Heterotrophic Production of Omega-3 Long-Chain Polyunsaturated Fatty Acids by Trophically Converted Marine Diatom Phaeodactylum tricornutum

A - Papers appearing in refereed journals

Hamilton, M. L., Powers, S. J., Napier, J. A. and Sayanova, O. V. 2016. Heterotrophic Production of Omega-3 Long-Chain Polyunsaturated Fatty Acids by Trophically Converted Marine Diatom Phaeodactylum tricornutum. Marine Drugs. 14 (3), p. 53.

AuthorsHamilton, M. L., Powers, S. J., Napier, J. A. and Sayanova, O. V.
Abstract

We have created via metabolic engineering a heterotrophic strain of Phaeodactylum tricornutum that accumulates enhanced levels of the high value omega-3 long chain polyunsaturated fatty acid (LC-PUFAs) docosahexaenoic acid (DHA). This was achieved by generation of transgenic strains in which the Δ5-elongase from Ostreococcus tauri was co-expressed with a glucose transporter from the moss Physcomitrella patens. This double transformant has the capacity to grow in the dark in liquid medium supplemented with glucose and accumulate substantial levels of omega-3 LC-PUFAs. The effects of glucose concentrations on growth and LC-PUFA production of wild type and transformed strains cultivated in the light and dark were studied. The highest omega-3 LC-PUFAs accumulation was observed in cultures grown under mixotrophic conditions in the presence of 1% glucose (up to 32.2% of total fatty acids, TFA). Both DHA and EPA are detected at high levels in the neutral lipids of transgenic cells grown under phototrophic conditions, averaging 36.5% and 23.6% of TFA, respectively. This study demonstrates the potential for P. tricornutum to be developed as a viable commercial strain for both EPA and DHA production under mixo- and heterotrophic conditions.

Keywordsmicroalgae; Phaeodactylum tricornutum; omega-3 long chain polyunsaturated fatty acid; EPA; DHA; heterotrophic
Year of Publication2016
JournalMarine Drugs
Journal citation14 (3), p. 53
Digital Object Identifier (DOI)doi:10.3390/md14030053
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDesigning Seeds for Nutrition and Health (DS)
Publisher's version
Output statusPublished
Publication dates
Online09 Mar 2016
Publication process dates
Accepted26 Feb 2016
PublisherMDPI
MDPI
Copyright licenseCC BY
ISSN1660-3397

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