Multiplex reactions for the molecular detection of predation on pest and nonpest invertebrates in agroecosystems

A - Papers appearing in refereed journals

King, R. A., Moreno-Ripoll, R., Agusti, N., Shayler, S. P., Bell, J. R., Bohan, D. A. and Symondson, W. O. C. 2011. Multiplex reactions for the molecular detection of predation on pest and nonpest invertebrates in agroecosystems. Molecular Ecology Resources. 11 (2), pp. 370-373.

AuthorsKing, R. A., Moreno-Ripoll, R., Agusti, N., Shayler, S. P., Bell, J. R., Bohan, D. A. and Symondson, W. O. C.
Abstract

Species- and group-specific PCR primers were developed to study predation on pest and nonpest invertebrate species by generalist carabid predators in agroecosystems. To ensure the amplification of degraded DNA in predator gut samples, amplicons were designed to be less than 300 bp. Specificity of primers was assessed by cross-amplification against a panel of target and nontarget invertebrate species. The new primers were combined with previously published primers for slugs and collembolla in multiplex reactions to simultaneously screen each predator for the presence of multiple prey. All prey species were detected in a screen of the gut contents of field-caught predators.

KeywordsBiochemistry & Molecular Biology; Ecology; Evolutionary Biology
Year of Publication2011
JournalMolecular Ecology Resources
Journal citation11 (2), pp. 370-373
Digital Object Identifier (DOI)doi:10.1111/j.1755-0998.2010.02913.x
PubMed ID21429146
Open accessPublished as non-open access
Funder project or codeSEF
FunderBiotechnology and Biological Sciences Research Council
PublisherWiley
Grant IDBBD0011881
ISSN1755-098X

Permalink - https://repository.rothamsted.ac.uk/item/8q70y/multiplex-reactions-for-the-molecular-detection-of-predation-on-pest-and-nonpest-invertebrates-in-agroecosystems

Restricted files

Publisher's version

Under embargo indefinitely

Total views: 2

Total downloads: 1

Views this month: 0

Downloads this month: 0