Measuring soil microbial biomass using an automated procedure

A - Papers appearing in refereed journals

Joergensen, R. G., Wu, J. and Brookes, P. C. 2011. Measuring soil microbial biomass using an automated procedure. Soil Biology and Biochemistry. 43 (5), pp. 873-876.

AuthorsJoergensen, R. G., Wu, J. and Brookes, P. C.

Here we outline the development of the first automated procedure for measuring soil microbial biomass carbon (biomass C) by Fumigation–Extraction (FE) and on which this Citation Classic is based. The method (and its later variations) has been used widely since it was published. It gives essentially the same results as the Fumigation–Incubation (FI) method on which it was based and which it has now largely replaced. Analysis of the current data clearly showed that the calibration value to convert extracted organic C to biomass C (kEC = 0.45) using FE is still valid and that there was no need for a change. We also review some of the previous discussions about the method and outline future prospects for microbial biomass measurements in soil microbial ecology.

KeywordsBiomass carbon measurements; Fumigatione-Extraction; Fumigatione-Incubation
Year of Publication2011
JournalSoil Biology and Biochemistry
Journal citation43 (5), pp. 873-876
Digital Object Identifier (DOI)
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeSEF
Carbon, nutrient and energy flows through the soil microbial biomass and soil ecosystem functioning
Publisher's version
Copyright license
Publisher copyright
Output statusPublished
Publication dates
Online13 Oct 2010
Publication process dates
Accepted20 Sep 2016

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