Transcriptome profiling of the whitefly Bemisia tabaci reveals stage-specific gene expression signatures for thiamethoxam resistance

A - Papers appearing in refereed journals

Yang, N., Xie, W., Jones, C. M., Bass, C., Jiao, X., Yang, X., Liu, B., Li, R. and Zhang, Y. 2013. Transcriptome profiling of the whitefly Bemisia tabaci reveals stage-specific gene expression signatures for thiamethoxam resistance. Insect Molecular Biology. 22 (5), pp. 485-496.

AuthorsYang, N., Xie, W., Jones, C. M., Bass, C., Jiao, X., Yang, X., Liu, B., Li, R. and Zhang, Y.
Abstract

Bemisia tabaci has developed high levels of resistance to many insecticides including the neonicotinoids and there is strong evidence that for some compounds resistance is stage-specific. To investigate the molecular basis of B.tabaci resistance to the neonicotinoid thiamethoxam we used a custom whitefly microarray to compare gene expression in the egg, nymph and adult stages of a thiamethoxam-resistant strain (TH-R) with a susceptible strain (TH-S). Gene ontology and bioinformatic analyses revealed that in all life stages many of the differentially expressed transcripts encoded enzymes involved in metabolic processes and/or metabolism of xenobiotics. Several of these are candidate resistance genes and include the cytochrome P450 CYP6CM1, which has been shown to confer resistance to several neonicotinoids previously, a P450 belonging to the Cytochrome P450s 4 family and a glutathione S-transferase (GST) belonging to the sigma class. Finally several ATP-binding cassette transporters of the ABCG subfamily were highly over-expressed in the adult stage of the TH-R strain and may play a role in resistance by active efflux. Here, we evaluated both common and stage-specific gene expression signatures and identified several candidate resistance genes that may underlie B.tabaci resistance to thiamethoxam.

KeywordsBiochemistry & Molecular Biology; Entomology
Year of Publication2013
JournalInsect Molecular Biology
Journal citation22 (5), pp. 485-496
Digital Object Identifier (DOI)doi:10.1111/imb.12038
PubMed ID23889345
Open accessPublished as ‘gold’ (paid) open access
FunderBeijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables, China
Beijing Science Foundation
China 863 Program
National Basic Research Program of China
Jiangsu Science for Distinguished Young Scholars
National Technology Support Program
Biotechnology and Biological Sciences Research Council
Funder project or codeDelivering Sustainable Systems (SS) [ISPG]
BBSRC Institute Career Path Fellowship: A genomic approach to understanding insecticide resistance in crop pests
Publisher's version
Output statusPublished
Publication dates
Online29 Jul 2013
Copyright licenseCC BY-NC
PublisherWiley
Grant ID31025020
2013CB127602
6131002
2012AA101502
2012BAD19B06
ISSN0962-1075

Permalink - https://repository.rothamsted.ac.uk/item/8qx86/transcriptome-profiling-of-the-whitefly-bemisia-tabaci-reveals-stage-specific-gene-expression-signatures-for-thiamethoxam-resistance

5 total views
2 total downloads
0 views this month
0 downloads this month