Induced thiacloprid insensitivity in honeybees (Apis mellifera L.) is associated with up-regulation of detoxification genes

A - Papers appearing in refereed journals

Alptekin, S., Bass, C., Nicholls, C., Paine, M. J. I., Clark, S. J., Field, L. and Moores, G. D. 2016. Induced thiacloprid insensitivity in honeybees (Apis mellifera L.) is associated with up-regulation of detoxification genes. Insect Molecular Biology. 25 (2), pp. 171-180.

AuthorsAlptekin, S., Bass, C., Nicholls, C., Paine, M. J. I., Clark, S. J., Field, L. and Moores, G. D.
Abstract

Honey bees, Apis mellifera, are markedly less sensitive to neonicotinoid insecticides containing a cyanoimino pharmacophore than to those with a nitroimino group. Although previous work has suggested that this results from enhanced metabolism of the former by detoxification enzymes, the specific enzyme(s) involved remain to be characterized. In this work, a pretreatment of honey bees with a sublethal dose of thiacloprid resulted in induced insensitivity to the same compound immediately following thiacloprid feeding. A longer pretreatment time resulted in no, or increased, sensitivity. Transcriptome profiling, using microarrays, identified a number of genes encoding detoxification enzymes that were over-expressed significantly in insecticide-treated bees compared with untreated controls. These included five P450s, CYP6BE1, CYP305D1, CYP6AS5, CYP315A1, CYP301A1, and a carboxyl/cholinesterase (CCE) CCE8. Four of these P450s were functionally expressed in Escherichia coli and their ability to metabolize thiacloprid examined by liquid chromatography-mass spectrometry (LC-MS) analysis.

KeywordsBiochemistry & Molecular Biology; Entomology
Year of Publication2016
JournalInsect Molecular Biology
Journal citation25 (2), pp. 171-180
Digital Object Identifier (DOI)doi:10.1111/imb.12211
PubMed ID26790026
Open accessPublished as non-open access
FunderAHDB Cereals and Oilseeds, a division of the Agriculture and Horticulture Development Board, UK
Biotechnology and Biological Sciences Research Council
Agriculture and Horticulture Development Board
Funder project or codeBBSRC Quota Studentship: Investigating the molecular mechanisms of insecticide resistance in the tomato leafminer Tuta absoluta
Output statusPublished
Publication dates
Online10 Jan 2016
PublisherWiley
ISSN0962-1075

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