Resistance to spiromesifen in Trialeurodes vaporariorum is associated with a single amino acid replacement in its target enzyme acetyl-coenzyme A carboxylase

A - Papers appearing in refereed journals

Karatolos, N., Williamson, M. S., Denholm, I., Gorman, K., Ffrench-Constant, R. H. and Nauen, R. 2012. Resistance to spiromesifen in Trialeurodes vaporariorum is associated with a single amino acid replacement in its target enzyme acetyl-coenzyme A carboxylase. Insect Molecular Biology. 21 (3), pp. 327-334. https://doi.org/10.1111/j.1365-2583.2012.01136.x

AuthorsKaratolos, N., Williamson, M. S., Denholm, I., Gorman, K., Ffrench-Constant, R. H. and Nauen, R.
Abstract

Spiromesifen is a novel insecticide and is classed as a tetronic acid derivative. It targets the insects' acetyl-coenzyme A carboxylase (ACCase) enzyme, causing a reduction in lipid biosynthesis. At the time of this publication, there are no reports of resistance to this class of insecticides in insects although resistance has been observed in several mite species. The greenhouse whitefly Trialeurodes vaporariorum (Westwood) is a serious pest of protected vegetable and ornamental crops in temperate regions of the world and spiromesifen is widely used in its control. Mortality rates of UK and European populations of T. vaporariorum to spiromesifen were calculated and up to 26-fold resistance was found. We therefore sought to examine the molecular mechanism underlying spiromesifen resistance in this important pest. Pre-treatment with piperonyl butoxide did not synergize spiromesifen, suggesting a target-site resistance mechanism. The full length ACCase gene was sequenced for a range of T. vaporariorum strains and a strong association was found between spiromesifen resistance and a glutamic acid substitution with lysine in position 645 (E645K) of this gene. A TaqMan allelic discrimination assay confirmed these findings. Although this resistance is not considered sufficient to compromise the field performance of spiromesifen, this association of E645K with resistance is the first report of a potential target site mechanism affecting an ACCase inhibitor in an arthropod species.

KeywordsBiochemistry & Molecular Biology; Entomology
Year of Publication2012
JournalInsect Molecular Biology
Journal citation21 (3), pp. 327-334
Digital Object Identifier (DOI)https://doi.org/10.1111/j.1365-2583.2012.01136.x
PubMed ID22458881
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeCentre for Sustainable Pest and Disease Management (PDM)
BBSRC CASE Quota Studentship: Molecular mechanisms of insecticide resistance in the glasshouse whitefly, Trialeurodes vaporariorum
Genetic and ecological determinants of insecticide resistance
PublisherWiley
ISSN0962-1075

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