A - Papers appearing in refereed journals
Hamilton, M. L., Powers, S. J., Napier, J. A. and Sayanova, O. V. 2016. Heterotrophic Production of Omega-3 Long-Chain Polyunsaturated Fatty Acids by Trophically Converted Marine Diatom Phaeodactylum tricornutum. Marine Drugs. 14 (3), p. 53. https://doi.org/10.3390/md14030053
Authors | Hamilton, M. L., Powers, S. J., Napier, J. A. and Sayanova, O. V. |
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Abstract | We have created via metabolic engineering a heterotrophic strain of Phaeodactylum tricornutum that accumulates enhanced levels of the high value omega-3 long chain polyunsaturated fatty acid (LC-PUFAs) docosahexaenoic acid (DHA). This was achieved by generation of transgenic strains in which the Δ5-elongase from Ostreococcus tauri was co-expressed with a glucose transporter from the moss Physcomitrella patens. This double transformant has the capacity to grow in the dark in liquid medium supplemented with glucose and accumulate substantial levels of omega-3 LC-PUFAs. The effects of glucose concentrations on growth and LC-PUFA production of wild type and transformed strains cultivated in the light and dark were studied. The highest omega-3 LC-PUFAs accumulation was observed in cultures grown under mixotrophic conditions in the presence of 1% glucose (up to 32.2% of total fatty acids, TFA). Both DHA and EPA are detected at high levels in the neutral lipids of transgenic cells grown under phototrophic conditions, averaging 36.5% and 23.6% of TFA, respectively. This study demonstrates the potential for P. tricornutum to be developed as a viable commercial strain for both EPA and DHA production under mixo- and heterotrophic conditions. |
Keywords | microalgae; Phaeodactylum tricornutum; omega-3 long chain polyunsaturated fatty acid; EPA; DHA; heterotrophic |
Year of Publication | 2016 |
Journal | Marine Drugs |
Journal citation | 14 (3), p. 53 |
Digital Object Identifier (DOI) | https://doi.org/10.3390/md14030053 |
Open access | Published as ‘gold’ (paid) open access |
Funder | Biotechnology and Biological Sciences Research Council |
Funder project or code | Designing Seeds for Nutrition and Health (DS) |
Project: 5319 | |
Publisher's version | |
Output status | Published |
Publication dates | |
Online | 09 Mar 2016 |
Publication process dates | |
Accepted | 26 Feb 2016 |
Publisher | MDPI |
MDPI | |
Copyright license | CC BY |
ISSN | 1660-3397 |
Permalink - https://repository.rothamsted.ac.uk/item/8v363/heterotrophic-production-of-omega-3-long-chain-polyunsaturated-fatty-acids-by-trophically-converted-marine-diatom-phaeodactylum-tricornutum