Heterotrophic Production of Omega-3 Long-Chain Polyunsaturated Fatty Acids by Trophically Converted Marine Diatom Phaeodactylum tricornutum

A - Papers appearing in refereed journals

Hamilton, M. L., Powers, S. J., Napier, J. A. and Sayanova, O. V. 2016. Heterotrophic Production of Omega-3 Long-Chain Polyunsaturated Fatty Acids by Trophically Converted Marine Diatom Phaeodactylum tricornutum. Marine Drugs. 14 (3), p. 53. https://doi.org/10.3390/md14030053

AuthorsHamilton, M. L., Powers, S. J., Napier, J. A. and Sayanova, O. V.
Abstract

We have created via metabolic engineering a heterotrophic strain of Phaeodactylum tricornutum that accumulates enhanced levels of the high value omega-3 long chain polyunsaturated fatty acid (LC-PUFAs) docosahexaenoic acid (DHA). This was achieved by generation of transgenic strains in which the Δ5-elongase from Ostreococcus tauri was co-expressed with a glucose transporter from the moss Physcomitrella patens. This double transformant has the capacity to grow in the dark in liquid medium supplemented with glucose and accumulate substantial levels of omega-3 LC-PUFAs. The effects of glucose concentrations on growth and LC-PUFA production of wild type and transformed strains cultivated in the light and dark were studied. The highest omega-3 LC-PUFAs accumulation was observed in cultures grown under mixotrophic conditions in the presence of 1% glucose (up to 32.2% of total fatty acids, TFA). Both DHA and EPA are detected at high levels in the neutral lipids of transgenic cells grown under phototrophic conditions, averaging 36.5% and 23.6% of TFA, respectively. This study demonstrates the potential for P. tricornutum to be developed as a viable commercial strain for both EPA and DHA production under mixo- and heterotrophic conditions.

Keywordsmicroalgae; Phaeodactylum tricornutum; omega-3 long chain polyunsaturated fatty acid; EPA; DHA; heterotrophic
Year of Publication2016
JournalMarine Drugs
Journal citation14 (3), p. 53
Digital Object Identifier (DOI)https://doi.org/10.3390/md14030053
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDesigning Seeds for Nutrition and Health (DS)
Project: 5319
Publisher's version
Output statusPublished
Publication dates
Online09 Mar 2016
Publication process dates
Accepted26 Feb 2016
PublisherMDPI
MDPI
Copyright licenseCC BY
ISSN1660-3397

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