Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth Manduca sexta

A - Papers appearing in refereed journals

Kanost, M. R., Arrese, E. L., Cao, X., Chen, Y-R., Chellapilla, S. and Zhou, J-J. 2016. Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth Manduca sexta. Insect Biochemistry and Molecular Biology. 76 (September), pp. 118-147.

AuthorsKanost, M. R., Arrese, E. L., Cao, X., Chen, Y-R., Chellapilla, S. and Zhou, J-J.
Abstract

Manduca sexta, known as the tobacco hornworm or Carolina sphinx moth, is a lepidopteran insect that is used extensively as a model system for research in insect biochemistry, physiology, neurobiology, development, and immunity. One important benefit of this species as an experimental model is its extremely large size, reaching more than 10 g in the larval stage. M. sexta larvae feed on solanaceous plants and thus must tolerate a substantial challenge from plant allelochemicals, including nicotine. We report the sequence and annotation of the M. sexta genome, and a survey of gene expression in various tissues and developmental stages. The Msex_1.0 genome assembly resulted in a total genome size of 419.4 Mbp. Repetitive sequences accounted for 25.8% of the assembled genome. The official gene set is comprised of 15,451 protein-coding genes, of which 2498 were manually curated. Extensive RNA-seq data from many tissues and developmental stages were used to improve gene models and for insights into gene expression patterns. Genome wide synteny analysis indicated a high level of macrosynteny in the Lepidoptera. Annotation and analyses were carried out for gene families involved in a wide spectrum of biological processes, including apoptosis, vacuole sorting, growth and development, structures of exoskeleton, egg shells, and muscle, vision, chemosensation, ion channels, signal transduction, neuropeptide signaling, neurotransmitter synthesis and transport, nicotine tolerance, lipid metabolism, and immunity. This genome sequence, annotation, and analysis provide an important new resource from a well-studied model insect species and will facilitate further biochemical and mechanistic experimental studies of many biological systems in insects. 

Year of Publication2016
JournalInsect Biochemistry and Molecular Biology
Journal citation76 (September), pp. 118-147
Digital Object Identifier (DOI)doi:10.1016/j.ibmb.2016.07.005
Open accessPublished as non-open access
Funder project or codeDelivering Sustainable Systems (SS) [ISPG]
Innovative approaches to pest management
New pest resistance in rice, by breeding and genetic modification (GM) for constitutive and inducible levels of defence homoterpenes
UK-China Collaboration for production of biologically active homoisoprenoids and their eventual use for pest control
Output statusPublished
Publication dates
Online12 Aug 2016
Publication process dates
Accepted14 Jul 2016
PublisherElsevier
Elsevier
Copyright licensePublisher copyright
ISSN0965-1748

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