Virus induced gene silencing (VIGS) for functional anaylsis of wheat genes involved in Zymoseptoria tritici susceptibiblity and resistance

A - Papers appearing in refereed journals

Lee, W-S., Rudd, J. J. and Kanyuka, K. 2015. Virus induced gene silencing (VIGS) for functional anaylsis of wheat genes involved in Zymoseptoria tritici susceptibiblity and resistance. Fungal Genetics and Biology. 79 (June), pp. 84-88.

AuthorsLee, W-S., Rudd, J. J. and Kanyuka, K.
Abstract

Virus-induced gene silencing (VIGS) has emerged as a powerful reverse genetic technology in plants supplementary to stable transgenic RNAi and, in certain species, as a viable alternative approach for gene functional analysis. The RNA virus Barley stripe mosaic virus (BSMV) was developed as a VIGS vector in the early 2000s and since then it has been used to study the function of wheat genes.

Several variants of BSMV vectors are available, with some requiring in vitro transcription of infectious viral RNA, while others rely on in planta production of viral RNA from DNA-based vectors delivered to plant cells either by particle bombardment or Agrobacterium tumefaciens.

We adapted the latest generation of binary BSMV VIGS vectors for the identification and study of wheat genes of interest involved in interactions with Zymoseptoria tritici and here present detailed and the most up-to-date protocols.

KeywordsRNAi; Mycosphaerella graminicola; Septoria tritici; Wheat; Triticum aestivum; Virus induced gene silencing (VIGS); Functional genomics
Year of Publication2015
JournalFungal Genetics and Biology
Journal citation79 (June), pp. 84-88
Digital Object Identifier (DOI)doi:10.1016/j.fgb.2015.04.006
PubMed ID26092793
PubMed Central IDPMC4510315
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeWheat
[20:20 Wheat] Protecting yield potential of wheat
Publisher's version
Output statusPublished
Publication dates
Online16 Jun 2015
Publication process dates
Accepted08 Apr 2005
Copyright licenseCC BY
PublisherElsevier
Academic Press Inc Elsevier Science
ISSN1087-1845

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