An alternative pathway for the effective production of the omega-3 long-chain polyunsaturates EPA and ETA in transgenic oilseeds

A - Papers appearing in refereed journals

Ruiz-Lopez, N., Haslam, R. P., Usher, S. L., Napier, J. A. and Sayanova, O. V. 2015. An alternative pathway for the effective production of the omega-3 long-chain polyunsaturates EPA and ETA in transgenic oilseeds. Plant Biotechnology Journal. 13 (9), pp. 1264-1275.

AuthorsRuiz-Lopez, N., Haslam, R. P., Usher, S. L., Napier, J. A. and Sayanova, O. V.
Abstract

The synthesis and accumulation of omega-3 long-chain polyunsaturated fatty acids in transgenic Camelina sativa is demonstrated using the so-called alternative pathway. This aerobic pathway is found in a small number of taxonomically unrelated unicellular organisms and utilizes a C18 Delta 9-elongase to generate C20 PUFAs. Here, we evaluated four different combinations of seed-specific transgene-derived activities to systematically determine the potential of this pathway to direct the synthesis of eicosapentaenoic acid (EPA) in transgenic plants. The accumulation of EPA and the related omega-3 LC-PUFA eicosatetraenoic acid (ETA) was observed up to 26.4% of total seed fatty acids, of which ETA was 9.5%. Seed oils such as these not only represent an additional source of EPA, but also an entirely new source of the bona fide fish oil ETA. Detailed lipidomic analysis of the alternative pathway in Camelina revealed that the acyl-substrate preferences of the different activities in the pathway can still generate a substrate-dichotomy bottleneck, largely due to inefficient acyl-exchange from phospholipids into the acyl-CoA pool. However, significant levels of EPA and ETA were detected in the triacylglycerols of transgenic seeds, confirming the channelling of these fatty acids into this storage lipid.

KeywordsBiotechnology & Applied Microbiology; Plant Sciences
Year of Publication2015
JournalPlant Biotechnology Journal
Journal citation13 (9), pp. 1264-1275
Digital Object Identifier (DOI)doi:10.1111/pbi.12328
PubMed ID25640865
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeCamelina
Publisher's version
PublisherWiley
ISSN1467-7644

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