Unraveling incompatibility between wheat and the fungal pathogen Zymoseptoria tritici through apoplastic proteomics

A - Papers appearing in refereed journals

Yang, F., Li, W., Derbyshire, M., Larsen, M. R., Rudd, J. J. and Palmisano, G. 2015. Unraveling incompatibility between wheat and the fungal pathogen Zymoseptoria tritici through apoplastic proteomics. BMC Genomics. 16, p. 362.

AuthorsYang, F., Li, W., Derbyshire, M., Larsen, M. R., Rudd, J. J. and Palmisano, G.
Abstract

Background
Hemibiotrophic fungal pathogen Zymoseptoria tritici causes severe foliar disease in wheat. However, current knowledge of molecular mechanisms involved in plant resistance to Z. tritici and Z. tritici virulence factors is far from being complete. The present work investigated the proteome of leaf apoplastic fluid with emphasis on both host wheat and Z. tritici during the compatible and incompatible interactions.

Results
The proteomics analysis revealed rapid host responses to the biotrophic growth, including enhanced carbohydrate metabolism, apoplastic defenses and stress, and cell wall reinforcement, might contribute to resistance. Compatibility between the host and the pathogen was associated with inactivated plant apoplastic responses as well as fungal defenses to oxidative stress and perturbation of plant cell wall during the initial biotrophic stage, followed by the strong induction of plant defenses during the necrotrophic stage. To study the role of anti-oxidative stress in Z. tritici pathogenicity in depth, a YAP1 transcription factor regulating antioxidant expression was deleted and showed the contribution to anti-oxidative stress in Z. tritici, but was not required for pathogenicity. This result suggests the functional redundancy of antioxidants in the fungus.

Conclusions
The data demonstrate that incompatibility is probably resulted from the proteome-level activation of host apoplastic defenses as well as fungal incapability to adapt to stress and interfere with host cell at the biotrophic stage of the interaction.

Year of Publication2015
JournalBMC Genomics
Journal citation16, p. 362
Digital Object Identifier (DOI)doi:10.1186/s12864-015-1549-6
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeWheat
[20:20 Wheat] Protecting yield potential of wheat
BBSRC CASE Studentship: Investigating fungal pathogen metabolism through targeted analysis of the Mycosphaerella graminicola Cytochrome P
Publisher's version
Copyright license
CC BY
Output statusPublished
Publication dates
Online08 May 2015
Publication process dates
Accepted17 Apr 2015
ISSN1471-2164
PublisherBiomed Central Ltd

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