Characterisation of imidacloprid resistance mechanisms in the brown planthopper, Nilaparvata lugens Stal (Hemiptera: Delphacidae)

A - Papers appearing in refereed journals

Puinean, A. M., Denholm, I., Millar, N. S., Nauen, R. and Williamson, M. S. 2010. Characterisation of imidacloprid resistance mechanisms in the brown planthopper, Nilaparvata lugens Stal (Hemiptera: Delphacidae). Pesticide Biochemistry and Physiology. 97 (2), pp. 129-132.

AuthorsPuinean, A. M., Denholm, I., Millar, N. S., Nauen, R. and Williamson, M. S.
Abstract

Effective control of the brown planthopper, Nilaparvata lugens Stål, across rice-growing regions of Asia has been seriously compromised over the last 2 years by the appearance of widespread resistance to the neonicotinoid insecticide, imidacloprid. Sequence analysis of the ligand-binding domain of the nicotinic acetylcholine receptor α1 subunit from two field-collected resistant strains (CHN-2 and IND-11) did not reveal the Y151S point mutation previously implicated in conferring target-site resistance in this species. This result was supported by ligand-binding studies with [3H]-imidacloprid that showed no significant change in insecticide binding to isolated membranes from susceptible and resistant strains. In contrast, there was an approximate 5-fold increase in the mixed function oxidase activity for the two resistant strains suggesting that imidacloprid metabolism by increased cytochrome P450 monooxygenase activity is the major mechanism of resistance in these strains.

KeywordsNilaparvata lugens; Neonicotinoid; Imidacloprid; Metabolic resistance; Oxidase activity
Year of Publication2010
JournalPesticide Biochemistry and Physiology
Journal citation97 (2), pp. 129-132
Digital Object Identifier (DOI)doi:10.1016/j.pestbp.2009.06.008
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeCentre for Sustainable Pest and Disease Management (PDM)
Investigating resistance to neonicotinoid insecticides in insect pests
Output statusPublished
Publication dates
Online28 Jun 2009
Publication process dates
Accepted24 Jun 2009
PublisherElsevier
ISSN0048-3575

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