soil Biology and Biochemistry. 25 (11), pp. 1485-1490. https://doi.org/10.1016/0038-0717(93)90003-T

Regulation of intracellular potassium levels in barley root cells

C2 - Non-edited contributions to conferences

Walker, D. J., Miller, A. J., Leigh, R. A. and Black, C. R. 1993. Regulation of intracellular potassium levels in barley root cells. Proceedings SEB Symposium Membrane Transport in Plants and Fungi, Wye College, September 1993 . pp. 31

The heterologous expression of H+ cotransport proteins in Xenopus oocytes

C2 - Non-edited contributions to conferences

Miller, A. J., Smith, S. and Theodoulou, F. L. 1993. The heterologous expression of H+ cotransport proteins in Xenopus oocytes. Proceedings SEB Symposium Membrane Transport in Plants and Fungi, Wye College, September 1993 . pp. 8

Nitrate concentrations in cell compartments in relation to nitrogen nutrition

C2 - Non-edited contributions to conferences

Miller, A. J. and Smith, S. 1993. Nitrate concentrations in cell compartments in relation to nitrogen nutrition. Proceedings 1st Stressnet Meeting, Manchester University, April 1993 .

Compartmentation of nitrate in barley root cells

C2 - Non-edited contributions to conferences

Miller, A. J., Zhen, R-G. and Smith, S. J. 1992. Compartmentation of nitrate in barley root cells. Abstracts 3rd International Symposium on Inorganic Nitrogen Assimilation, Tiberias Israel, September 1992 . pp. A38

Measurement of intracellular free magnesium in plant cells using magnesium-selective microelectrodes

C2 - Non-edited contributions to conferences

Coombs, H. V., Miller, A. J. and Sanders, D. 1992. Measurement of intracellular free magnesium in plant cells using magnesium-selective microelectrodes. Abstracts Society for Experimental Biology Plant Transport Group Discussion Meeting, Oxford, September 1992 . pp. 5

Intracellular measurement of K+ in barley root epidermal cells using K+-selective microelectrodes

C2 - Non-edited contributions to conferences

Walker, D. J., Miller, A. J., Leigh, R. A. and Black, C. R. 1992. Intracellular measurement of K+ in barley root epidermal cells using K+-selective microelectrodes. Abstracts Society for Experimental Biology Plant Transport Group Discussion Meeting, Oxford September 1992 . pp. 29

Compartmentation of nitrate in root cells

C2 - Non-edited contributions to conferences

Miller, A. J. and Smith, S. J. 1992. Compartmentation of nitrate in root cells. Abstracts Society for Experimental Biology Plant Transport Group Discussion Meeting, Oxford, September 1992 . pp. 18

Functional expression of a plant plasma membrane transporter in Xenopus oocytes

C2 - Non-edited contributions to conferences

Boorer, K. J., Forde, B. G., Leigh, R. A. and Miller, A. J. 1992. Functional expression of a plant plasma membrane transporter in Xenopus oocytes. Proceedings 9th International Workshop on Plant Membrane Biology, Monterey, July 1992 . Wiley. https://doi.org/10.1016/0014-5793(92)80431-F

Monitoring survival of genetically-modified Rhizobium in the field

C1 - Edited contributions to conferences/learned societies

Nicholson, P. S., Jones, M. J. and Hirsch, P. R. 1992. Monitoring survival of genetically-modified Rhizobium in the field. Stewart-Tull, D. E. S. and Sussman, M. (ed.) The release of genetically modified microorganisms. Plenum Press, New York. pp. 217-219

Functional expression of a plant plasma membrane transporter in Xenopus oocytes

C2 - Non-edited contributions to conferences

Boorer, K. J., Forde, B. G., Leigh, R. A. and Miller, A. J. 1992. Functional expression of a plant plasma membrane transporter in Xenopus oocytes. American Society of Plant Biologists (ASPB).

Magnesium homeostasis

C2 - Non-edited contributions to conferences

Coombs, H. V., Sanders, D. and Miller, A. J. 1992. Magnesium homeostasis. Oxford University Press (OUP) Oxford.

Functional expression of a plant plasma membrane transporter in Xenopus oocytes

C2 - Non-edited contributions to conferences

Boorer, K. J., Forde, B. G., Leigh, R. A. and Miller, A. J. 1992. Functional expression of a plant plasma membrane transporter in Xenopus oocytes. Wiley.

Measurement of intracellular free magnesium in plant cells using magnesium selective microelectrodes

C2 - Non-edited contributions to conferences

Coombs, H. V., Sanders, D. and Miller, A. J. 1992. Measurement of intracellular free magnesium in plant cells using magnesium selective microelectrodes.

Nitrate is actively transported at the tonoplast of barley root cells.

C2 - Non-edited contributions to conferences

Miller, A. J., Zhen, R-G., Leigh, R. A. and Smith, S. J. 1992. Nitrate is actively transported at the tonoplast of barley root cells. Proceedings 9th International Workshop on Plant Membrane Biology, Monterey, July 1992 . pp. 190

The mechanism of nitrate transport across the tonoplast of barley root cells

A - Papers appearing in refereed journals

Miller, A. J. and Smith, S. J. 1992. The mechanism of nitrate transport across the tonoplast of barley root cells. Planta. 187, pp. 554-557.

Crop plants effects on field populations of rhizobia

C2 - Non-edited contributions to conferences

Hirsch, P. R., Jones, M. and Spokes, J. 1992. Crop plants effects on field populations of rhizobia. Program and Abstracts Sixth International Symposium on Molecular Plant-Microbe Interactions, University of Washington, 1992. pp. Abstract no. 75

The identification and classification of Rhizobium and Bradyrhizobium

B - Book chapters etc edited externally

Hirsch, P. R. 1992. The identification and classification of Rhizobium and Bradyrhizobium. in: Skinner, F. A., Jones, D. and Board, R. G. (ed.) Identification methods in applied and environmental microbiology Blackwell Scientific Publications, Oxford. pp. 45-65

Compartmentation of nitrate

C2 - Non-edited contributions to conferences

Miller, A. J., Zhen, R-G. and Smith, S. J. 1992. Compartmentation of nitrate. Oxford University Press (OUP) Oxford.

Functional expression of a plant plasma membrane transporter in Xenopus oocytes

A - Papers appearing in refereed journals

Boorer, K. J., Forde, B. G., Leigh, R. A. and Miller, A. J. 1992. Functional expression of a plant plasma membrane transporter in Xenopus oocytes. FEBS Letters. 302, pp. 166-168. https://doi.org/10.1016/0014-5793(92)80431-F

A comparison of nitrate-selective microelectrodes made with different nitrate sensors and the measurement of intracellular nitrate activities in cells of excised barley roots

A - Papers appearing in refereed journals

Zhen, R-G., Smith, S. J. and Miller, A. J. 1992. A comparison of nitrate-selective microelectrodes made with different nitrate sensors and the measurement of intracellular nitrate activities in cells of excised barley roots. Journal of Experimental Botany. 43, pp. 131-138.

Assessing the risks involved in the release of genetically manipulated microorganisms

D1 - Technical reports: non-confidential

Armarger, N., Hirsch, P. R. and Klingmuller, W. 1991. Assessing the risks involved in the release of genetically manipulated microorganisms. Commission of the European Communities (CEC), Luxembourg.

Assessing the risks involved in the release of genetically manipulated microorganisms

D1 - Technical reports: non-confidential

Armarger, N., Hirsch, P. R. and Klingmuller, W. 1991. Assessing the risks involved in the release of genetically manipulated microorganisms. Commission of the European Communities (CEC), Luxembourg.

Compartmental nitrate concentrations in barley root cells measured with nitrate-selective microelectrodes and by single-cell sap sampling

A - Papers appearing in refereed journals

Zhen, R-G., Koyro, H-W., Leigh, R. A., Tomos, A. D. and Miller, A. J. 1991. Compartmental nitrate concentrations in barley root cells measured with nitrate-selective microelectrodes and by single-cell sap sampling. Planta. 185, pp. 356-361.

Cytosolic calcium homeostasis in fungi: roles of plasma membrane transport and intracellular sequestration of calcium

A - Papers appearing in refereed journals

Miller, A. J., Vogg, G. and Sanders, D. 1991. Cytosolic calcium homeostasis in fungi: roles of plasma membrane transport and intracellular sequestration of calcium. Proceedings of the National Academy of Sciences. 87 (23), pp. 9348-9352. https://doi.org/10.1073/pnas.87.23.9348

Measurement of intracellular nitrate concentrations in Chara using nitrate-selective microelectrodes

A - Papers appearing in refereed journals

Miller, A. J. and Zhen, R-G. 1991. Measurement of intracellular nitrate concentrations in Chara using nitrate-selective microelectrodes. Planta. 184, pp. 47-52.

Comparison of late Sym genes in Rhizobium species and construction of improved strains

B - Book chapters etc edited externally

Boistard, P., Hirsch, P. R., O'Connell, M., Puhler, A. and Priefer, U. B. 1990. Comparison of late Sym genes in Rhizobium species and construction of improved strains. in: Vassarotti, A. and Magnien, G. (ed.) Biotechnology R & D in the EC (BAP 1985-1989). Vol II Detailed Final Report of BAP Contractors Elsevier, Paris.

Comparison of late Sym genes in Rhizobium species and construction of improved strains

B - Book chapters etc edited externally

Boistard, P., Hirsch, P. R., O'Connell, M. and Puhler, A. 1990. Comparison of late Sym genes in Rhizobium species and construction of improved strains. in: Vassarotti, A. and Magnien, E. (ed.) Biotechnology R & D in the EC (BAP 1985-1989). Vol I Catalogue of BAP Achievements Elsevier, Paris.

Assessing the risks involved in the release of genetically manipulated microorganisms

B - Book chapters etc edited externally

Amarger, N., Hirsch, P. R. and Klingmuller, W. 1990. Assessing the risks involved in the release of genetically manipulated microorganisms. in: Vassarotti, A. and Magnien, E. (ed.) Biotechnology R & D in the EC (BAP 1985-1989). Vol I Catalogue of BAP Achievements Elsevier, Paris.

Factors limiting gene transfer in bacteria

B - Book chapters etc edited externally

Hirsch, P. R. 1990. Factors limiting gene transfer in bacteria. in: Fry, J. C. and Day, M. J. (ed.) Bacterial genetics in natural environments Chapman & Hall, London. pp. 31-40

Assessing the risks involved in the release of genetically manipulated microorganisms

D1 - Technical reports: non-confidential

Amarger, N., Hirsch, P. R. and Klingmuller, W. 1990. Assessing the risks involved in the release of genetically manipulated microorganisms. Elsevier, Paris.

Measurement of intracellular nitrate in giant algal cells using nitrate-selective microelectrodes

C2 - Non-edited contributions to conferences

Miller, A. J. and Zhen, R-G. 1990. Measurement of intracellular nitrate in giant algal cells using nitrate-selective microelectrodes. Annual Meeting of the Society of Experimental Biology, Warwick, 1990. Abstract. pp. P1-4

Cytosolic free calcium homeostasis in plants

A - Papers appearing in refereed journals

Sanders, D., Miller, A. J., Blackford, S., Brosnan, J. M. and Johannes, E. 1990. Cytosolic free calcium homeostasis in plants. Current Topics in Plant Biochemistry and Physiology. 9, pp. 20-37.

The energetics of cytosolic calcium homeostasis in fungal cells

A - Papers appearing in refereed journals

Miller, A. J. and Sanders, D. 1990. The energetics of cytosolic calcium homeostasis in fungal cells. Plant Physiology and Biochemistry. 27, pp. 551-556.

The rhizobium-legume symbiosis.

G - Articles in popular magazines and other technical publications

Hirsch, P. R., O'Connell, M., Boistard, P., Puhler, A. and Priefer, U. B. 1990. The rhizobium-legume symbiosis.

Monitoring genetically manipulated rhizobial inoculants following intentional introduction in agricultural soils

C2 - Non-edited contributions to conferences

Hirsch, P. R., Spokes, J. R., Klingmuller, W., Dohler, K., Amarger, N. and Delgutte, D. 1989. Monitoring genetically manipulated rhizobial inoculants following intentional introduction in agricultural soils. Abstracts, BAP Sectional Meeting on Risk Assessment, Dijon, 3-6 December 1989.

Rhizobium meliloti FIXGHI and FIXNOQP sequences predict involvement of a specific cation pump and of a cytochrome in symbiotic nitrogen fixation

C2 - Non-edited contributions to conferences

Kahn, D., Boistard, P., David, M., Da Veran Mingot, M. L., Hirsch, P. R. and Batut, J. 1989. Rhizobium meliloti FIXGHI and FIXNOQP sequences predict involvement of a specific cation pump and of a cytochrome in symbiotic nitrogen fixation. Abstracts, CEC BAP Final Meeting: Plant Biotechnology in the European Community, Bad Honref, 19-21 December 1989. pp. 145

A novel approach for intracellular nitrate measurement.

C2 - Non-edited contributions to conferences

Miller, A. J. and Zhen, R-G. 1989. A novel approach for intracellular nitrate measurement. AFRC Meeting on Plant and Soil Nitrogen Metabolism, Lancaster, 1989, Abstract 44..

Role of plasma membrane transport in cytosolic calcium homeostasis in Neurospora .

B - Book chapters etc edited externally

Miller, A. J. and Sanders, D. 1989. Role of plasma membrane transport in cytosolic calcium homeostasis in Neurospora . in: Plant membrane transport: the current position. J. Dainty, M.I. de Michelis, E. Marre & F. Rasi-Caldogno eds Elsevier, Amsterdam. pp. 273-274

The energetics of cytosolic calcium homeostasis in fungal cells.

G - Articles in popular magazines and other technical publications

Miller, A. J. and Sanders, D. 1989. The energetics of cytosolic calcium homeostasis in fungal cells. Plant Physiology and Biochemistry. 27, pp. 551-556.

Past, present and future uses of Rhizobium inoculants.

A - Papers appearing in refereed journals

Hirsch, P. R. 1989. Past, present and future uses of Rhizobium inoculants. Journal of the Science of Food and Agriculture. 50 (1), pp. 130-131. https://doi.org/10.1002/jsfa.2740500115

Absence of nitrogen fixation in clover grown on soil subject to long-term contamination with heavy metals is due to survival of only ineffective Rhizobium.

A - Papers appearing in refereed journals

Giller, K. E., McGrath, S. P. and Hirsch, P. R. 1989. Absence of nitrogen fixation in clover grown on soil subject to long-term contamination with heavy metals is due to survival of only ineffective Rhizobium. Soil Biology and Biochemistry. 21 (6), pp. 841-848. https://doi.org/10.1016/0038-0717(89)90179-X

Field release of rhizobia marked with a transposon.

C2 - Non-edited contributions to conferences

Hirsch, P. R. and Spokes, J. R. 1989. Field release of rhizobia marked with a transposon. In Abstracts International conferernce on risk assessment in agriculture biotechnology, Davis, California, 1989 .

Rhizobium meliloti fixGHI sequence predicts involvement of a specific cation pump in symbiotic nitrogen fixation.

A - Papers appearing in refereed journals

Kahn, D., David, M., Domergue, O., Daveran, M-L., Ghai, J., Hirsch, P. R. and Batut, J. 1989. Rhizobium meliloti fixGHI sequence predicts involvement of a specific cation pump in symbiotic nitrogen fixation. Journal of Bacteriology. 171 (2), pp. 929-939. https://doi.org/10.1128/jb.171.2.929-939.1989

Field release of rhizobia marked with a transposon.

G - Articles in popular magazines and other technical publications

Hirsch, P. R. and Spokes, J. R. 1988. Field release of rhizobia marked with a transposon.

Assessing the risks involved in the release of genetically manipulated microorganisms.

D1 - Technical reports: non-confidential

Hirsch, P. R. 1988. Assessing the risks involved in the release of genetically manipulated microorganisms. Commission of the European Communities (CEC), Luxembourg.

Comparison of late Sym genes in Rhizobium species and construction of improved strains.

D1 - Technical reports: non-confidential

Hirsch, P. R. 1988. Comparison of late Sym genes in Rhizobium species and construction of improved strains. Commission of the European Communities (CEC), Luxembourg.

Inhibition of tonoplast H+-pyrophosphatase by calcium.

C2 - Non-edited contributions to conferences

Skiera, L., Miller, A. J., Rea, P. A. and Sanders, D. 1988. Inhibition of tonoplast H+-pyrophosphatase by calcium. Meeting of the Plant Transport Group, Bangor, 1988.

Stoichiometry of H+/Ca2+ antiport at the tonoplast is consistent with energized transport of Ca2+ into the vacuole.

C2 - Non-edited contributions to conferences

Blackford, S., Miller, A. J., Rea, P. A. and Sanders, D. 1988. Stoichiometry of H+/Ca2+ antiport at the tonoplast is consistent with energized transport of Ca2+ into the vacuole. Meeting of the Plant Transport Group, Bangor, 1988.

Suppression of N2-fixation in clover on soil contaminated with heavy metals.

C2 - Non-edited contributions to conferences

Giller, K. E., McGrath, S. P. and Hirsch, P. R. 1988. Suppression of N2-fixation in clover on soil contaminated with heavy metals. Nitrogen fixation: hundred years after. Proceedings 7th International Congress on Nitrogen Fixation. Eds. H. Bothe, F.J. de Bruijn & W.E. Newton. Stuttgart : Gustav Fischer, p. 813..

Rhizobia isolated from root nodules of clover grown on soils with heavy metal contamination.

C2 - Non-edited contributions to conferences

Hirsch, P. R., Giller, K. E. and McGrath, S. P. 1988. Rhizobia isolated from root nodules of clover grown on soils with heavy metal contamination. Nitrogen fixation: hundred years after. Proceedings 7th International Congress on Nitrogen Fixation. Eds. H. Bothe, F.J. de Bruijn & W.E. Newton. Stuttgart : Gustav Fischer, p. 816..

Gene transfer between rhizobia in controlled conditions and the field.

C2 - Non-edited contributions to conferences

Spokes, J. R. and Hirsch, P. R. 1988. Gene transfer between rhizobia in controlled conditions and the field. REGEM-1 1st International Conference on the Release of Genetically Manipulated Microorganisms, Cardiff, 1988. Abstracts ..

Effect of heavy metal contamination on the genetics of nitrogen-fixing populations of Rhizobium leguminosarum nodulating white clover.

C1 - Edited contributions to conferences/learned societies

McGrath, S. P., Hirsch, P. R. and Giller, K. E. 1988. Effect of heavy metal contamination on the genetics of nitrogen-fixing populations of Rhizobium leguminosarum nodulating white clover. Environmental contamination. Ed. A.A. Orio. Edinburgh : CEP. pp. 164-166

Rhizobium leguminosarum as a model for investigating gene transfer in soil

C1 - Edited contributions to conferences/learned societies

Hirsch, P. R. and Spokes, J. 1988. Rhizobium leguminosarum as a model for investigating gene transfer in soil. Risk assessment for deliberate releases . Ed. W. Klingmuller. Berlin : Springer. pp. 10-17

Mutation, transfer and location of symbiotic genes of Rhizobium leguminosarum biovar phaseoli by insertion of Tn5-Mob.

A - Papers appearing in refereed journals

Wang, C. L. and Hirsch, P. R. 1988. Mutation, transfer and location of symbiotic genes of Rhizobium leguminosarum biovar phaseoli by insertion of Tn5-Mob. Acta Genetica Sinica. 15, pp. 25-33.

Assessing the environmental risks of releasing genetically manipulated organisms.

G - Articles in popular magazines and other technical publications

Hirsch, P. R. 1988. Assessing the environmental risks of releasing genetically manipulated organisms.

Genetic factors in Rhizobium affecting the symbiotic carbon costs of N2 fixation and host plant biomass production.

A - Papers appearing in refereed journals

Skot, L., Hirsch, P. R. and Witty, J. F. 1986. Genetic factors in Rhizobium affecting the symbiotic carbon costs of N2 fixation and host plant biomass production. Journal of Applied Bacteriology. 61, pp. 239-246.

Gene cloning and its potential application to mycorrhizal fungi.

G - Articles in popular magazines and other technical publications

Hirsch, P. R. 1986. Gene cloning and its potential application to mycorrhizal fungi.

Host plant effects on hybrids of Rhizobium leguminosarum Biovars viceae and trifolii .

A - Papers appearing in refereed journals

Wang, C. L., Beringer, J. E. and Hirsch, P. R. 1986. Host plant effects on hybrids of Rhizobium leguminosarum Biovars viceae and trifolii . Journal of General Microbiology. 132, pp. 2063-2070. https://doi.org/10.1099/00221287-132-8-2063

Construction of a Tn5 derivative determining resistance to gentamicin and spectinomycin using a fragment cloned from R1033.

A - Papers appearing in refereed journals

Hirsch, P. R., Wang, C. L. and Woodward, M. J. 1986. Construction of a Tn5 derivative determining resistance to gentamicin and spectinomycin using a fragment cloned from R1033. Gene. 48, pp. 203-209.

Nitrogen-fixation

A - Papers appearing in refereed journals

Beringer, J. E., Hirsch, P. R. and Witty, J. F. 1985. Nitrogen-fixation. Journal of the Science of Food and Agriculture. 36 (8), pp. 651-651.

Transposon mutagenesis of Rhizobium using a derivitive of Tn5 conferring gentamicin and spectinomycin resistance.

G - Articles in popular magazines and other technical publications

Wang, C. L., Spokes, J. R., Woodward, M. J. and Hirsch, P. R. 1985. Transposon mutagenesis of Rhizobium using a derivitive of Tn5 conferring gentamicin and spectinomycin resistance.

Rothamsted Multidisciplinary Agronomy

A - Papers appearing in refereed journals

Thorne, G. N., Prew, R. D., williams, Lacey, J., Plumb, R. T., Hirsch, P. R., Church, B. M., Todd, A. D. and Carter, N. 1984. Rothamsted Multidisciplinary Agronomy. Rothamsted Experimental Station Report. 1984 (part 1), pp. 23-38.

Agrobacterium tumefaciens T-DNA in the yeast Saccharomyces cerevisiae

A - Papers appearing in refereed journals

Hirsch, P. R. and Beggs, J. D. 1984. Agrobacterium tumefaciens T-DNA in the yeast Saccharomyces cerevisiae. Molecular and General Genetics (MGG). 195 (1-2), pp. 209-214. https://doi.org/10.1007/BF00332748

A physical map of pPH1JI and pJB4JI

A - Papers appearing in refereed journals

Hirsch, P. R. and Beringer, J. E. 1984. A physical map of pPH1JI and pJB4JI. Plasmid. 12 (2), pp. 139-141. https://doi.org/10.1016/0147-619x(84)90059-3

Genetic engineering and nitrogen fixation

A - Papers appearing in refereed journals

Beringer, J. E. and Hirsch, P. R. 1984. Genetic engineering and nitrogen fixation. Biotechnology & Genetic Engineering Reviews. 1 (1), pp. 65-88. https://doi.org/10.1080/02648725.1984.10647781

Genetic adaptation to the environment. The role of plasmids in microbial ecology.

G - Articles in popular magazines and other technical publications

Beringer, J. E. and Hirsch, P. R. 1984. Genetic adaptation to the environment. The role of plasmids in microbial ecology.

Improved crop plant productivity through genetic manipulation of mycorrhizal fungi

G - Articles in popular magazines and other technical publications

Hirsch, P. R. 1984. Improved crop plant productivity through genetic manipulation of mycorrhizal fungi. Chemistry & Industry. 23 (23), pp. 833-838.

Rothamsted Multidisciplinary Agronomy

A - Papers appearing in refereed journals

Thorne, G. N., Dewar, A. M., Prew, R. D., williams, Lacey, J., Plumb, R. T., Hirsch, P. R., Church, B. M. and Todd, A. D. 1983. Rothamsted Multidisciplinary Agronomy. Rothamsted Experimental Station Report. 1983 (part 1), pp. 21-36.

Plasmid-determined Bacteriocin Production by Rhizobium leguminosarum

A - Papers appearing in refereed journals

Hirsch, P. R. 1979. Plasmid-determined Bacteriocin Production by Rhizobium leguminosarum. Microbiology. 113 (2), pp. 219-228. https://doi.org/10.1099/00221287-113-2-219