The interactions between piperonyl butoxide and E4, a resistance-associated esterase from the peach-potato aphid, Myzus persicae Sulzer (Hemiptera: Aphididae)

A - Papers appearing in refereed journals

Philippou, D., Borzatta, V., Capparella, E., Moroni, L., Field, L. M. and Moores, G. D. 2013. The interactions between piperonyl butoxide and E4, a resistance-associated esterase from the peach-potato aphid, Myzus persicae Sulzer (Hemiptera: Aphididae). Pest Management Science. 69 (4), pp. 499-506.

AuthorsPhilippou, D., Borzatta, V., Capparella, E., Moroni, L., Field, L. M. and Moores, G. D.
Abstract

BACKGROUND: It has been reported previously that piperonyl butoxide (PBO) can inhibit both P450 and esterase activity. Although the method by which PBO combines with cytochrome P450 has been identified, the way in which it acts as an esterase inhibitor has not been established. This paper characterises the interactions between PBO and the resistance-associated esterase in Myzus persicae, E4. RESULTS: After incubation with PBO/analogues, hydrolysis of 1-naphthyl acetate by E4 is increased, but sequestration of azamethiphos is reduced. Rudimentary in silico modelling suggests PBO docks at the lip of the aromatic gorge. CONCLUSIONS: PBO binds with E4 to accelerate small substrates to the active-site triad, while acting as a blockade to larger, insecticidal molecules. Structureactivity studies with analogues of PBO also reveal the essential chemical moieties present in the molecule. (c) 2012 Society of Chemical Industry

KeywordsAgronomy; Entomology
Year of Publication2013
JournalPest Management Science
Journal citation69 (4), pp. 499-506
Digital Object Identifier (DOI)doi:10.1002/ps.3400
PubMed ID22969050
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDelivering Sustainable Systems (SS) [ISPG]
Output statusPublished
PublisherWiley
Copyright licenseCC BY
ISSN1526-498X

Permalink - https://repository.rothamsted.ac.uk/item/8qx24/the-interactions-between-piperonyl-butoxide-and-e4-a-resistance-associated-esterase-from-the-peach-potato-aphid-myzus-persicae-sulzer-hemiptera-aphididae

Restricted files

Publisher's version

Under embargo indefinitely

9 total views
1 total downloads
1 views this month
0 downloads this month