The presence of two different target-site resistance mechanisms in individual plants of Alopecurus myosuroides Huds., identified using a quick molcular test for the characterisation of six ALS and seven ACCase SNPs

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Marshall, R., Hanley, S. J., Hull, R. I. and Moss, S. R. 2013. The presence of two different target-site resistance mechanisms in individual plants of Alopecurus myosuroides Huds., identified using a quick molcular test for the characterisation of six ALS and seven ACCase SNPs. Pest Management Science. 69 (6), pp. 727-737.

AuthorsMarshall, R., Hanley, S. J., Hull, R. I. and Moss, S. R.
Abstract

BACKGROUND Target-site resistance to ALS- and ACCase-inhibiting herbicides in the grass weed Alopecurus myosuroides is associated with well-characterised allelic variants encoding ALS- and ACCase-based resistance. The potential for combined ALS and ACCase resistance presents a threat to future control, given the extent to which these herbicides are used. The authors present a primer extension method for rapid detection of known resistance-conferring substitutions. RESULTS Individuals showing combined resistance to field-rate mesosulfuron + iodosulfuron and cycloxydim were identified in four field-collected populations, with proportions ranging from 30 to 100%. Genotyping with the SNaPshot primer extension kit showed the T197 and L574 ALS and L1781 ACCase isoforms to be associated with ALS and ACCase resistance whenever they occurred. CONCLUSION Combined ALS and ACCase target-site resistance threatens future control of A. myosuroides. The SNaPshot extension assay provides a reliable new multiplexable method for characterising known allelic variants of the ALS and ACCase genes of A. myosuroides. The method offers significant advantages over both CAPS/dCAPS and PASA in that full genotyping can be accomplished at any nucleotide position using a single extension primer. (c) 2012 Society of Chemical Industry

KeywordsAgronomy; Entomology
Year of Publication2013
JournalPest Management Science
Journal citation69 (6), pp. 727-737
Digital Object Identifier (DOI)doi:10.1002/ps.3429
PubMed ID23165793
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Department of Environment, Food and Rural Affairs
Funder project or codeDelivering Sustainable Systems (SS) [ISPG]
Combating herbicide resistance by developing and promoting more sustainable grass-weed control strategies
Output statusPublished
Publication dates
Online29 Oct 2012
Publication process dates
Accepted17 Oct 2012
Copyright licensePublisher copyright
PublisherWiley
ISSN1526-498X

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