Pasteuria endospores from Heterodera cajani (Nematoda: Heteroderidae) exhibit inverted attachment and altered germination in cross-infection studies with Globodera pallida (Nematoda: Heteroderidae)

A - Papers appearing in refereed journals

Mohan, S., Mauchline, T. H., Rowe, J., Hirsch, P. R. and Davies, K. G. 2012. Pasteuria endospores from Heterodera cajani (Nematoda: Heteroderidae) exhibit inverted attachment and altered germination in cross-infection studies with Globodera pallida (Nematoda: Heteroderidae). FEMS Microbiology Ecology. 79 (3), pp. 675-684.

AuthorsMohan, S., Mauchline, T. H., Rowe, J., Hirsch, P. R. and Davies, K. G.
Abstract

The Pasteuria group of Gram-positive, endospore-forming bacteria are parasites of invertebrates and exhibit differences in host specificity. We describe a cross-infection study between an isolate of Pasteuria from pigeon pea cyst nematode, Heterodera cajani, which also infects the potato cyst nematode, Globodera pallida, from the United Kingdom. A proportion of the attached endospores, 13% on H. cajani and 22% on G. pallida adhere to the cuticle in an inverted orientation. Inverted and conventionally attached endospores germinated and produced bacillus-like rods that completed their life cycle in < 15 weeks within females of G. pallida. This is the first example in which the life cycle of a Pasteuria population was systematically followed in two different nematode genera. A 1430-base pair fragment of the 16S rRNA gene sequence of the Pasteuria isolate from H. cajani revealed 98.6% similarity to the orthologous gene in Pasteuria nishizawae. Additionally, their respective endospore sizes were not significantly different, in contrast their host ranges are. Potential reasons for this remain unclear and are discussed.

KeywordsMicrobiology
Year of Publication2012
JournalFEMS Microbiology Ecology
Journal citation79 (3), pp. 675-684
Digital Object Identifier (DOI)doi:10.1111/j.1574-6941.2011.01249.x
PubMed ID22092805
Open accessPublished as green open access
FunderBritish Council
Biotechnology and Biological Sciences Research Council
Funder project or codeSEF
Publisher's version
Output statusPublished
PublisherWiley
Oxford University Press (OUP)
Copyright licenseCC BY
ISSN0168-6496

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