Functional characterization and immunolocalization of odorant binding protein 1 in the lucerne plant bug, Adelphocoris lineolatus (Goeze)

A - Papers appearing in refereed journals

Gu, S-H., Wang, W-X., Wang, G-R., Zhang, X-Y., Guo, Y-Y., Zhang, Z., Zhou, J-J. and Zhang, Y-J. 2011. Functional characterization and immunolocalization of odorant binding protein 1 in the lucerne plant bug, Adelphocoris lineolatus (Goeze). Archives Of Insect Biochemistry And Physiology. 77 (2), pp. 81-98. https://doi.org/10.1002/arch.20427

AuthorsGu, S-H., Wang, W-X., Wang, G-R., Zhang, X-Y., Guo, Y-Y., Zhang, Z., Zhou, J-J. and Zhang, Y-J.
Abstract

In the insect phylum, the relationships between individuals and their environment are often modulated by chemical communication. Odorant binding proteins (OBPs) are widely and robustly expressed in insect olfactory organs and play a key role in chemosensing and transporting hydrophobic odorants across the sensillum lymph to the olfactory receptor neuron. In this study, a novel OBP gene (AlinOBP1) in the lucerne plant bug, Adelphocoris lineolatus was identified, cloned and expressed. Real-time PCR results indicated that the expression level of AlinOBP1 gene differed in each developmental stage (from first instar to adult) and was predominantly expressed in the antennae of adults. The expression level of AlinOBP1 was 1.91 times higher in male antennae than in female antennae. The binding properties of AlinOBP1 with 114 odorants were measured using a fluorescence probe, N-phenyl-1-naphthylamine (1-NPN), with fluorescence competitive binding. The results revealed that AlinOBP1 exhibits high binding abilities with two major putative pheromone components, ethyl butyrate and trans-2-hexenyl butyrate. In addition, it was observed that six volatiles released from cotton, octanal, nonanal, decanal, 2-ethyl-1-hexanol, beta-caryophyllene and beta-ionone also bind to AlinOBP1. Immunocytochemistry analysis showed that AlinOBP1 was expressed in the sensillum lymph of sensilla trichodica and sensilla basiconca. Our results demonstrate that AlinOBP1 may function as a carrier in the chemoperception of the lucerne plant bug. (C) 2011 Wiley Periodicals, Inc.

KeywordsBiochemistry & Molecular Biology; Entomology; Physiology
Year of Publication2011
JournalArchives Of Insect Biochemistry And Physiology
Journal citation77 (2), pp. 81-98
Digital Object Identifier (DOI)https://doi.org/10.1002/arch.20427
PubMed ID21541988
Open accessPublished as green open access
FunderNational Basic Research Program of China (973 Program)
National Natural Science Foundation of China
State High Technology Development Program
Funder project or codeCentre for Sustainable Pest and Disease Management (PDM)
Project: 5083
Publisher's version
ISSN07394462
0739-4462
PublisherWiley
Grant ID2007CB109202
31071694
30871640
2008AA02Z307

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