Environmentally driven evolution of Rubisco and improved photosynthesis and growth within the C3 genus Limonium (Plumbaginaceae)

A - Papers appearing in refereed journals

Galmes, J., Andralojc, P. J., Kapralov, M. V., Flexas, J., Keys, A. J., Molins, A., Parry, M. A. J. and Conesa, M. A. 2014. Environmentally driven evolution of Rubisco and improved photosynthesis and growth within the C3 genus Limonium (Plumbaginaceae). New Phytologist. 203 (3), pp. 989-999.

AuthorsGalmes, J., Andralojc, P. J., Kapralov, M. V., Flexas, J., Keys, A. J., Molins, A., Parry, M. A. J. and Conesa, M. A.
Abstract

Carbon assimilation by most ecosystems requires ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). Its kinetic parameters are likely to have evolved in parallel with intracellular CO2 availability, with the result that faster forms of Rubisco occur in species with CO2-concentrating mechanisms. The Rubisco catalytic properties were determined and evaluated in relation to growth and carbon assimilation capacity in Mediterranean Limonium species, inhabiting severe stress environments. Significant kinetic differences between closely related species depended on two amino acid substitutions at functionally important residues 309 and 328 within the Rubisco large subunit. The Rubisco of species facing the largest CO2 restrictions during drought had relatively high affinity for CO2 (low Michaelis-Menten constant for CO2 (K-c)) but low maximum rates of carboxylation (k(cat)(c)), while the opposite was found for species that maintained higher CO2 concentrations under similar conditions. Rubisco kinetic characteristics were correlated with photosynthetic rate in both well-watered and drought-stressed plants. Moreover, the drought-mediated decrease in plant biomass accumulation was consistently lower in species with higher Rubisco carboxylase catalytic efficiency (k(cat)(c)/K-c). The present study is the first demonstration of Rubisco adaptation during species diversification within closely related C-3 plants, revealing a direct relationship between Rubisco molecular evolution and the biomass accumulation of closely related species subjected to unfavourable conditions.

KeywordsPlant Sciences
Year of Publication2014
JournalNew Phytologist
Journal citation203 (3), pp. 989-999
Digital Object Identifier (DOI)doi:10.1111/nph.12858
PubMed ID24861241
Open accessPublished as bronze (free) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeWheat
[20:20 Wheat] Maximising yield potential of wheat
Publisher's version
PublisherWiley
Grant IDAGL2009-07999
BB/J/00426X/1
BB/I002545/1
BB/I017372/1
ISSN0028-646X

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