A - Papers appearing in refereed journals
Hamilton, M. L., Powers, S. J., Napier, J. A. and Sayanova, O. V. 2016. Heterotrophic Production of Omega-3 Long-Chain Polyunsaturated Fatty Acids by Trophically Converted Marine Diatom Phaeodactylum tricornutum. Marine Drugs. 14 (3), p. 53.
|Authors||Hamilton, M. L., Powers, S. J., Napier, J. A. and Sayanova, O. V.|
We have created via metabolic engineering a heterotrophic strain of Phaeodactylum tricornutum that accumulates enhanced levels of the high value omega-3 long chain polyunsaturated fatty acid (LC-PUFAs) docosahexaenoic acid (DHA). This was achieved by generation of transgenic strains in which the Δ5-elongase from Ostreococcus tauri was co-expressed with a glucose transporter from the moss Physcomitrella patens. This double transformant has the capacity to grow in the dark in liquid medium supplemented with glucose and accumulate substantial levels of omega-3 LC-PUFAs. The effects of glucose concentrations on growth and LC-PUFA production of wild type and transformed strains cultivated in the light and dark were studied. The highest omega-3 LC-PUFAs accumulation was observed in cultures grown under mixotrophic conditions in the presence of 1% glucose (up to 32.2% of total fatty acids, TFA). Both DHA and EPA are detected at high levels in the neutral lipids of transgenic cells grown under phototrophic conditions, averaging 36.5% and 23.6% of TFA, respectively. This study demonstrates the potential for P. tricornutum to be developed as a viable commercial strain for both EPA and DHA production under mixo- and heterotrophic conditions.
|Keywords||microalgae; Phaeodactylum tricornutum; omega-3 long chain polyunsaturated fatty acid; EPA; DHA; heterotrophic|
|Year of Publication||2016|
|Journal citation||14 (3), p. 53|
|Digital Object Identifier (DOI)||doi:10.3390/md14030053|
|Open access||Published as ‘gold’ (paid) open access|
|Funder||Biotechnology and Biological Sciences Research Council|
|Funder project or code||Designing Seeds for Nutrition and Health (DS)|
|Online||09 Mar 2016|
|Publication process dates|
|Accepted||26 Feb 2016|
|Copyright license||CC BY|
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