Microbiota in wheat roots evaluated by cloning of ITS1/2 rDNA and sequencing

A - Papers appearing in refereed journals

Kwasna, H., Bateman, G. L. and Ward, E. 2010. Microbiota in wheat roots evaluated by cloning of ITS1/2 rDNA and sequencing. Journal of Phytopathology. 158 (4), pp. 278-287.

AuthorsKwasna, H., Bateman, G. L. and Ward, E.
Abstract

Fungi (17 species), oomycetous organisms (four species of Pythium) and a plasmodiophorid (Polymyxa graminis) were recorded in wheat roots analysed by cloning of the total ITS1/2 rDNA and sequencing of representative clones. Roots of a fourth successive wheat crop were inhabited mostly by fungal pathogens including Gaeumannomyces graminis var. tritici, Monographella nivalis var. nivalis, Ophiosphaerella sp. and Helgardia anguioides. Roots of a first wheat crop were inhabited mostly by P. graminis and saprotrophic Pythium species. Results on fungal diversity and density were compared with those obtained by pure culture isolation and morphotyping. Only M. nivalis var. nivalis and H. anguioides were identifed in wheat roots by both the molecular and the pure culture isolation methods. New and additional evidence for the ecological roles of the species recorded is discussed.

KeywordsPlant Sciences
Year of Publication2010
JournalJournal of Phytopathology
Journal citation158 (4), pp. 278-287
Digital Object Identifier (DOI)doi:10.1111/j.1439-0434.2009.01595.x
Open accessPublished as non-open access
Funder project or codeSEF
FunderEuropean Commission - EC
Biotechnology and Biological Sciences Research Council
ISSN09311785
PublisherWiley

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