Determining frequencies of avirulent alleles in airborne Leptosphaeria maculans inoculum using quantitative PCR

A - Papers appearing in refereed journals

Van De Wouw, A. P., Stonard, J. F., Howlett, B. J., West, J. S., Fitt, B. D. L. and Atkins, S. D. 2010. Determining frequencies of avirulent alleles in airborne Leptosphaeria maculans inoculum using quantitative PCR. Plant Pathology. 59 (5), pp. 809-818.

AuthorsVan De Wouw, A. P., Stonard, J. F., Howlett, B. J., West, J. S., Fitt, B. D. L. and Atkins, S. D.
Abstract

Phoma stem canker (blackleg disease) of Brassica napus (oilseed rape, canola) is caused by the fungus Leptosphaeria maculans. Frequencies of avirulent alleles for loci where virulence can be associated with gene deletion (AvrLm1 and AvrLm6) were determined in samples of L. maculans airborne ascospore inoculum using quantitative PCR. The accuracy, reproducibility and limitations of detection were determined. Changes in the frequency of avirulent alleles were determined for the 2006/2007, 2007/2008 and 2008/2009 growing seasons for winter oilseed rape in the UK. The frequency of AvrLm1 remained small (between 9% and 16%), whilst the frequency of AvrLm6 fluctuated between 35% and 66%. Estimation of frequencies of avirulent alleles in airborne pathogen inoculum gives an efficient and unbiased method to assess the potential of crop cultivars with corresponding resistance genes being at risk of disease.

KeywordsAgronomy; Plant Sciences
Year of Publication2010
JournalPlant Pathology
Journal citation59 (5), pp. 809-818
Digital Object Identifier (DOI)doi:10.1111/j.1365-3059.2010.02311.x
Open accessPublished as non-open access
Funder project or codePDM
BCC
FunderAustralian Grains Research and Development Corporation, GRDC
Biotechnology and Biological Sciences Research Council
HGCA - Home Grown Cereals Authority
Sustainable Arable LINK programme
ISSN00320862
PublisherWiley

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