A - Papers appearing in refereed journals
Lee, W-S., Rudd, J. J. and Kanyuka, K. 2015. Virus induced gene silencing (VIGS) for functional anaylsis of wheat genes involved in Zymoseptoria tritici susceptibiblity and resistance. Fungal Genetics and Biology. 79 (June), pp. 84-88. https://doi.org/10.1016/j.fgb.2015.04.006
|Authors||Lee, W-S., Rudd, J. J. and Kanyuka, K.|
Virus-induced gene silencing (VIGS) has emerged as a powerful reverse genetic technology in plants supplementary to stable transgenic RNAi and, in certain species, as a viable alternative approach for gene functional analysis. The RNA virus Barley stripe mosaic virus (BSMV) was developed as a VIGS vector in the early 2000s and since then it has been used to study the function of wheat genes.
Several variants of BSMV vectors are available, with some requiring in vitro transcription of infectious viral RNA, while others rely on in planta production of viral RNA from DNA-based vectors delivered to plant cells either by particle bombardment or Agrobacterium tumefaciens.
We adapted the latest generation of binary BSMV VIGS vectors for the identification and study of wheat genes of interest involved in interactions with Zymoseptoria tritici and here present detailed and the most up-to-date protocols.
|Keywords||RNAi; Mycosphaerella graminicola; Septoria tritici; Wheat; Triticum aestivum; Virus induced gene silencing (VIGS); Functional genomics|
|Year of Publication||2015|
|Journal||Fungal Genetics and Biology|
|Journal citation||79 (June), pp. 84-88|
|Digital Object Identifier (DOI)||https://doi.org/10.1016/j.fgb.2015.04.006|
|PubMed Central ID||PMC4510315|
|Open access||Published as ‘gold’ (paid) open access|
|Funder||Biotechnology and Biological Sciences Research Council|
|Funder project or code||Wheat|
|[20:20 Wheat] Protecting yield potential of wheat|
|Online||16 Jun 2015|
|Publication process dates|
|Accepted||08 Apr 2005|
|Copyright license||CC BY|
|Academic Press Inc Elsevier Science|
Permalink - https://repository.rothamsted.ac.uk/item/8v051/virus-induced-gene-silencing-vigs-for-functional-anaylsis-of-wheat-genes-involved-in-zymoseptoria-tritici-susceptibiblity-and-resistance