Susceptibility of adult cat fleas (Siphonaptera: Pulicidae) to insecticides and status of insecticide resistance mutations at the Rdl and knockdown resistance loci

A - Papers appearing in refereed journals

Rust, M. K., Vetter, R., Denholm, I., Blagburn, B., Williamson, M. S., Kopp, S., Coleman, G., Hostetler, J., Davis, W., Mencke, N., Rees, R., Foit, S., Bohm, C. and Tetzner, K. 2015. Susceptibility of adult cat fleas (Siphonaptera: Pulicidae) to insecticides and status of insecticide resistance mutations at the Rdl and knockdown resistance loci. Parasitology Research. 114 (Supplement 1), pp. S7-S18.

AuthorsRust, M. K., Vetter, R., Denholm, I., Blagburn, B., Williamson, M. S., Kopp, S., Coleman, G., Hostetler, J., Davis, W., Mencke, N., Rees, R., Foit, S., Bohm, C. and Tetzner, K.
Abstract

The susceptibility of 12 field-collected isolates and 4 laboratory strains of cat fleas, Ctenocephalides felis was determined by topical application of some of the insecticides used as on-animal therapies to control them. In the tested field-collected flea isolates the LD50 values for fipronil and imidacloprid ranged from 0.09 to 0.35 ng/flea and 0.02 to 0.19 ng/flea, respectively, and were consistent with baseline figures published previously. The extent of variation in response to four pyrethroid insecticides differed between compounds with the LD50 values for deltamethrin ranging from 2.3 to 28.2 ng/flea, etofenprox ranging from 26.7 to 86.7 ng/flea, permethrin ranging from 17.5 to 85.6 ng/flea, and d-phenothrin ranging from 14.5 to 130 ng/flea. A comparison with earlier data for permethrin and deltamethrin implied a level of pyrethroid resistance in all isolates and strains. LD50 values for tetrachlorvinphos ranged from 20.0 to 420.0 ng/flea. The rdl mutation (conferring target-site resistance to cyclodiene insecticides) was present in most field-collected and laboratory strains, but had no discernible effect on responses to fipronil, which acts on the same receptor protein as cyclodienes. The kdr and skdr mutations conferring target-site resistance to pyrethroids but segregated in opposition to one another, precluding the formation of genotypes homozygous for both mutations.

KeywordsImidacloprid ; Deltamethrin; Etofenprox; Adult Flea; Cyclodiene Insecticide
Year of Publication2015
JournalParasitology Research
Journal citation114 (Supplement 1), pp. S7-S18
Digital Object Identifier (DOI)doi:10.1007/s00436-015-4512-1
PubMed ID26152407
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Bayer Animal Health GmbH
Funder project or codeThe Rothamsted Insect Survey [2012-2017]
Publisher's version
Output statusPublished
Publication dates
Online09 Jul 2015
PublisherSpringer
Copyright licenseCC BY
ISSN0932-0113

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